======= PNPT1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PNPT1
  * **<color #00a2e8>Official Name</color>**: polyribonucleotide nucleotidyltransferase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=87178|87178]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8TCS8|Q8TCS8]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PNPT1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PNPT1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610316|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012]. 
  * **<color #00a2e8>UniProt Summary</color>**:  RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double- stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. {ECO:0000269|PubMed:12473748, ECO:0000269|PubMed:12721301, ECO:0000269|PubMed:12798676, ECO:0000269|PubMed:16055741, ECO:0000269|PubMed:16410805, ECO:0000269|PubMed:16934922, ECO:0000269|PubMed:18083836, ECO:0000269|PubMed:18083837, ECO:0000269|PubMed:18501193, ECO:0000269|PubMed:19509288, ECO:0000269|PubMed:20547861, ECO:0000269|PubMed:20691904}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|KH 1|
|S1|
|RNase PH|
|PNPase|
|RNase PH C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|mitochondrial mRNA 3-end processing|
|mitochondrial mRNA polyadenylation|
|polyribonucleotide nucleotidyltransferase activity|
|positive regulation of mitochondrial RNA catabolic process|
|positive regulation of miRNA catabolic process|
|mitochondrial mRNA processing|
|mitochondrial degradosome|
|mitochondrial RNA catabolic process|
|regulation of miRNA catabolic process|
|rRNA import into mitochondrion|
|mitochondrial mRNA catabolic process|
|mitochondrial RNA 5-end processing|
|regulation of mitochondrial RNA catabolic process|
|polyadenylation-dependent mRNA catabolic process|
|nuclear polyadenylation-dependent mRNA catabolic process|
|rRNA transport|
|positive regulation of miRNA metabolic process|
|RNA import into mitochondrion|
|mitochondrial RNA 3-end processing|
|poly(G) binding|
|polyadenylation-dependent RNA catabolic process|
|regulation of miRNA metabolic process|
|mitochondrial RNA processing|
|mitotic cell cycle arrest|
|cellular response to interferon-beta|
|RNA 5-end processing|
|mitochondrion morphogenesis|
|response to interferon-beta|
|mRNA polyadenylation|
|poly(U) RNA binding|
|regulation of cellular respiration|
|miRNA binding|
|RNA polyadenylation|
|liver regeneration|
|3-5-exoribonuclease activity|
|polysomal ribosome|
|protein homotrimerization|
|response to growth hormone|
|mitochondrial RNA metabolic process|
|regulation of cellular senescence|
|RNA phosphodiester bond hydrolysis, exonucleolytic|
|regulation of cell aging|
|positive regulation of mRNA catabolic process|
|protein trimerization|
|animal organ regeneration|
|mitochondrial intermembrane space|
|positive regulation of mRNA metabolic process|
|mRNA 3-end processing|
|mitochondrial transmembrane transport|
|response to cAMP|
|liver development|
|hepaticobiliary system development|
|RNA 3-end processing|
|response to organophosphorus|
|mitochondrial gene expression|
|cell cycle arrest|
|response to purine-containing compound|
|regeneration|
|regulation of generation of precursor metabolites and energy|
|RNA phosphodiester bond hydrolysis|
|RNA transport|
|nucleic acid transport|
|establishment of RNA localization|
|regulation of mRNA catabolic process|
|mitochondrial transport|
|RNA localization|
|mRNA catabolic process|
|nucleobase-containing compound transport|
|cellular response to oxidative stress|
|negative regulation of growth|
|RNA catabolic process|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|regulation of mRNA metabolic process|
|protein homooligomerization|
|positive regulation of cellular catabolic process|
|nucleobase-containing compound catabolic process|
|response to oxidative stress|
|response to peptide hormone|
|gland development|
|positive regulation of catabolic process|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|mitochondrion organization|
|aromatic compound catabolic process|
|response to peptide|
|mRNA processing|
|organic cyclic compound catabolic process|
|cell part morphogenesis|
|protein complex oligomerization|
|modification-dependent macromolecule catabolic process|
|negative regulation of cell cycle|
|regulation of mitotic cell cycle|
|regulation of growth|
|mRNA metabolic process|
|regulation of cellular response to stress|
|cellular component morphogenesis|
|regulation of cellular catabolic process|
|RNA processing|
|cellular macromolecule catabolic process|
|response to hormone|
|response to organic cyclic compound|
|endoplasmic reticulum membrane|
|regulation of catabolic process|
|cell cycle process|
|response to organonitrogen compound|
|cellular response to cytokine stimulus|
|macromolecule catabolic process|
|response to nitrogen compound|
|response to cytokine|
|regulation of cell cycle|
|mitochondrion|
|transmembrane transport|
|cell cycle|
|RNA binding|
|response to endogenous stimulus|
|regulation of response to stress|
|response to oxygen-containing compound|
|protein-containing complex assembly|
|RNA metabolic process|
|cellular response to stress|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|nitrogen compound transport|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|1.7|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|1.73|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|1.78|
|[[:results:exp532|TIC10 10μM R08 exp532]]|1.78|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|1.79|
|[[:results:exp271|CCT251545 0.2μM R06 exp271]]|1.81|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|1.83|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|1.83|
|[[:results:exp26|Oligomycin-A 20μM R00 exp26]]|1.9|
|[[:results:exp8|Brefeldin A 0.02μM R00 exp8]]|1.92|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|1.92|
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.93|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|2.27|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mrpl21|MRPL21]]|0.632|
|[[:human genes:t:tufm|TUFM]]|0.63|
|[[:human genes:m:mrps14|MRPS14]]|0.628|
|[[:human genes:m:mrp63|MRP63]]|0.611|
|[[:human genes:g:gfm1|GFM1]]|0.611|
|[[:human genes:t:tars2|TARS2]]|0.603|
|[[:human genes:m:mrps21|MRPS21]]|0.598|
|[[:human genes:m:mrpl47|MRPL47]]|0.595|
|[[:human genes:s:supv3l1|SUPV3L1]]|0.59|
|[[:human genes:y:yars2|YARS2]]|0.587|
|[[:human genes:h:hsd17b10|HSD17B10]]|0.587|
|[[:human genes:d:dap3|DAP3]]|0.587|
|[[:human genes:m:mrps6|MRPS6]]|0.583|
|[[:human genes:b:bcs1l|BCS1L]]|0.577|
|[[:human genes:k:kiaa0391|KIAA0391]]|0.577|
|[[:human genes:v:vars2|VARS2]]|0.574|
|[[:human genes:s:sars2|SARS2]]|0.574|
|[[:human genes:m:mrps34|MRPS34]]|0.573|
|[[:human genes:n:nars2|NARS2]]|0.571|
|[[:human genes:d:dars2|DARS2]]|0.57|
|[[:human genes:c:cox16|COX16]]|0.567|
|[[:human genes:s:selrc1|SELRC1]]|0.565|
|[[:human genes:r:rpusd4|RPUSD4]]|0.562|
|[[:human genes:t:tefm|TEFM]]|0.562|
|[[:human genes:m:mrpl53|MRPL53]]|0.56|
|[[:human genes:m:mrps26|MRPS26]]|0.558|
|[[:human genes:m:mrps16|MRPS16]]|0.557|
|[[:human genes:r:rars2|RARS2]]|0.551|
|[[:human genes:m:mars2|MARS2]]|0.549|
|[[:human genes:m:mrps5|MRPS5]]|0.547|
|[[:human genes:w:wbscr16|WBSCR16]]|0.546|
|[[:human genes:g:gtpbp10|GTPBP10]]|0.545|
|[[:human genes:s:sco2|SCO2]]|0.544|
|[[:human genes:q:qrsl1|QRSL1]]|0.541|
|[[:human genes:n:ndufa3|NDUFA3]]|0.541|
|[[:human genes:t:trub2|TRUB2]]|0.54|
|[[:human genes:m:mrps25|MRPS25]]|0.539|
|[[:human genes:m:mrpl20|MRPL20]]|0.535|
|[[:human genes:m:mrps9|MRPS9]]|0.535|
|[[:human genes:r:rbfa|RBFA]]|0.535|
|[[:human genes:p:pars2|PARS2]]|0.535|
|[[:human genes:n:ndufv1|NDUFV1]]|0.532|
|[[:human genes:f:ftsj2|FTSJ2]]|0.53|
|[[:human genes:n:ndufaf7|NDUFAF7]]|0.529|
|[[:human genes:c:c12orf65|C12orf65]]|0.529|
|[[:human genes:h:hars2|HARS2]]|0.527|
|[[:human genes:g:gfm2|GFM2]]|0.526|
|[[:human genes:s:slc25a26|SLC25A26]]|0.524|
|[[:human genes:m:mrpl23|MRPL23]]|0.524|
|[[:human genes:c:cars2|CARS2]]|0.521|
|[[:human genes:m:mrps23|MRPS23]]|0.52|
|[[:human genes:m:mrpl45|MRPL45]]|0.519|
|[[:human genes:m:mrpl15|MRPL15]]|0.518|
|[[:human genes:a:atp5a1|ATP5A1]]|0.516|
|[[:human genes:a:atp5d|ATP5D]]|0.515|
|[[:human genes:r:rpusd3|RPUSD3]]|0.513|
|[[:human genes:n:ndufb11|NDUFB11]]|0.513|
|[[:human genes:f:fastkd5|FASTKD5]]|0.511|
|[[:human genes:m:mtpap|MTPAP]]|0.507|
|[[:human genes:l:lars2|LARS2]]|0.504|
|[[:human genes:m:mrpl3|MRPL3]]|0.503|
|[[:human genes:n:ndufc2|NDUFC2]]|0.502|
|[[:human genes:m:mrps33|MRPS33]]|0.502|
|[[:human genes:a:atp5c1|ATP5C1]]|0.5|
|[[:human genes:m:mtrf1l|MTRF1L]]|0.5|
|[[:human genes:c:cox17|COX17]]|0.499|
|[[:human genes:c:coq2|COQ2]]|0.498|
|[[:human genes:c:cox7b|COX7B]]|0.495|
|[[:human genes:m:mrpl52|MRPL52]]|0.494|
|[[:human genes:t:tfam|TFAM]]|0.492|
|[[:human genes:c:cycs|CYCS]]|0.491|
|[[:human genes:m:mrps18a|MRPS18A]]|0.489|
|[[:human genes:e:ears2|EARS2]]|0.488|
|[[:human genes:n:ndufa1|NDUFA1]]|0.487|
|[[:human genes:t:tomm22|TOMM22]]|0.487|
|[[:human genes:m:mtg2|MTG2]]|0.483|
|[[:human genes:m:mrpl12|MRPL12]]|0.482|
|[[:human genes:m:mrpl34|MRPL34]]|0.482|
|[[:human genes:c:chchd1|CHCHD1]]|0.478|
|[[:human genes:m:mrpl42|MRPL42]]|0.476|
|[[:human genes:g:gatc|GATC]]|0.474|
|[[:human genes:c:cox15|COX15]]|0.474|
|[[:human genes:m:mrpl44|MRPL44]]|0.474|
|[[:human genes:f:fars2|FARS2]]|0.474|
|[[:human genes:m:mrps10|MRPS10]]|0.473|
|[[:human genes:h:hccs|HCCS]]|0.472|
|[[:human genes:p:pet112|PET112]]|0.471|
|[[:human genes:r:rnaseh1|RNASEH1]]|0.471|
|[[:human genes:a:atp5b|ATP5B]]|0.47|
|[[:human genes:m:mrps24|MRPS24]]|0.469|
|[[:human genes:m:mrpl39|MRPL39]]|0.469|
|[[:human genes:m:mrps11|MRPS11]]|0.468|
|[[:human genes:m:mrps12|MRPS12]]|0.468|
|[[:human genes:m:mrps2|MRPS2]]|0.466|
|[[:human genes:n:ndufs2|NDUFS2]]|0.464|
|[[:human genes:t:trmt10c|TRMT10C]]|0.463|
|[[:human genes:c:coa6|COA6]]|0.463|
|[[:human genes:m:mettl17|METTL17]]|0.462|
|[[:human genes:m:mtif3|MTIF3]]|0.46|
|[[:human genes:c:clpp|CLPP]]|0.458|
|[[:human genes:c:c10orf2|C10orf2]]|0.457|
|[[:human genes:m:mrps7|MRPS7]]|0.457|
|[[:human genes:u:uqcrc2|UQCRC2]]|0.456|
|[[:human genes:p:polg2|POLG2]]|0.456|
|[[:human genes:c:crls1|CRLS1]]|0.456|
|[[:human genes:a:atp5sl|ATP5SL]]|0.454|
|[[:human genes:m:mrpl36|MRPL36]]|0.454|
|[[:human genes:m:mrpl33|MRPL33]]|0.451|
|[[:human genes:n:ndufs5|NDUFS5]]|0.451|
|[[:human genes:t:triap1|TRIAP1]]|0.451|
|[[:human genes:p:pdss1|PDSS1]]|0.449|
|[[:human genes:n:ndufs7|NDUFS7]]|0.445|
|[[:human genes:d:dhx30|DHX30]]|0.445|
|[[:human genes:t:timmdc1|TIMMDC1]]|0.443|
|[[:human genes:m:mtg1|MTG1]]|0.442|
|[[:human genes:a:atp5o|ATP5O]]|0.441|
|[[:human genes:n:ndufaf3|NDUFAF3]]|0.44|
|[[:human genes:g:grsf1|GRSF1]]|0.44|
|[[:human genes:l:lrpprc|LRPPRC]]|0.439|
|[[:human genes:o:opa1|OPA1]]|0.438|
|[[:human genes:m:mrps17|MRPS17]]|0.437|
|[[:human genes:s:slc25a3|SLC25A3]]|0.437|
|[[:human genes:n:ndufb5|NDUFB5]]|0.436|
|[[:human genes:p:polrmt|POLRMT]]|0.436|
|[[:human genes:c:coq3|COQ3]]|0.436|
|[[:human genes:p:pet117|PET117]]|0.435|
|[[:human genes:c:coa5|COA5]]|0.435|
|[[:human genes:a:aars2|AARS2]]|0.435|
|[[:human genes:n:ndufa2|NDUFA2]]|0.434|
|[[:human genes:m:mrpl17|MRPL17]]|0.433|
|[[:human genes:u:uqcc2|UQCC2]]|0.433|
|[[:human genes:a:atp5j|ATP5J]]|0.432|
|[[:human genes:m:mrpl13|MRPL13]]|0.43|
|[[:human genes:r:rtn4ip1|RTN4IP1]]|0.43|
|[[:human genes:g:gtpbp6|GTPBP6]]|0.429|
|[[:human genes:m:mrpl4|MRPL4]]|0.429|
|[[:human genes:m:mrpl55|MRPL55]]|0.428|
|[[:human genes:m:mrpl51|MRPL51]]|0.427|
|[[:human genes:t:timm17a|TIMM17A]]|0.425|
|[[:human genes:t:tfb1m|TFB1M]]|0.425|
|[[:human genes:t:timm8b|TIMM8B]]|0.425|
|[[:human genes:a:atp5l|ATP5L]]|0.425|
|[[:human genes:t:tomm7|TOMM7]]|0.423|
|[[:human genes:m:mrpl9|MRPL9]]|0.423|
|[[:human genes:m:mrpl22|MRPL22]]|0.42|
|[[:human genes:c:c17orf89|C17orf89]]|0.418|
|[[:human genes:m:mterfd1|MTERFD1]]|0.417|
|[[:human genes:u:uqcr10|UQCR10]]|0.414|
|[[:human genes:n:ndufa11|NDUFA11]]|0.413|
|[[:human genes:h:higd2a|HIGD2A]]|0.413|
|[[:human genes:m:mtrf1|MTRF1]]|0.412|
|[[:human genes:c:coa3|COA3]]|0.412|
|[[:human genes:n:ndufb9|NDUFB9]]|0.412|
|[[:human genes:m:mrps22|MRPS22]]|0.41|
|[[:human genes:a:acad9|ACAD9]]|0.409|
|[[:human genes:n:ndufa5|NDUFA5]]|0.407|
|[[:human genes:r:romo1|ROMO1]]|0.407|
|[[:human genes:m:mrpl49|MRPL49]]|0.407|
|[[:human genes:c:cox6c|COX6C]]|0.406|
|[[:human genes:n:nfu1|NFU1]]|0.406|
|[[:human genes:n:ndufaf4|NDUFAF4]]|0.403|
|[[:human genes:m:mrpl18|MRPL18]]|0.403|
|[[:human genes:t:tomm20|TOMM20]]|0.403|
|[[:human genes:a:aifm1|AIFM1]]|0.401|
|[[:human genes:m:mrps27|MRPS27]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 207/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|6/28|
|blood|7/28|
|bone|5/26|
|breast|15/33|
|central nervous system|13/56|
|cervix|3/4|
|colorectal|6/17|
|esophagus|7/13|
|fibroblast|0/1|
|gastric|3/16|
|kidney|4/21|
|liver|5/20|
|lung|18/75|
|lymphocyte|3/16|
|ovary|5/26|
|pancreas|7/24|
|peripheral nervous system|4/16|
|plasma cell|2/15|
|prostate|0/1|
|skin|4/24|
|soft tissue|2/9|
|thyroid|0/2|
|upper aerodigestive|15/22|
|urinary tract|11/29|
|uterus|2/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 307
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.7 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PNPT1 Expression in NALM6 Cells: 7.7'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>