======= MASTL =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MASTL
  * **<color #00a2e8>Official Name</color>**: microtubule associated serine/threonine kinase like
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=84930|84930]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96GX5|Q96GX5]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MASTL&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MASTL|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608221|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. {ECO:0000269|PubMed:12890928, ECO:0000269|PubMed:19680222, ECO:0000269|PubMed:19793917, ECO:0000269|PubMed:20538976, ECO:0000269|PubMed:20818157}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase|
|Pkinase Tyr|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|female meiosis II|
|meiosis II cell cycle process|
|meiosis II|
|positive regulation of ubiquitin protein ligase activity|
|regulation of ubiquitin protein ligase activity|
|female meiotic nuclear division|
|positive regulation of ubiquitin-protein transferase activity|
|protein phosphatase 2A binding|
|regulation of ubiquitin-protein transferase activity|
|cleavage furrow|
|negative regulation of phosphoprotein phosphatase activity|
|negative regulation of protein dephosphorylation|
|kinase activity|
|negative regulation of phosphatase activity|
|negative regulation of dephosphorylation|
|positive regulation of protein ubiquitination|
|regulation of phosphoprotein phosphatase activity|
|female gamete generation|
|G2/M transition of mitotic cell cycle|
|cell cycle G2/M phase transition|
|positive regulation of protein modification by small protein conjugation or removal|
|regulation of protein dephosphorylation|
|meiotic nuclear division|
|meiotic cell cycle process|
|peptidyl-serine phosphorylation|
|regulation of phosphatase activity|
|peptidyl-serine modification|
|regulation of protein ubiquitination|
|regulation of dephosphorylation|
|meiotic cell cycle|
|regulation of protein modification by small protein conjugation or removal|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|nuclear division|
|organelle fission|
|cellular process involved in reproduction in multicellular organism|
|protein serine/threonine kinase activity|
|negative regulation of hydrolase activity|
|centrosome|
|cell division|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|negative regulation of protein modification process|
|mitotic cell cycle process|
|positive regulation of transferase activity|
|mitotic cell cycle|
|gamete generation|
|cellular response to DNA damage stimulus|
|negative regulation of catalytic activity|
|multicellular organismal reproductive process|
|sexual reproduction|
|multicellular organism reproduction|
|peptidyl-amino acid modification|
|protein phosphorylation|
|regulation of transferase activity|
|multi-organism reproductive process|
|cell cycle process|
|negative regulation of cellular protein metabolic process|
|negative regulation of protein metabolic process|
|negative regulation of molecular function|
|regulation of cell cycle|
|positive regulation of protein modification process|
|regulation of hydrolase activity|
|phosphorylation|
|cell cycle|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|ATP binding|
|positive regulation of cellular protein metabolic process|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of protein modification process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|-2.19|
|[[:results:exp17|DABN 20μM R00 exp17]]|-2.1|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|-1.99|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|-1.93|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|-1.83|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-1.83|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|-1.77|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|-1.73|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-1.7|
|[[:results:exp300|VE-822 0.04μM R06 exp300]]|1.73|
|[[:results:exp71|KU-0063794 3.8μM R02 exp71]]|2.06|
|[[:results:exp149|SB203580 25μM R03 exp149]]|2.1|
|[[:results:exp114|A-196 10μM R03 exp114]]|2.12|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tspy2|TSPY2]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 726/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|26/26|
|breast|31/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|21/21|
|liver|19/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|22/26|
|pancreas|23/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|0/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|28/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1217
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.58 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MASTL Expression in NALM6 Cells: 6.58'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>