======= LEO1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: LEO1
  * **<color #00a2e8>Official Name</color>**: LEO1 homolog, Paf1/RNA polymerase II complex component
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=123169|123169]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8WVC0|Q8WVC0]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=LEO1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20LEO1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610507|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling. {ECO:0000269|PubMed:15632063, ECO:0000269|PubMed:15791002, ECO:0000269|PubMed:19345177, ECO:0000269|PubMed:19952111, ECO:0000269|PubMed:20178742}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Leo1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|RNA polymerase II C-terminal domain phosphoserine binding|
|Cdc73/Paf1 complex|
|histone H2B ubiquitination|
|positive regulation of mRNA 3-end processing|
|endodermal cell fate commitment|
|positive regulation of transcription elongation from RNA polymerase II promoter|
|mRNA polyadenylation|
|cell fate commitment involved in formation of primary germ layer|
|regulation of mRNA 3-end processing|
|beta-catenin-TCF complex assembly|
|RNA polyadenylation|
|histone monoubiquitination|
|regulation of transcription elongation from RNA polymerase II promoter|
|positive regulation of mRNA processing|
|positive regulation of DNA-templated transcription, elongation|
|histone ubiquitination|
|endodermal cell differentiation|
|endoderm formation|
|regulation of DNA-templated transcription, elongation|
|protein monoubiquitination|
|endoderm development|
|transcription elongation from RNA polymerase II promoter|
|negative regulation of myeloid cell differentiation|
|positive regulation of mRNA metabolic process|
|mRNA 3-end processing|
|DNA-templated transcription, elongation|
|formation of primary germ layer|
|fibrillar center|
|RNA 3-end processing|
|stem cell population maintenance|
|negative regulation of hemopoiesis|
|maintenance of cell number|
|regulation of mRNA processing|
|gastrulation|
|regulation of myeloid cell differentiation|
|cell fate commitment|
|regulation of mRNA metabolic process|
|Wnt signaling pathway|
|cell-cell signaling by wnt|
|histone modification|
|covalent chromatin modification|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|negative regulation of immune system process|
|regulation of hemopoiesis|
|mRNA processing|
|transcription by RNA polymerase II|
|centrosome|
|embryonic morphogenesis|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|protein ubiquitination|
|mRNA metabolic process|
|chromatin organization|
|negative regulation of cell differentiation|
|protein modification by small protein conjugation|
|RNA processing|
|anatomical structure formation involved in morphogenesis|
|negative regulation of developmental process|
|embryo development|
|protein modification by small protein conjugation or removal|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|cell-cell signaling|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of multicellular organismal process|
|positive regulation of transcription by RNA polymerase II|
|organic cyclic compound biosynthetic process|
|positive regulation of transcription, DNA-templated|
|protein-containing complex assembly|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of immune system process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of RNA metabolic process|
|tissue development|
|macromolecule biosynthetic process|
|regulation of cell differentiation|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|-2.91|
|[[:results:exp426|FBS-Wisent 0.1 R07 exp426]]|-2.55|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|-2.31|
|[[:results:exp458|Bisphenol S 100μM R08 exp458]]|-2.23|
|[[:results:exp332|Adefovir 20μM R07 exp332]]|-2.21|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-2.14|
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|-2.07|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|-2.03|
|[[:results:exp236|GSK2606414 1μM R05 exp236]]|-2.03|
|[[:results:exp140|Nicotinate 1000μM R03 exp140]]|-2.02|
|[[:results:exp347|Cyclosporin-A 0.8μM R07 exp347]]|-1.97|
|[[:results:exp496|Lead acetate 990μM R08 exp496]]|-1.96|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-1.92|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|-1.89|
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|-1.83|
|[[:results:exp359|FK-506 30μM R07 exp359]]|-1.82|
|[[:results:exp277|Curcumin 6.5μM R06 exp277]]|-1.81|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-1.8|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|-1.77|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|-1.76|
|[[:results:exp211|AICAR 240μM R05 exp211]]|-1.72|
|[[:results:exp165|RO-3306 3 to 4μM on day4 R04 exp165]]|-1.72|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|1.78|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|1.78|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|2.24|
|[[:results:exp534|Trientine 500μM R08 exp534]]|2.5|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 6065
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.9 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='LEO1 Expression in NALM6 Cells: 5.9'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>