======= DEPDC5 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DEPDC5
  * **<color #00a2e8>Official Name</color>**: DEP domain containing 5, GATOR1 subcomplex subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9681|9681]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O75140|O75140]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DEPDC5&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DEPDC5|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/614191|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]. 
  * **<color #00a2e8>UniProt Summary</color>**:  As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway. {ECO:0000269|PubMed:23723238, ECO:0000269|PubMed:25457612}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DEP|
|DUF3608|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|GATOR1 complex|
|negative regulation of TORC1 signaling|
|Cul3-RING ubiquitin ligase complex|
|regulation of TORC1 signaling|
|cellular response to amino acid starvation|
|response to amino acid starvation|
|negative regulation of TOR signaling|
|regulation of TOR signaling|
|cellular response to starvation|
|response to starvation|
|cellular response to nutrient levels|
|lysosome|
|cellular response to extracellular stimulus|
|protein-containing complex binding|
|GTPase activator activity|
|lysosomal membrane|
|cellular response to external stimulus|
|positive regulation of GTPase activity|
|regulation of GTPase activity|
|response to nutrient levels|
|negative regulation of intracellular signal transduction|
|response to extracellular stimulus|
|perinuclear region of cytoplasm|
|positive regulation of hydrolase activity|
|negative regulation of signal transduction|
|regulation of hydrolase activity|
|negative regulation of cell communication|
|negative regulation of signaling|
|positive regulation of catalytic activity|
|negative regulation of response to stimulus|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of molecular function|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|-3.9|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-3.59|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-3.4|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-3.11|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|-2.91|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|-2.89|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.85|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-2.71|
|[[:results:exp223|Cabazitaxel 0.001μM R05 exp223]]|-2.69|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|-2.62|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-2.6|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-2.58|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-2.44|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-2.42|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-2.38|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-2.27|
|[[:results:exp464|Calcipotriol 5μM R08 exp464]]|-2.26|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.25|
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|-2.25|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-2.23|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|-2.21|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-2.2|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-2.19|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|-2.16|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|-2.16|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|-2.1|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|-2.05|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|-2.03|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|-2.03|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|-2.02|
|[[:results:exp59|UMK57 1μM R01 exp59]]|-2.02|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|-1.99|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|-1.98|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|-1.98|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|-1.98|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-1.96|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-1.96|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|-1.95|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|-1.94|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|-1.92|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|-1.91|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|-1.91|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-1.89|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|-1.87|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-1.86|
|[[:results:exp249|Vinorelbine 0.001μM R05 exp249]]|-1.86|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|-1.84|
|[[:results:exp275|Citral 75μM R06 exp275]]|-1.83|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-1.83|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-1.82|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-1.76|
|[[:results:exp286|HMS-I2 1μM R06 exp286]]|-1.74|
|[[:results:exp289|Hydroxyurea 15μM R06 exp289]]|-1.74|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|-1.74|
|[[:results:exp73|LJH685 5μM R02 exp73]]|-1.73|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|-1.73|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|-1.72|
|[[:results:exp287|HMS-I2 5μM R06 exp287]]|-1.72|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-1.7|
|[[:results:exp534|Trientine 500μM R08 exp534]]|1.71|
|[[:results:exp124|GSK343 3μM R03 exp124]]|1.75|
|[[:results:exp465|Cannabidiol 13μM R08 exp465]]|1.79|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|1.8|
|[[:results:exp218|A-395 10μM R05 exp218]]|1.94|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|2.01|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|2.05|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.09|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|2.13|
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|2.13|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|2.16|
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|2.26|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|2.57|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|2.59|
|[[:results:exp389|PF-06409577 20μM R07 exp389]]|2.88|
|[[:results:exp452|Azithromycin 100μM R08 exp452]]|3.12|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|3.56|
|[[:results:exp151|SGC0946 7μM R03 exp151]]|3.57|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|4.56|
|[[:results:exp504|MK2206 4μM R08 exp504]]|6.01|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:n:nprl3|NPRL3]]|0.839|
|[[:human genes:n:nprl2|NPRL2]]|0.808|
|[[:human genes:s:szt2|SZT2]]|0.784|
|[[:human genes:k:kptn|KPTN]]|0.714|
|[[:human genes:i:itfg2|ITFG2]]|0.664|
|[[:human genes:c:c12orf66|C12orf66]]|0.65|
|[[:human genes:t:tsc2|TSC2]]|0.628|
|[[:human genes:s:sesn2|SESN2]]|0.598|
|[[:human genes:t:tsc1|TSC1]]|0.573|
|[[:human genes:a:anp32a|ANP32A]]|0.454|
|[[:human genes:c:ccdc130|CCDC130]]|0.454|
|[[:human genes:z:zfp36l2|ZFP36L2]]|0.44|
|[[:human genes:c:cnot2|CNOT2]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9249
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.36 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DEPDC5 Expression in NALM6 Cells: 5.36'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>