======= PRKAG1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PRKAG1
  * **<color #00a2e8>Official Name</color>**: protein kinase AMP-activated non-catalytic subunit gamma 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5571|5571]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P54619|P54619]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PRKAG1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PRKAG1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602742|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. {ECO:0000269|PubMed:21680840}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|CBS|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|cAMP-dependent protein kinase regulator activity|
|AMP-activated protein kinase activity|
|cAMP-dependent protein kinase activity|
|AMP binding|
|nucleotide-activated protein kinase complex|
|ADP binding|
|regulation of glycolytic process|
|regulation of carbohydrate catabolic process|
|import into nucleus|
|regulation of purine nucleotide metabolic process|
|fatty acid biosynthetic process|
|regulation of nucleotide metabolic process|
|regulation of ATP metabolic process|
|cell cycle arrest|
|regulation of generation of precursor metabolites and energy|
|macroautophagy|
|regulation of macroautophagy|
|regulation of signal transduction by p53 class mediator|
|monocarboxylic acid biosynthetic process|
|regulation of carbohydrate metabolic process|
|protein kinase activity|
|process utilizing autophagic mechanism|
|autophagy|
|nucleocytoplasmic transport|
|nuclear transport|
|carboxylic acid biosynthetic process|
|organic acid biosynthetic process|
|fatty acid metabolic process|
|regulation of autophagy|
|regulation of small molecule metabolic process|
|protein kinase binding|
|regulation of protein serine/threonine kinase activity|
|monocarboxylic acid metabolic process|
|positive regulation of protein kinase activity|
|spermatogenesis|
|male gamete generation|
|negative regulation of cell cycle|
|positive regulation of kinase activity|
|lipid biosynthetic process|
|small molecule biosynthetic process|
|positive regulation of transferase activity|
|gamete generation|
|regulation of protein kinase activity|
|multicellular organismal reproductive process|
|sexual reproduction|
|regulation of cellular catabolic process|
|multicellular organism reproduction|
|regulation of kinase activity|
|carboxylic acid metabolic process|
|cellular lipid metabolic process|
|protein phosphorylation|
|regulation of transferase activity|
|multi-organism reproductive process|
|regulation of catabolic process|
|cell cycle process|
|oxoacid metabolic process|
|positive regulation of protein phosphorylation|
|organic acid metabolic process|
|positive regulation of phosphorylation|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|regulation of cell cycle|
|lipid metabolic process|
|positive regulation of protein modification process|
|phosphorylation|
|cell cycle|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|ATP binding|
|intracellular transport|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|positive regulation of protein metabolic process|
|small molecule metabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|cellular catabolic process|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|regulation of protein modification process|
|positive regulation of gene expression|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|-4.12|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|-3.72|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-3.6|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-3.02|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-2.99|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|-2.95|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|-2.76|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|-2.59|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-2.54|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-2.32|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|-2.3|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|-2.24|
|[[:results:exp275|Citral 75μM R06 exp275]]|-2.23|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|-2.23|
|[[:results:exp333|All-trans-Retinoic-Acid 8μM R07 exp333]]|-2.21|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.16|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|-2.11|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-2.09|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-2|
|[[:results:exp45|Docetaxel 0.002μM R01 exp45]]|-2|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|-1.83|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.76|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|-1.73|
|[[:results:exp249|Vinorelbine 0.001μM R05 exp249]]|-1.72|
|[[:results:exp142|OICR-9429 10μM R03 exp142]]|-1.71|
|[[:results:exp494|Isoniazid 100μM R08 exp494]]|-1.7|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|1.7|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|1.72|
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|2.26|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|2.57|
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|2.59|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|2.66|
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|2.74|
|[[:results:exp452|Azithromycin 100μM R08 exp452]]|2.74|
|[[:results:exp389|PF-06409577 20μM R07 exp389]]|5.88|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|6.01|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:prkaa1|PRKAA1]]|0.709|
|[[:human genes:p:prkab1|PRKAB1]]|0.601|
|[[:human genes:s:strada|STRADA]]|0.569|
|[[:human genes:s:stk11|STK11]]|0.452|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 8923
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.62 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PRKAG1 Expression in NALM6 Cells: 4.62'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>