======= OTUB1 ======= == Gene Information == * **Official Symbol**: OTUB1 * **Official Name**: OTU deubiquitinase, ubiquitin aldehyde binding 1 * **Aliases and Previous Symbols**: N/A * **Entrez ID**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55611|55611]] * **UniProt**: [[https://www.uniprot.org/uniprot/Q96FW1|Q96FW1]] * **Interactions**: [[https://thebiogrid.org/search.php?search=OTUB1&organism=9606|BioGRID]] * **PubMed articles**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20OTUB1|Open PubMed]] * **OMIM**: [[https://omim.org/entry/608337|Open OMIM]] == Function Summary == * **Entrez Summary**: N/A * **UniProt Summary**: Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys- 48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin. |Peptidase C65| |regulation of histone H2A K63-linked ubiquitination| |negative regulation of histone ubiquitination| |negative regulation of histone H2A K63-linked ubiquitination| |negative regulation of protein K63-linked ubiquitination| |NEDD8-specific protease activity| |negative regulation of protein polyubiquitination| |regulation of protein K63-linked ubiquitination| |regulation of histone ubiquitination| |regulation of protein polyubiquitination| |negative regulation of double-strand break repair| |protein K48-linked deubiquitination| |negative regulation of DNA repair| |negative regulation of histone modification| |negative regulation of chromatin organization| |negative regulation of protein ubiquitination| |regulation of double-strand break repair| |ubiquitin binding| |negative regulation of response to DNA damage stimulus| |negative regulation of protein modification by small protein conjugation or removal| |thiol-dependent ubiquitin-specific protease activity| |negative regulation of DNA metabolic process| |regulation of DNA repair| |negative regulation of chromosome organization| |regulation of histone modification| |cellular response to interleukin-1| |regulation of chromatin organization| |response to interleukin-1| |regulation of protein ubiquitination| |regulation of response to DNA damage stimulus| |regulation of protein modification by small protein conjugation or removal| |protein deubiquitination| |ubiquitin protein ligase binding| |protein modification by small protein removal| |regulation of chromosome organization| |regulation of DNA metabolic process| |negative regulation of organelle organization| |DNA repair| |negative regulation of protein modification process| |adaptive immune response| |negative regulation of cellular component organization| |regulation of cellular response to stress| |DNA metabolic process| |cellular response to DNA damage stimulus| |protein modification by small protein conjugation or removal| |cellular response to cytokine stimulus| |negative regulation of cellular protein metabolic process| |response to cytokine| |negative regulation of protein metabolic process| |proteolysis| |regulation of organelle organization| |negative regulation of nucleobase-containing compound metabolic process| |regulation of response to stress| |negative regulation of response to stimulus| |cellular response to stress| |regulation of protein modification process| |immune response| \\ === CRISPR Data === ^Screen^Score^ |[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|-4.24| |[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|-3.78| |[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|-3.69| |[[:results:exp442|Ibrutinib 10μM R08 exp442]]|-3.61| |[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-3.58| |[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|-3.45| |[[:results:exp499|LY2090314 0.003μM R08 exp499]]|-3.37| |[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-3.27| |[[:results:exp67|BVD-523 15μM R02 exp67]]|-3.23| |[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-3.14| |[[:results:exp85|UM0129480 7μM R02 exp85]]|-2.99| |[[:results:exp269|Bisphenol A 100μM R06 exp269]]|-2.98| |[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-2.84| |[[:results:exp189|Temozolomide 200μM R04 exp189]]|-2.83| |[[:results:exp435|JQ1 0.8μM R08 exp435]]|-2.76| |[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|-2.75| |[[:results:exp485|GSK626616 14μM R08 exp485]]|-2.74| |[[:results:exp532|TIC10 10μM R08 exp532]]|-2.73| |[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2.7| |[[:results:exp527|Tanespimycin 14μM R08 exp527]]|-2.66| |[[:results:exp439|QNZ 0.01μM R08 exp439]]|-2.59| |[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|-2.58| |[[:results:exp420|Tunicamycin 0.04 to 0.125μM on day4 R07 exp420]]|-2.58| |[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|-2.57| |[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|-2.55| |[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-2.54| |[[:results:exp513|ONC212 0.15μM R08 exp513]]|-2.53| |[[:results:exp502|Milciclib 2μM R08 exp502]]|-2.5| |[[:results:exp343|Centrinone 0.5μM R07 exp343]]|-2.46| |[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|-2.45| |[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2.44| |[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|-2.41| |[[:results:exp474|CR131-b 0.005μM R08 exp474]]|-2.41| |[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.41| |[[:results:exp147|Resveratrol 16μM R03 exp147]]|-2.4| |[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-2.37| |[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|-2.36| |[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|-2.3| |[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|-2.29| |[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|-2.27| |[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-2.26| |[[:results:exp72|LB-100 4.1μM R02 exp72]]|-2.24| |[[:results:exp475|CyclicAMP 200μM R08 exp475]]|-2.22| |[[:results:exp433|LJH685 50μM R08 exp433]]|-2.22| |[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|-2.2| |[[:results:exp450|Artemisinin 50μM R08 exp450]]|-2.18| |[[:results:exp515|PU-H71 1μM R08 exp515]]|-2.17| |[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-2.15| |[[:results:exp492|iCRT14 30μM R08 exp492]]|-2.14| |[[:results:exp217|Mdivi-1 15μM R05 exp217]]|-2.13| |[[:results:exp497|Lead acetate 2000μM R08 exp497]]|-2.11| |[[:results:exp359|FK-506 30μM R07 exp359]]|-2.08| |[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.08| |[[:results:exp453|B02 10μM R08 exp453]]|-2.05| |[[:results:exp512|Olaparib 4μM R08 exp512]]|-2.05| |[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-2.03| |[[:results:exp505|ML-792 0.2μM R08 exp505]]|-2.02| |[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|-2.02| |[[:results:exp59|UMK57 1μM R01 exp59]]|-2| |[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-1.97| |[[:results:exp234|Ethanol 0.01 R05 exp234]]|-1.9| |[[:results:exp507|Monensin 0.3μM R08 exp507]]|-1.89| |[[:results:exp466|Cannabidiol 20μM R08 exp466]]|-1.83| |[[:results:exp187|proTAME 5μM R04 exp187]]|-1.83| |[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|-1.81| |[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311]]|-1.79| |[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|-1.79| |[[:results:exp443|SNS-032 15μM R08 exp443]]|-1.79| |[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-1.78| |[[:results:exp216|Erlotinib 10μM R05 exp216]]|-1.78| |[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|-1.76| |[[:results:exp431|Rotenone 0.07μM R08 exp431]]|-1.75| |[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|-1.75| |[[:results:exp472|CI-1040 9.5μM R08 exp472]]|-1.74| |[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-1.74| |[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|-1.73| |[[:results:exp82|Torin1 0.08μM R02 exp82]]|-1.73| |[[:results:exp495|IWR1 50μM R08 exp495]]|-1.72| |[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|-1.71| |[[:results:exp469|CFI-400945 25μM R08 exp469]]|-1.7| |[[:results:exp273|Cisplatin 0.35μM R06 exp273]]|-1.7| |[[:results:exp413|THZ531 0.11 to 0.175μM on day4 R07 exp413]]|1.78| |[[:results:exp423|Zebularine 20μM R07 exp423]]|1.78| |[[:results:exp18|Doxycycline 10μM R00 exp18]]|1.79| |[[:results:exp409|THZ531 0.11μM R07 exp409]]|1.82| |[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|2.99| |[[:results:exp29|Rapamycin 1μM R00 exp29]]|3.34| ^Gene^Correlation^ |[[:human genes:u:ube2d3|UBE2D3]]|0.484| |[[:human genes:y:ypel5|YPEL5]]|0.479| |[[:human genes:u:ube2d2|UBE2D2]]|0.473| |[[:human genes:r:ranbp1|RANBP1]]|0.47| |[[:human genes:w:wdr26|WDR26]]|0.466| |[[:human genes:u:ube2h|UBE2H]]|0.464| |[[:human genes:p:pcgf1|PCGF1]]|0.437| |[[:human genes:n:nup188|NUP188]]|0.433| |[[:human genes:c:c19orf53|C19orf53]]|0.431| |[[:human genes:c:cnot8|CNOT8]]|0.425| |[[:human genes:r:ring1|RING1]]|0.416| |[[:human genes:u:ubb|UBB]]|0.415| Global Fraction of Cell Lines Where Essential: 0/726 ^Tissue^Fraction Of Cell Lines Where Essential^ |1290807.0|0/1| |909776.0|0/1| |bile duct|0/28| |blood|0/28| |bone|0/25| |breast|0/33| |central nervous system|0/56| |cervix|0/4| |colorectal|0/17| |esophagus|0/13| |fibroblast|0/1| |gastric|0/15| |kidney|0/21| |liver|0/20| |lung|0/75| |lymphocyte|0/14| |ovary|0/26| |pancreas|0/24| |peripheral nervous system|0/16| |plasma cell|0/15| |prostate|0/1| |skin|0/24| |soft tissue|0/7| |thyroid|0/2| |upper aerodigestive|0/22| |urinary tract|0/29| |uterus|0/5| == Essentiality in NALM6 == * **Essentiality Rank**: 4538 * **Expression level (log2 read counts)**: 6.04 {{:chemogenomics:nalm6 dist.png?nolink |}}