======= POLR3B =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLR3B
  * **<color #00a2e8>Official Name</color>**: RNA polymerase III subunit B
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55703|55703]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9NW08|Q9NW08]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLR3B&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLR3B|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/614366|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. {ECO:0000250, ECO:0000269|PubMed:19609254, ECO:0000269|PubMed:19631370}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RNA pol Rpb2 6|
|RNA pol Rpb2 4|
|RNA pol Rpb2 7|
|RNA pol Rpb2 3|
|RNA pol Rpb2 2|
|RNA pol Rpb2 5|
|RNA pol Rpb2 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|ribonucleoside binding|
|RNA polymerase III activity|
|RNA polymerase III complex|
|positive regulation of interferon-beta production|
|DNA-directed 5-3 RNA polymerase activity|
|transcription by RNA polymerase III|
|regulation of interferon-beta production|
|positive regulation of type I interferon production|
|regulation of type I interferon production|
|defense response to virus|
|response to virus|
|positive regulation of innate immune response|
|positive regulation of response to biotic stimulus|
|positive regulation of cytokine production|
|regulation of innate immune response|
|positive regulation of defense response|
|positive regulation of multi-organism process|
|regulation of response to biotic stimulus|
|positive regulation of response to external stimulus|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|regulation of cytokine production|
|regulation of defense response|
|innate immune response|
|regulation of multi-organism process|
|positive regulation of immune response|
|defense response to other organism|
|immune effector process|
|regulation of response to external stimulus|
|nucleobase-containing compound biosynthetic process|
|positive regulation of immune system process|
|regulation of immune response|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|response to other organism|
|organic cyclic compound biosynthetic process|
|response to external biotic stimulus|
|response to biotic stimulus|
|defense response|
|DNA binding|
|regulation of response to stress|
|cellular nitrogen compound biosynthetic process|
|regulation of immune system process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|immune response|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp486|Heregulin-B 44ng/ml R08 exp486]]|-2.34|
|[[:results:exp516|Pyrazinamide 100μM R08 exp516]]|-1.94|
|[[:results:exp100|NFN1 1μM R03 exp100]]|1.71|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|1.78|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|1.78|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|2.03|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|2.03|
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|2.08|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|2.1|
|[[:results:exp152|SGC2043 10μM R03 exp152]]|2.14|
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|2.17|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|2.45|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|2.8|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|3.56|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:polr3h|POLR3H]]|0.637|
|[[:human genes:m:meaf6|MEAF6]]|0.564|
|[[:human genes:r:rptor|RPTOR]]|0.553|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.553|
|[[:human genes:r:rnmt|RNMT]]|0.552|
|[[:human genes:t:trmt5|TRMT5]]|0.539|
|[[:human genes:r:rtcb|RTCB]]|0.529|
|[[:human genes:t:tti2|TTI2]]|0.512|
|[[:human genes:g:gnb1l|GNB1L]]|0.508|
|[[:human genes:e:eif4e|EIF4E]]|0.507|
|[[:human genes:h:hars|HARS]]|0.494|
|[[:human genes:d:dpagt1|DPAGT1]]|0.486|
|[[:human genes:o:otud5|OTUD5]]|0.485|
|[[:human genes:c:crcp|CRCP]]|0.484|
|[[:human genes:t:thg1l|THG1L]]|0.479|
|[[:human genes:a:alg2|ALG2]]|0.478|
|[[:human genes:g:gtf3c2|GTF3C2]]|0.477|
|[[:human genes:s:srp68|SRP68]]|0.475|
|[[:human genes:m:mars|MARS]]|0.474|
|[[:human genes:t:tti1|TTI1]]|0.474|
|[[:human genes:g:gmppb|GMPPB]]|0.469|
|[[:human genes:m:mtor|MTOR]]|0.469|
|[[:human genes:t:trnt1|TRNT1]]|0.467|
|[[:human genes:y:yars|YARS]]|0.466|
|[[:human genes:n:nars|NARS]]|0.466|
|[[:human genes:s:srp72|SRP72]]|0.459|
|[[:human genes:d:dhps|DHPS]]|0.454|
|[[:human genes:c:csnk2b|CSNK2B]]|0.453|
|[[:human genes:v:vmp1|VMP1]]|0.453|
|[[:human genes:p:pmm2|PMM2]]|0.452|
|[[:human genes:d:dad1|DAD1]]|0.45|
|[[:human genes:a:aars|AARS]]|0.444|
|[[:human genes:z:znf259|ZNF259]]|0.444|
|[[:human genes:r:rbm14|RBM14]]|0.444|
|[[:human genes:s:spcs2|SPCS2]]|0.441|
|[[:human genes:c:ccdc101|CCDC101]]|0.44|
|[[:human genes:u:uap1|UAP1]]|0.435|
|[[:human genes:t:tsen34|TSEN34]]|0.435|
|[[:human genes:p:pam16|PAM16]]|0.433|
|[[:human genes:v:vrk1|VRK1]]|0.432|
|[[:human genes:t:tsen2|TSEN2]]|0.432|
|[[:human genes:c:cct7|CCT7]]|0.431|
|[[:human genes:n:nae1|NAE1]]|0.428|
|[[:human genes:g:gfpt1|GFPT1]]|0.427|
|[[:human genes:a:alg1|ALG1]]|0.427|
|[[:human genes:g:gtf3c4|GTF3C4]]|0.426|
|[[:human genes:s:spcs3|SPCS3]]|0.426|
|[[:human genes:m:med16|MED16]]|0.425|
|[[:human genes:p:polr3a|POLR3A]]|0.425|
|[[:human genes:t:tbca|TBCA]]|0.424|
|[[:human genes:p:pelo|PELO]]|0.419|
|[[:human genes:s:ssbp2|SSBP2]]|0.417|
|[[:human genes:t:tbp|TBP]]|0.412|
|[[:human genes:e:eef1e1|EEF1E1]]|0.411|
|[[:human genes:r:rpn1|RPN1]]|0.41|
|[[:human genes:h:hspd1|HSPD1]]|0.408|
|[[:human genes:e:exoc4|EXOC4]]|0.405|
|[[:human genes:m:mllt1|MLLT1]]|0.405|
|[[:human genes:s:spty2d1|SPTY2D1]]|0.404|
|[[:human genes:e:eprs|EPRS]]|0.404|
|[[:human genes:s:slc35b1|SLC35B1]]|0.403|
|[[:human genes:a:alg13|ALG13]]|0.403|
|[[:human genes:t:triap1|TRIAP1]]|0.401|
|[[:human genes:c:cars|CARS]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 719/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/25|
|breast|31/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|21/21|
|liver|20/20|
|lung|74/75|
|lymphocyte|14/14|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|15/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1192
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.06 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLR3B Expression in NALM6 Cells: 5.06'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>