======= NCBP1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NCBP1
  * **<color #00a2e8>Official Name</color>**: nuclear cap binding protein subunit 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4686|4686]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q09161|Q09161]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NCBP1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NCBP1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600469|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap- binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858). {ECO:0000269|PubMed:11551508, ECO:0000269|PubMed:12093754, ECO:0000269|PubMed:15059963, ECO:0000269|PubMed:15361857, ECO:0000269|PubMed:16186820, ECO:0000269|PubMed:16317009, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:17873884, ECO:0000269|PubMed:18369367, ECO:0000269|PubMed:19632182, ECO:0000269|PubMed:19648179, ECO:0000269|PubMed:26382858, ECO:0000269|PubMed:7651522, ECO:0000269|PubMed:8069914}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|MIF4G|
|MIF4G like|
|MIF4G like 2|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|RNA cap binding complex|
|nuclear cap binding complex|
|positive regulation of RNA binding|
|regulation of RNA binding|
|positive regulation of mRNA 3-end processing|
|RNA cap binding|
|pre-mRNA cleavage required for polyadenylation|
|mRNA cap binding complex|
|mRNA cleavage involved in mRNA processing|
|RNA 7-methylguanosine cap binding|
|regulation of mRNA polyadenylation|
|positive regulation of mRNA splicing, via spliceosome|
|mRNA cleavage|
|regulation of mRNA 3-end processing|
|histone mRNA metabolic process|
|positive regulation of mRNA processing|
|7-methylguanosine mRNA capping|
|mRNA cis splicing, via spliceosome|
|termination of RNA polymerase II transcription|
|positive regulation of RNA splicing|
|RNA capping|
|7-methylguanosine RNA capping|
|spliceosomal complex assembly|
|snRNA transcription by RNA polymerase II|
|snRNA transcription|
|DNA-templated transcription, termination|
|transcription elongation from RNA polymerase II promoter|
|positive regulation of mRNA metabolic process|
|regulation of translational initiation|
|fibroblast growth factor receptor signaling pathway|
|mRNA 3-end processing|
|gene silencing by RNA|
|DNA-templated transcription, elongation|
|ncRNA transcription|
|regulation of mRNA splicing, via spliceosome|
|mRNA export from nucleus|
|cellular response to fibroblast growth factor stimulus|
|mRNA-containing ribonucleoprotein complex export from nucleus|
|response to fibroblast growth factor|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|ribonucleoprotein complex export from nucleus|
|ribonucleoprotein complex localization|
|RNA export from nucleus|
|RNA 3-end processing|
|regulation of RNA splicing|
|regulation of mRNA processing|
|protein export from nucleus|
|gene silencing|
|mRNA transport|
|ribonucleoprotein complex|
|nuclear export|
|RNA phosphodiester bond hydrolysis|
|positive regulation of cell growth|
|positive regulation of binding|
|RNA transport|
|nucleic acid transport|
|establishment of RNA localization|
|nuclear-transcribed mRNA catabolic process|
|RNA localization|
|mRNA catabolic process|
|ribonucleoprotein complex assembly|
|nucleobase-containing compound transport|
|ribonucleoprotein complex subunit organization|
|RNA catabolic process|
|protein-containing complex localization|
|positive regulation of growth|
|nucleocytoplasmic transport|
|nuclear transport|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|mRNA splicing, via spliceosome|
|nucleic acid phosphodiester bond hydrolysis|
|RNA splicing, via transesterification reactions|
|regulation of mRNA metabolic process|
|regulation of translation|
|nucleobase-containing compound catabolic process|
|regulation of binding|
|RNA splicing|
|regulation of cellular amide metabolic process|
|regulation of cell growth|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|ribonucleoprotein complex biogenesis|
|mRNA processing|
|organic cyclic compound catabolic process|
|transcription by RNA polymerase II|
|cellular response to growth factor stimulus|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|posttranscriptional regulation of gene expression|
|response to growth factor|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|regulation of growth|
|mRNA metabolic process|
|enzyme linked receptor protein signaling pathway|
|cellular protein-containing complex assembly|
|RNA processing|
|cellular macromolecule catabolic process|
|intracellular protein transport|
|macromolecule catabolic process|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|cellular response to endogenous stimulus|
|mitochondrion|
|organic cyclic compound biosynthetic process|
|RNA binding|
|response to endogenous stimulus|
|protein transport|
|intracellular transport|
|peptide transport|
|protein-containing complex assembly|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|positive regulation of molecular function|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of gene expression|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp123|GSK-LSD1 10μM R03 exp123]]|-2.03|
|[[:results:exp26|Oligomycin-A 20μM R00 exp26]]|-1.92|
|[[:results:exp225|Celastrol 0.12μM R05 exp225]]|-1.88|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|-1.87|
|[[:results:exp494|Isoniazid 100μM R08 exp494]]|-1.86|
|[[:results:exp211|AICAR 240μM R05 exp211]]|-1.79|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-1.76|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|-1.71|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|1.7|
|[[:results:exp504|MK2206 4μM R08 exp504]]|1.72|
|[[:results:exp291|LLY-284 2.6μM R06 exp291]]|1.73|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|1.84|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|1.87|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|1.9|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|1.92|
|[[:results:exp417|Tubastatin-A 2.5μM R07 exp417]]|1.93|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|1.94|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|2.08|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|2.08|
|[[:results:exp455|Benzoate 10000μM R08 exp455]]|2.17|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|2.36|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|2.44|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|2.45|
|[[:results:exp453|B02 10μM R08 exp453]]|2.53|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|2.54|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:med14|MED14]]|0.419|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.405|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 680/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|27/28|
|bone|24/25|
|breast|31/33|
|central nervous system|50/56|
|cervix|4/4|
|colorectal|16/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|18/21|
|liver|19/20|
|lung|70/75|
|lymphocyte|14/14|
|ovary|25/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|22/24|
|soft tissue|5/7|
|thyroid|2/2|
|upper aerodigestive|15/22|
|urinary tract|28/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 838
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.14 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NCBP1 Expression in NALM6 Cells: 7.14'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>