======= POLR2I =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLR2I
  * **<color #00a2e8>Official Name</color>**: RNA polymerase II subunit I
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5438|5438]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P36954|P36954]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLR2I&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLR2I|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/180662|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity). {ECO:0000250, ECO:0000269|PubMed:9852112}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RNA POL M 15KD|
|TFIIS C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter|
|maintenance of transcriptional fidelity during DNA-templated transcription elongation|
|RNA polymerase II, core complex|
|mRNA cleavage|
|DNA-directed 5-3 RNA polymerase activity|
|7-methylguanosine mRNA capping|
|7-methylguanosine RNA capping|
|RNA capping|
|positive regulation of viral transcription|
|regulation of viral transcription|
|somatic stem cell population maintenance|
|snRNA transcription by RNA polymerase II|
|snRNA transcription|
|transcription-coupled nucleotide-excision repair|
|transcription elongation from RNA polymerase II promoter|
|fibroblast growth factor receptor signaling pathway|
|regulation of gene silencing by miRNA|
|regulation of posttranscriptional gene silencing|
|regulation of gene silencing by RNA|
|DNA-templated transcription, elongation|
|ncRNA transcription|
|positive regulation of viral process|
|cellular response to fibroblast growth factor stimulus|
|nucleotide-excision repair|
|response to fibroblast growth factor|
|regulation of gene silencing|
|stem cell population maintenance|
|maintenance of cell number|
|RNA phosphodiester bond hydrolysis|
|transcription initiation from RNA polymerase II promoter|
|nucleic acid binding|
|regulation of viral process|
|DNA-templated transcription, initiation|
|regulation of symbiosis, encompassing mutualism through parasitism|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|mRNA splicing, via spliceosome|
|nucleic acid phosphodiester bond hydrolysis|
|RNA splicing, via transesterification reactions|
|RNA splicing|
|mRNA processing|
|transcription by RNA polymerase II|
|cellular response to growth factor stimulus|
|DNA repair|
|positive regulation of multi-organism process|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|response to growth factor|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|mRNA metabolic process|
|enzyme linked receptor protein signaling pathway|
|DNA metabolic process|
|regulation of multi-organism process|
|cellular response to DNA damage stimulus|
|zinc ion binding|
|nucleolus|
|RNA processing|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|cellular response to endogenous stimulus|
|organic cyclic compound biosynthetic process|
|response to endogenous stimulus|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp34|Rotenone 20μM R00 exp34]]|-2.69|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|-2.46|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|-2.23|
|[[:results:exp17|DABN 20μM R00 exp17]]|-2.14|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|-2.07|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-1.86|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|-1.75|
|[[:results:exp486|Heregulin-B 44ng/ml R08 exp486]]|1.74|
|[[:results:exp471|Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471]]|1.78|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|1.88|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|1.88|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:g:golga6l1|GOLGA6L1]]|0.551|
|[[:human genes:b:brd2|BRD2]]|0.465|
|[[:human genes:p:psmb4|PSMB4]]|0.452|
|[[:human genes:r:rpl35|RPL35]]|0.424|
|[[:human genes:n:ngdn|NGDN]]|0.421|
|[[:human genes:g:golga8o|GOLGA8O]]|0.42|
|[[:human genes:p:psmd2|PSMD2]]|0.411|
|[[:human genes:u:utp3|UTP3]]|0.41|
|[[:human genes:c:cox7a2l|COX7A2L]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 726/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/25|
|breast|33/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|14/14|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1437
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.31 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLR2I Expression in NALM6 Cells: 4.31'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>