======= OBFC1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: STN1
  * **<color #00a2e8>Official Name</color>**: STN1 subunit of CST complex
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=79991|79991]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9H668|Q9H668]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=OBFC1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20OBFC1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/613128|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22964711, PubMed:22763445). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re- initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097). Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres (PubMed:22863775, PubMed:22964711). Promotes general replication start following replication-fork stalling implicating new origin firing (PubMed:22863775). May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication (PubMed:23142664). {ECO:0000269|PubMed:19648609, ECO:0000269|PubMed:19854130, ECO:0000269|PubMed:22763445, ECO:0000269|PubMed:22863775, ECO:0000269|PubMed:22964711, ECO:0000269|PubMed:23142664, ECO:0000269|PubMed:25483097, ECO:0000305|PubMed:23851344}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DUF1879|
|tRNA anti|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|CST complex|
|single-stranded telomeric DNA binding|
|telomere capping|
|negative regulation of telomere maintenance via telomerase|
|negative regulation of telomere maintenance via telomere lengthening|
|telomeric DNA binding|
|telomere maintenance via telomere lengthening|
|positive regulation of DNA replication|
|negative regulation of telomere maintenance|
|negative regulation of DNA biosynthetic process|
|intermediate filament cytoskeleton|
|regulation of telomere maintenance via telomerase|
|regulation of telomere maintenance via telomere lengthening|
|regulation of telomere maintenance|
|telomere maintenance|
|telomere organization|
|single-stranded DNA binding|
|regulation of DNA replication|
|nuclear chromosome, telomeric region|
|regulation of DNA biosynthetic process|
|negative regulation of DNA metabolic process|
|fibrillar center|
|negative regulation of chromosome organization|
|anatomical structure homeostasis|
|regulation of chromosome organization|
|regulation of DNA metabolic process|
|negative regulation of organelle organization|
|intracellular membrane-bounded organelle|
|negative regulation of cellular component organization|
|DNA metabolic process|
|chromosome organization|
|regulation of organelle organization|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|homeostatic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.93|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|-1.91|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|-1.83|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:ten1|TEN1]]|0.539|
|[[:human genes:c:ctc1|CTC1]]|0.477|
|[[:human genes:t:thg1l|THG1L]]|0.428|
|[[:human genes:e:eif3f|EIF3F]]|0.427|
|[[:human genes:g:gmppb|GMPPB]]|0.407|
|[[:human genes:u:uap1|UAP1]]|0.403|
|[[:human genes:t:trmt61a|TRMT61A]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 45/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|1/28|
|blood|4/28|
|bone|2/26|
|breast|4/33|
|central nervous system|5/56|
|cervix|0/4|
|colorectal|2/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|1/20|
|lung|5/75|
|lymphocyte|1/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|4/22|
|urinary tract|2/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1557
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 3.34 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='OBFC1 Expression in NALM6 Cells: 3.34'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>