======= GCLM =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GCLM
  * **<color #00a2e8>Official Name</color>**: glutamate-cysteine ligase modifier subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2730|2730]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P48507|P48507]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GCLM&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GCLM|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601176|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Aldo ket red|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of glutamate-cysteine ligase activity|
|regulation of glutamate-cysteine ligase activity|
|glutamate-cysteine ligase catalytic subunit binding|
|cellular response to L-phenylalanine derivative|
|glutamate-cysteine ligase activity|
|hepatic stellate cell activation|
|glutamate-cysteine ligase complex|
|cellular response to thyroxine stimulus|
|response to human chorionic gonadotropin|
|response to L-phenylalanine derivative|
|response to thyroxine|
|fibroblast activation|
|positive regulation of ligase activity|
|response to nitrosative stress|
|regulation of ligase activity|
|cysteine metabolic process|
|cellular response to follicle-stimulating hormone stimulus|
|glutathione biosynthetic process|
|cellular response to thyroid hormone stimulus|
|cellular response to hepatocyte growth factor stimulus|
|enzyme regulator activity|
|response to follicle-stimulating hormone|
|nonribosomal peptide biosynthetic process|
|response to hepatocyte growth factor|
|cellular response to gonadotropin stimulus|
|regulation of mitochondrial depolarization|
|response to thyroid hormone|
|response to gonadotropin|
|glutamate metabolic process|
|sulfur amino acid metabolic process|
|serine family amino acid metabolic process|
|regulation of membrane depolarization|
|cellular modified amino acid biosynthetic process|
|glutathione metabolic process|
|apoptotic mitochondrial changes|
|response to activity|
|cellular response to glucose stimulus|
|cellular response to amino acid stimulus|
|regulation of mitochondrial membrane potential|
|glutamine family amino acid metabolic process|
|cellular response to hexose stimulus|
|cellular response to monosaccharide stimulus|
|cellular response to carbohydrate stimulus|
|cellular glucose homeostasis|
|dicarboxylic acid metabolic process|
|cellular response to leukemia inhibitory factor|
|response to leukemia inhibitory factor|
|negative regulation of extrinsic apoptotic signaling pathway|
|cellular response to fibroblast growth factor stimulus|
|response to amino acid|
|response to fibroblast growth factor|
|response to glucose|
|regulation of blood vessel diameter|
|regulation of tube diameter|
|regulation of tube size|
|response to hexose|
|negative regulation of neuron apoptotic process|
|response to monosaccharide|
|regulation of extrinsic apoptotic signaling pathway|
|vascular process in circulatory system|
|response to carbohydrate|
|sulfur compound biosynthetic process|
|glucose homeostasis|
|carbohydrate homeostasis|
|cellular modified amino acid metabolic process|
|negative regulation of neuron death|
|regulation of neuron apoptotic process|
|cellular response to acid chemical|
|alpha-amino acid metabolic process|
|cofactor biosynthetic process|
|response to nutrient|
|negative regulation of apoptotic signaling pathway|
|aging|
|cellular amino acid metabolic process|
|regulation of neuron death|
|response to acid chemical|
|sulfur compound metabolic process|
|response to oxidative stress|
|blood circulation|
|circulatory system process|
|regulation of apoptotic signaling pathway|
|cellular response to drug|
|peptide biosynthetic process|
|regulation of membrane potential|
|cofactor metabolic process|
|mitochondrion organization|
|protein heterodimerization activity|
|response to nutrient levels|
|cellular response to growth factor stimulus|
|regulation of anatomical structure size|
|amide biosynthetic process|
|peptide metabolic process|
|response to extracellular stimulus|
|response to growth factor|
|cellular response to organic cyclic compound|
|cellular response to organonitrogen compound|
|cellular response to hormone stimulus|
|cellular response to nitrogen compound|
|cellular chemical homeostasis|
|ion homeostasis|
|cellular amide metabolic process|
|negative regulation of apoptotic process|
|cellular homeostasis|
|negative regulation of programmed cell death|
|response to hormone|
|carboxylic acid metabolic process|
|response to organic cyclic compound|
|apoptotic process|
|negative regulation of cell death|
|response to organonitrogen compound|
|oxoacid metabolic process|
|cellular response to cytokine stimulus|
|response to drug|
|organic acid metabolic process|
|programmed cell death|
|cellular response to oxygen-containing compound|
|cell activation|
|response to nitrogen compound|
|cell death|
|response to cytokine|
|chemical homeostasis|
|cellular response to endogenous stimulus|
|negative regulation of signal transduction|
|negative regulation of cell communication|
|negative regulation of signaling|
|organonitrogen compound biosynthetic process|
|positive regulation of catalytic activity|
|response to endogenous stimulus|
|regulation of apoptotic process|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|negative regulation of response to stimulus|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|regulation of cell death|
|small molecule metabolic process|
|positive regulation of molecular function|
|system process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp340|BN82002 4μM R07 exp340]]|-4.27|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-4.1|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-4.01|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-3.95|
|[[:results:exp449|Arsenic trioxide 60μM R08 exp449]]|-3.74|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|-3.55|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|-3.53|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-3.38|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-3.37|
|[[:results:exp145|PNU96415E 10μM R03 exp145]]|-3.33|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-3.32|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|-3.29|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|-3.25|
|[[:results:exp292|Menadione 5μM R06 exp292]]|-3.21|
|[[:results:exp239|PFI-2 4μM R05 exp239]]|-3.21|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-3.15|
|[[:results:exp169|BH1 1μM R04 exp169]]|-2.98|
|[[:results:exp133|MKC9989 10μM R03 exp133]]|-2.76|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-2.67|
|[[:results:exp187|proTAME 5μM R04 exp187]]|-2.67|
|[[:results:exp455|Benzoate 10000μM R08 exp455]]|-2.53|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|-2.46|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.35|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|-2.34|
|[[:results:exp237|NN-Diethyl-meta-toluamide 100μM R05 exp237]]|-2.23|
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|-2.22|
|[[:results:exp281|Disulfiram 4.3μM R06 exp281]]|-2.22|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|-2.17|
|[[:results:exp539|42°C R08 exp539]]|-2.09|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|-2.09|
|[[:results:exp219|A-395N 10μM R05 exp219]]|-2.07|
|[[:results:exp144|PFI-3 10μM R03 exp144]]|-2.03|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-2.02|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|-2.02|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|-1.96|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|-1.91|
|[[:results:exp362|GSK-J4 1μM R07 exp362]]|-1.82|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-1.8|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-1.79|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|-1.77|
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|-1.76|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|-1.76|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|-1.73|
|[[:results:exp20|Etoposide 10μM R00 exp20]]|-1.71|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-1.71|
|[[:results:exp432|YM155 0.001μM R08 exp432]]|-1.71|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|1.71|
|[[:results:exp348|Cyclosporin-A 3μM R07 exp348]]|1.79|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|1.82|
|[[:results:exp347|Cyclosporin-A 0.8μM R07 exp347]]|1.96|
|[[:results:exp433|LJH685 50μM R08 exp433]]|2.23|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|3.2|
|[[:results:exp518|RK-33 8μM R08 exp518]]|8.49|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:g:gss|GSS]]|0.751|
|[[:human genes:g:gclc|GCLC]]|0.679|
|[[:human genes:o:osgin2|OSGIN2]]|0.64|
|[[:human genes:s:slc7a11|SLC7A11]]|0.428|
|[[:human genes:l:lrp8|LRP8]]|0.415|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 4927
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.89 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GCLM Expression in NALM6 Cells: 4.89'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>