======= NEK2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NEK2
  * **<color #00a2e8>Official Name</color>**: NIMA related kinase 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4751|4751]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P51955|P51955]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NEK2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NEK2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/604043|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856). {ECO:0000269|PubMed:11742531, ECO:0000269|PubMed:12857871, ECO:0000269|PubMed:14978040, ECO:0000269|PubMed:15358203, ECO:0000269|PubMed:15388344, ECO:0000269|PubMed:17283141, ECO:0000269|PubMed:17621308, ECO:0000269|PubMed:17626005, ECO:0000269|PubMed:18086858, ECO:0000269|PubMed:18297113, ECO:0000269|PubMed:20034488, ECO:0000269|PubMed:21076410, ECO:0000269|PubMed:24554434, ECO:0000269|PubMed:25704143, ECO:0000269|PubMed:26220856}. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S- arrested cells. {ECO:0000269|PubMed:15161910}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase Tyr|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of centriole-centriole cohesion|
|regulation of centriole-centriole cohesion|
|centrosome separation|
|regulation of mitotic centrosome separation|
|regulation of attachment of spindle microtubules to kinetochore|
|negative regulation of centrosome cycle|
|positive regulation of telomere capping|
|regulation of telomere capping|
|condensed nuclear chromosome|
|positive regulation of telomere maintenance via telomerase|
|positive regulation of telomerase activity|
|mitotic spindle assembly|
|positive regulation of telomere maintenance via telomere lengthening|
|positive regulation of telomere maintenance|
|regulation of telomerase activity|
|regulation of telomere maintenance via telomerase|
|negative regulation of DNA binding|
|regulation of centrosome cycle|
|regulation of telomere maintenance via telomere lengthening|
|positive regulation of DNA biosynthetic process|
|mitotic spindle organization|
|centrosome cycle|
|regulation of telomere maintenance|
|protein phosphatase binding|
|kinetochore|
|condensed chromosome kinetochore|
|microtubule organizing center organization|
|ciliary basal body-plasma membrane docking|
|blastocyst development|
|spindle assembly|
|regulation of chromosome segregation|
|microtubule cytoskeleton organization involved in mitosis|
|mitotic sister chromatid segregation|
|regulation of DNA biosynthetic process|
|spindle pole|
|regulation of DNA binding|
|G2/M transition of mitotic cell cycle|
|cell cycle G2/M phase transition|
|negative regulation of cytoskeleton organization|
|sister chromatid segregation|
|mitotic nuclear division|
|spindle organization|
|midbody|
|regulation of mitotic nuclear division|
|negative regulation of binding|
|organelle localization by membrane tethering|
|positive regulation of chromosome organization|
|membrane docking|
|regulation of microtubule cytoskeleton organization|
|regulation of nuclear division|
|positive regulation of DNA metabolic process|
|protein autophosphorylation|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of cell cycle G2/M phase transition|
|nuclear chromosome segregation|
|regulation of microtubule-based process|
|meiotic cell cycle|
|protein kinase activity|
|mitotic cell cycle phase transition|
|chromosome segregation|
|cell cycle phase transition|
|nuclear division|
|organelle fission|
|microtubule|
|negative regulation of cell cycle process|
|cilium assembly|
|regulation of chromosome organization|
|regulation of DNA metabolic process|
|protein serine/threonine kinase activity|
|cilium organization|
|negative regulation of organelle organization|
|in utero embryonic development|
|regulation of binding|
|regulation of mitotic cell cycle phase transition|
|plasma membrane bounded cell projection assembly|
|regulation of cell cycle phase transition|
|cell projection assembly|
|microtubule cytoskeleton organization|
|centrosome|
|cell division|
|regulation of cytoskeleton organization|
|negative regulation of cell cycle|
|organelle localization|
|protein-containing complex|
|mitotic cell cycle process|
|positive regulation of organelle organization|
|regulation of mitotic cell cycle|
|chordate embryonic development|
|embryo development ending in birth or egg hatching|
|positive regulation of transferase activity|
|microtubule-based process|
|mitotic cell cycle|
|negative regulation of cellular component organization|
|regulation of cell cycle process|
|organelle assembly|
|nucleolus|
|protein phosphorylation|
|embryo development|
|regulation of transferase activity|
|cell cycle process|
|chromosome organization|
|cytoskeleton organization|
|plasma membrane bounded cell projection organization|
|negative regulation of molecular function|
|cell projection organization|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|phosphorylation|
|regulation of organelle organization|
|cell cycle|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|ATP binding|
|positive regulation of molecular function|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-2.01|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|1.79|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 7708
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.21 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NEK2 Expression in NALM6 Cells: 5.21'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>