======= APAF1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: APAF1
  * **<color #00a2e8>Official Name</color>**: apoptotic peptidase activating factor 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=317|317]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O14727|O14727]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=APAF1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20APAF1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602233|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WD40|
|NB-ARC|
|CARD|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|response to G1 DNA damage checkpoint signaling|
|response to mitotic cell cycle checkpoint signaling|
|apoptosome|
|striated muscle cell apoptotic process|
|cardiac muscle cell apoptotic process|
|muscle cell apoptotic process|
|response to DNA damage checkpoint signaling|
|response to DNA integrity checkpoint signaling|
|response to cell cycle checkpoint signaling|
|activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c|
|regulation of apoptotic DNA fragmentation|
|regulation of DNA catabolic process|
|cysteine-type endopeptidase activator activity involved in apoptotic process|
|intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress|
|ADP binding|
|negative regulation of G0 to G1 transition|
|regulation of execution phase of apoptosis|
|regulation of G0 to G1 transition|
|neuron apoptotic process|
|neuron death|
|heat shock protein binding|
|nucleotide binding|
|activation of cysteine-type endopeptidase activity involved in apoptotic process|
|neural tube closure|
|tube closure|
|primary neural tube formation|
|neural tube formation|
|secretory granule lumen|
|embryonic epithelial tube formation|
|ficolin-1-rich granule lumen|
|epithelial tube formation|
|positive regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|morphogenesis of embryonic epithelium|
|tube formation|
|intrinsic apoptotic signaling pathway|
|positive regulation of cysteine-type endopeptidase activity|
|cellular response to transforming growth factor beta stimulus|
|neural tube development|
|response to transforming growth factor beta|
|positive regulation of endopeptidase activity|
|positive regulation of apoptotic signaling pathway|
|positive regulation of peptidase activity|
|cellular response to biotic stimulus|
|response to nutrient|
|regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|regulation of cysteine-type endopeptidase activity|
|response to endoplasmic reticulum stress|
|kidney development|
|aging|
|renal system development|
|apoptotic signaling pathway|
|epithelial tube morphogenesis|
|urogenital system development|
|negative regulation of cell cycle process|
|protein homooligomerization|
|response to hypoxia|
|regulation of DNA metabolic process|
|positive regulation of proteolysis|
|response to decreased oxygen levels|
|response to oxygen levels|
|forebrain development|
|regulation of apoptotic signaling pathway|
|regulation of endopeptidase activity|
|morphogenesis of an epithelium|
|regulation of peptidase activity|
|neutrophil degranulation|
|neutrophil activation involved in immune response|
|neutrophil mediated immunity|
|response to nutrient levels|
|cellular response to growth factor stimulus|
|neutrophil activation|
|granulocyte activation|
|leukocyte degranulation|
|myeloid leukocyte mediated immunity|
|protein complex oligomerization|
|myeloid cell activation involved in immune response|
|response to extracellular stimulus|
|response to growth factor|
|tissue morphogenesis|
|embryonic morphogenesis|
|negative regulation of cell cycle|
|myeloid leukocyte activation|
|protein-containing complex|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|chordate embryonic development|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|embryo development ending in birth or egg hatching|
|tube morphogenesis|
|positive regulation of cell death|
|regulated exocytosis|
|regulation of proteolysis|
|brain development|
|regulation of cell cycle process|
|leukocyte mediated immunity|
|positive regulation of hydrolase activity|
|head development|
|exocytosis|
|regulation of cellular catabolic process|
|tube development|
|anatomical structure formation involved in morphogenesis|
|apoptotic process|
|leukocyte activation|
|embryo development|
|central nervous system development|
|regulation of catabolic process|
|secretion by cell|
|export from cell|
|programmed cell death|
|identical protein binding|
|cell activation|
|cell death|
|immune effector process|
|epithelium development|
|secretion|
|response to abiotic stimulus|
|regulation of cell cycle|
|cellular response to endogenous stimulus|
|regulation of hydrolase activity|
|response to biotic stimulus|
|positive regulation of catalytic activity|
|response to endogenous stimulus|
|ATP binding|
|regulation of apoptotic process|
|protein-containing complex assembly|
|regulation of programmed cell death|
|positive regulation of cellular protein metabolic process|
|positive regulation of signal transduction|
|regulation of cell death|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|tissue development|
|positive regulation of molecular function|
|positive regulation of cell communication|
|positive regulation of signaling|
|protein-containing complex subunit organization|
|immune response|
|extracellular region|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|1.7|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|1.72|
|[[:results:exp437|Ethanol 0.003 R08 exp437]]|1.72|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|1.73|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|1.73|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|1.75|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|1.76|
|[[:results:exp507|Monensin 0.3μM R08 exp507]]|1.78|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|1.84|
|[[:results:exp355|Dinaciclib 0.007μM R07 exp355]]|1.84|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|1.84|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|1.87|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|1.88|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|1.89|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|1.94|
|[[:results:exp330|5-Azacytidine 2μM R07 exp330]]|1.98|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|1.99|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|1.99|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|2.02|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|2.11|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|2.13|
|[[:results:exp292|Menadione 5μM R06 exp292]]|2.39|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|2.42|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|2.45|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|2.47|
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|2.55|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|2.56|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|2.57|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|2.64|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|2.66|
|[[:results:exp17|DABN 20μM R00 exp17]]|2.73|
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|2.92|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|2.96|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|2.98|
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|3.04|
|[[:results:exp116|AICAR 240μM R03 exp116]]|3.15|
|[[:results:exp20|Etoposide 10μM R00 exp20]]|3.16|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|3.23|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|3.25|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|3.33|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|3.47|
|[[:results:exp211|AICAR 240μM R05 exp211]]|3.61|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|3.65|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|3.7|
|[[:results:exp134|MS023 2μM R03 exp134]]|3.74|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|3.79|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|3.81|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|3.84|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|4.18|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|4.19|
|[[:results:exp474|CR131-b 0.005μM R08 exp474]]|4.47|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|4.55|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|4.67|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|4.97|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|5.05|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|5.2|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|5.39|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|5.65|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|5.81|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|5.85|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|5.96|
|[[:results:exp135|MS023 7μM R03 exp135]]|5.97|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|6.23|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|6.28|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:b:bak1|BAK1]]|0.82|
|[[:human genes:c:casp9|CASP9]]|0.79|
|[[:human genes:v:vdac2|VDAC2]]|0.756|
|[[:human genes:p:plaa|PLAA]]|0.756|
|[[:human genes:m:march5|MARCH5]]|0.744|
|[[:human genes:c:cycs|CYCS]]|0.742|
|[[:human genes:u:ubqln1|UBQLN1]]|0.726|
|[[:human genes:h:hccs|HCCS]]|0.722|
|[[:human genes:d:diablo|DIABLO]]|0.691|
|[[:human genes:f:faf2|FAF2]]|0.653|
|[[:human genes:h:hars|HARS]]|0.635|
|[[:human genes:d:dohh|DOHH]]|0.629|
|[[:human genes:h:hspd1|HSPD1]]|0.619|
|[[:human genes:s:serf2|SERF2]]|0.602|
|[[:human genes:e:eny2|ENY2]]|0.584|
|[[:human genes:m:mfn1|MFN1]]|0.576|
|[[:human genes:a:atad1|ATAD1]]|0.575|
|[[:human genes:b:bap1|BAP1]]|0.573|
|[[:human genes:t:triap1|TRIAP1]]|0.572|
|[[:human genes:r:romo1|ROMO1]]|0.561|
|[[:human genes:c:crls1|CRLS1]]|0.56|
|[[:human genes:t:thg1l|THG1L]]|0.556|
|[[:human genes:u:uap1|UAP1]]|0.554|
|[[:human genes:r:rptor|RPTOR]]|0.547|
|[[:human genes:s:stau1|STAU1]]|0.539|
|[[:human genes:g:gabpa|GABPA]]|0.536|
|[[:human genes:g:gfpt1|GFPT1]]|0.535|
|[[:human genes:c:csnk2b|CSNK2B]]|0.534|
|[[:human genes:p:ppp2r3c|PPP2R3C]]|0.533|
|[[:human genes:m:metap2|METAP2]]|0.517|
|[[:human genes:m:mtor|MTOR]]|0.517|
|[[:human genes:g:gmppb|GMPPB]]|0.516|
|[[:human genes:s:srp14|SRP14]]|0.51|
|[[:human genes:p:ppa2|PPA2]]|0.507|
|[[:human genes:r:rad23b|RAD23B]]|0.505|
|[[:human genes:m:meaf6|MEAF6]]|0.504|
|[[:human genes:u:ube2k|UBE2K]]|0.504|
|[[:human genes:p:pmm2|PMM2]]|0.501|
|[[:human genes:t:taz|TAZ]]|0.498|
|[[:human genes:k:kdsr|KDSR]]|0.497|
|[[:human genes:d:ddhd2|DDHD2]]|0.493|
|[[:human genes:v:vhl|VHL]]|0.487|
|[[:human genes:a:atp5j|ATP5J]]|0.486|
|[[:human genes:s:sec61g|SEC61G]]|0.481|
|[[:human genes:t:tamm41|TAMM41]]|0.479|
|[[:human genes:p:pisd|PISD]]|0.476|
|[[:human genes:r:rnmt|RNMT]]|0.474|
|[[:human genes:o:opa1|OPA1]]|0.473|
|[[:human genes:r:rtcb|RTCB]]|0.473|
|[[:human genes:m:mlst8|MLST8]]|0.471|
|[[:human genes:u:ube2g2|UBE2G2]]|0.471|
|[[:human genes:d:dpagt1|DPAGT1]]|0.47|
|[[:human genes:n:nsd1|NSD1]]|0.466|
|[[:human genes:c:ctdnep1|CTDNEP1]]|0.465|
|[[:human genes:a:atp2a2|ATP2A2]]|0.463|
|[[:human genes:u:uba3|UBA3]]|0.462|
|[[:human genes:a:atxn10|ATXN10]]|0.458|
|[[:human genes:n:nae1|NAE1]]|0.456|
|[[:human genes:a:alg1|ALG1]]|0.454|
|[[:human genes:p:pik3r4|PIK3R4]]|0.454|
|[[:human genes:g:gtf3a|GTF3A]]|0.45|
|[[:human genes:a:alg2|ALG2]]|0.448|
|[[:human genes:c:cbfb|CBFB]]|0.448|
|[[:human genes:f:fech|FECH]]|0.447|
|[[:human genes:a:abcf1|ABCF1]]|0.447|
|[[:human genes:r:rpn1|RPN1]]|0.443|
|[[:human genes:p:pam16|PAM16]]|0.442|
|[[:human genes:t:timm13|TIMM13]]|0.441|
|[[:human genes:n:nxt1|NXT1]]|0.44|
|[[:human genes:w:wdr59|WDR59]]|0.439|
|[[:human genes:e:eif3f|EIF3F]]|0.439|
|[[:human genes:w:wdr24|WDR24]]|0.438|
|[[:human genes:a:atp5i|ATP5I]]|0.437|
|[[:human genes:z:zzz3|ZZZ3]]|0.436|
|[[:human genes:p:pdpk1|PDPK1]]|0.433|
|[[:human genes:a:atp5o|ATP5O]]|0.431|
|[[:human genes:a:atp5a1|ATP5A1]]|0.43|
|[[:human genes:n:nupl2|NUPL2]]|0.43|
|[[:human genes:c:cept1|CEPT1]]|0.429|
|[[:human genes:g:gtf3c6|GTF3C6]]|0.427|
|[[:human genes:b:brwd1|BRWD1]]|0.427|
|[[:human genes:t:tp53|TP53]]|0.427|
|[[:human genes:d:dhps|DHPS]]|0.424|
|[[:human genes:e:exoc4|EXOC4]]|0.423|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.423|
|[[:human genes:s:sec16a|SEC16A]]|0.419|
|[[:human genes:a:alg5|ALG5]]|0.418|
|[[:human genes:t:trim28|TRIM28]]|0.416|
|[[:human genes:p:ppp5c|PPP5C]]|0.415|
|[[:human genes:p:pelo|PELO]]|0.414|
|[[:human genes:a:atp5f1|ATP5F1]]|0.413|
|[[:human genes:c:cars|CARS]]|0.412|
|[[:human genes:s:slc25a26|SLC25A26]]|0.411|
|[[:human genes:s:slmo2|SLMO2]]|0.411|
|[[:human genes:d:dad1|DAD1]]|0.411|
|[[:human genes:t:tbca|TBCA]]|0.41|
|[[:human genes:e:eif4e|EIF4E]]|0.409|
|[[:human genes:r:rbmx2|RBMX2]]|0.408|
|[[:human genes:s:srrd|SRRD]]|0.407|
|[[:human genes:a:atp5b|ATP5B]]|0.407|
|[[:human genes:u:ube2m|UBE2M]]|0.406|
|[[:human genes:g:gnas|GNAS]]|0.403|
|[[:human genes:k:kctd5|KCTD5]]|0.402|
|[[:human genes:f:fbxo11|FBXO11]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 16386
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.79 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='APAF1 Expression in NALM6 Cells: 5.79'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>