======= METTL14 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: METTL14
  * **<color #00a2e8>Official Name</color>**: methyltransferase like 14
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=57721|57721]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9HCE5|Q9HCE5]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=METTL14&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20METTL14|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/616504|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  The METTL3-METTL14 heterodimer forms a N6- methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6- methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity). {ECO:0000250|UniProtKB:Q3UIK4, ECO:0000269|PubMed:24316715, ECO:0000269|PubMed:24407421, ECO:0000269|PubMed:25719671, ECO:0000269|PubMed:27281194, ECO:0000269|PubMed:27373337, ECO:0000269|PubMed:27627798}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|MT-A70|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|mRNA (2-O-methyladenosine-N6-)-methyltransferase activity|
|forebrain radial glial cell differentiation|
|RNA N6-methyladenosine methyltransferase complex|
|S-adenosyl-L-methionine binding|
|radial glial cell differentiation|
|mRNA methylation|
|mRNA modification|
|mRNA destabilization|
|RNA destabilization|
|positive regulation of mRNA catabolic process|
|forebrain generation of neurons|
|positive regulation of mRNA metabolic process|
|RNA methylation|
|negative regulation of translation|
|stem cell population maintenance|
|maintenance of cell number|
|negative regulation of cellular amide metabolic process|
|mRNA binding|
|RNA modification|
|glial cell differentiation|
|regulation of mRNA stability|
|regulation of RNA stability|
|regulation of mRNA catabolic process|
|gliogenesis|
|mRNA catabolic process|
|RNA catabolic process|
|macromolecule methylation|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|RNA splicing, via transesterification reactions|
|methylation|
|regulation of mRNA metabolic process|
|regulation of translation|
|positive regulation of cellular catabolic process|
|nucleobase-containing compound catabolic process|
|forebrain development|
|RNA splicing|
|regulation of cellular amide metabolic process|
|positive regulation of catabolic process|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|mRNA processing|
|organic cyclic compound catabolic process|
|posttranscriptional regulation of gene expression|
|spermatogenesis|
|male gamete generation|
|gamete generation|
|mRNA metabolic process|
|brain development|
|head development|
|multicellular organismal reproductive process|
|sexual reproduction|
|regulation of cellular catabolic process|
|multicellular organism reproduction|
|RNA processing|
|cellular macromolecule catabolic process|
|central nervous system development|
|regulation of catabolic process|
|multi-organism reproductive process|
|negative regulation of cellular protein metabolic process|
|macromolecule catabolic process|
|negative regulation of protein metabolic process|
|negative regulation of cellular macromolecule biosynthetic process|
|reproductive process|
|reproduction|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|negative regulation of biosynthetic process|
|neurogenesis|
|RNA metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|-2.57|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-2.15|
|[[:results:exp247|UM0130462 0.025 to 0.035μM day4 R05 exp247]]|-2.14|
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|-2.06|
|[[:results:exp151|SGC0946 7μM R03 exp151]]|-1.92|
|[[:results:exp169|BH1 1μM R04 exp169]]|-1.89|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|-1.85|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-1.82|
|[[:results:exp494|Isoniazid 100μM R08 exp494]]|-1.81|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-1.73|
|[[:results:exp235|Geldanamycin 0.01μM R05 exp235]]|-1.72|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|1.76|
|[[:results:exp504|MK2206 4μM R08 exp504]]|1.77|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|1.77|
|[[:results:exp116|AICAR 240μM R03 exp116]]|1.81|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.93|
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|2.17|
|[[:results:exp458|Bisphenol S 100μM R08 exp458]]|2.27|
|[[:results:exp274|Citral 50μM R06 exp274]]|2.3|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|2.42|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|2.57|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mettl3|METTL3]]|0.494|
|[[:human genes:c:cbll1|CBLL1]]|0.475|
|[[:human genes:g:gemin8|GEMIN8]]|0.467|
|[[:human genes:c:cxorf56|CXorf56]]|0.457|
|[[:human genes:p:ptar1|PTAR1]]|0.441|
|[[:human genes:d:dph1|DPH1]]|0.439|
|[[:human genes:i:itpk1|ITPK1]]|0.436|
|[[:human genes:r:rpp21|RPP21]]|0.429|
|[[:human genes:r:rabggtb|RABGGTB]]|0.418|
|[[:human genes:y:ythdf2|YTHDF2]]|0.409|
|[[:human genes:g:gemin7|GEMIN7]]|0.407|
|[[:human genes:r:rbm26|RBM26]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 397/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|21/28|
|blood|12/28|
|bone|18/26|
|breast|17/33|
|central nervous system|33/56|
|cervix|3/4|
|colorectal|10/17|
|esophagus|8/13|
|fibroblast|0/1|
|gastric|9/16|
|kidney|11/21|
|liver|11/20|
|lung|39/75|
|lymphocyte|6/16|
|ovary|14/26|
|pancreas|17/24|
|peripheral nervous system|9/16|
|plasma cell|8/15|
|prostate|0/1|
|skin|5/24|
|soft tissue|3/9|
|thyroid|2/2|
|upper aerodigestive|15/22|
|urinary tract|22/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1989
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.55 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='METTL14 Expression in NALM6 Cells: 5.55'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>