======= PIF1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PIF1
  * **<color #00a2e8>Official Name</color>**: PIF1 5'-to-3' DNA helicase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=80119|80119]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9H611|Q9H611]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PIF1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PIF1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610953|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. {ECO:0000255|HAMAP-Rule:MF_03176, ECO:0000269|PubMed:16522649, ECO:0000269|PubMed:17172855, ECO:0000269|PubMed:17827721, ECO:0000269|PubMed:18835853, ECO:0000269|PubMed:19700773, ECO:0000269|PubMed:20524933, ECO:0000269|PubMed:23657261}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PIF1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|5-3 DNA/RNA helicase activity|
|telomerase inhibitor activity|
|5-3 DNA helicase activity|
|G-quadruplex DNA binding|
|negative regulation of telomerase activity|
|single-stranded DNA helicase activity|
|mitochondrial genome maintenance|
|negative regulation of telomere maintenance via telomerase|
|replication fork|
|negative regulation of telomere maintenance via telomere lengthening|
|telomeric DNA binding|
|negative regulation of telomere maintenance|
|negative regulation of DNA biosynthetic process|
|regulation of telomerase activity|
|regulation of telomere maintenance via telomerase|
|regulation of telomere maintenance via telomere lengthening|
|regulation of telomere maintenance|
|telomere maintenance|
|telomere organization|
|nuclear chromosome, telomeric region|
|DNA duplex unwinding|
|regulation of DNA biosynthetic process|
|DNA geometric change|
|negative regulation of DNA metabolic process|
|negative regulation of chromosome organization|
|DNA replication|
|magnesium ion binding|
|DNA recombination|
|negative regulation of transferase activity|
|DNA conformation change|
|anatomical structure homeostasis|
|regulation of chromosome organization|
|regulation of DNA metabolic process|
|negative regulation of organelle organization|
|mitochondrion organization|
|DNA repair|
|negative regulation of cellular component organization|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|negative regulation of catalytic activity|
|regulation of transferase activity|
|chromosome organization|
|negative regulation of molecular function|
|mitochondrion|
|regulation of organelle organization|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|ATP binding|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|-2|
|[[:results:exp345|Cidofovir 10μM R07 exp345]]|1.82|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:prim1|PRIM1]]|0.429|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 3524
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.43 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PIF1 Expression in NALM6 Cells: 6.43'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>