======= PUM1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PUM1
  * **<color #00a2e8>Official Name</color>**: pumilio RNA binding family member 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9698|9698]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q14671|Q14671]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PUM1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PUM1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607204|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA- binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down- regulation at the onset of differentiation (By similarity). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233). {ECO:0000250|UniProtKB:Q80U78, ECO:0000269|PubMed:18328718, ECO:0000269|PubMed:18776931, ECO:0000269|PubMed:20818387, ECO:0000269|PubMed:20860814, ECO:0000269|PubMed:21397187, ECO:0000269|PubMed:21572425, ECO:0000269|PubMed:21653694, ECO:0000269|PubMed:22345517, ECO:0000269|PubMed:22955276, ECO:0000269|PubMed:25340845, ECO:0000269|PubMed:25768905, ECO:0000269|PubMed:26724866, ECO:0000269|PubMed:28431233}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PUF|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of RIG-I signaling pathway|
|regulation of RIG-I signaling pathway|
|regulation of viral-induced cytoplasmic pattern recognition receptor signaling pathway|
|positive regulation of gene silencing by miRNA|
|positive regulation of posttranscriptional gene silencing|
|production of miRNAs involved in gene silencing by miRNA|
|miRNA binding|
|mRNA destabilization|
|RNA destabilization|
|production of small RNA involved in gene silencing by RNA|
|dsRNA processing|
|gene silencing by miRNA|
|positive regulation of mRNA catabolic process|
|posttranscriptional gene silencing by RNA|
|posttranscriptional gene silencing|
|cytoplasmic stress granule|
|regulation of defense response to virus|
|mRNA 3-UTR binding|
|adult locomotory behavior|
|positive regulation of mRNA metabolic process|
|P-body|
|regulation of gene silencing by miRNA|
|gene silencing by RNA|
|regulation of gene silencing by RNA|
|regulation of posttranscriptional gene silencing|
|regulation of chromosome segregation|
|regulation of gene silencing|
|negative regulation of translation|
|adult behavior|
|negative regulation of cellular amide metabolic process|
|gene silencing|
|stem cell differentiation|
|regulation of mRNA stability|
|regulation of RNA stability|
|locomotory behavior|
|regulation of mRNA catabolic process|
|positive regulation of immune effector process|
|regulation of gene expression, epigenetic|
|regulation of mRNA metabolic process|
|positive regulation of innate immune response|
|regulation of translation|
|positive regulation of response to biotic stimulus|
|positive regulation of cellular catabolic process|
|nuclear speck|
|regulation of cellular amide metabolic process|
|positive regulation of catabolic process|
|positive regulation of cytokine production|
|regulation of innate immune response|
|regulation of immune effector process|
|positive regulation of defense response|
|positive regulation of multi-organism process|
|regulation of response to biotic stimulus|
|posttranscriptional regulation of gene expression|
|spermatogenesis|
|male gamete generation|
|behavior|
|positive regulation of response to external stimulus|
|gamete generation|
|regulation of cytokine production|
|regulation of defense response|
|regulation of multi-organism process|
|multicellular organismal reproductive process|
|sexual reproduction|
|regulation of cellular catabolic process|
|multicellular organism reproduction|
|positive regulation of immune response|
|RNA processing|
|regulation of catabolic process|
|multi-organism reproductive process|
|positive regulation of intracellular signal transduction|
|negative regulation of cellular protein metabolic process|
|regulation of response to external stimulus|
|negative regulation of protein metabolic process|
|regulation of immune response|
|positive regulation of immune system process|
|regulation of cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|reproductive process|
|reproduction|
|negative regulation of macromolecule biosynthetic process|
|regulation of response to stress|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|positive regulation of signal transduction|
|regulation of immune system process|
|RNA metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|positive regulation of nucleobase-containing compound metabolic process|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|-1.9|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|-1.8|
|[[:results:exp142|OICR-9429 10μM R03 exp142]]|-1.72|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-1.71|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|1.73|
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.82|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|1.86|
|[[:results:exp405|Tenofovir 10μM R07 exp405]]|1.9|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|2.05|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|2.07|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|2.2|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|2.28|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|2.29|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|2.38|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|2.59|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hars|HARS]]|0.452|
|[[:human genes:m:meaf6|MEAF6]]|0.429|
|[[:human genes:t:thoc6|THOC6]]|0.41|
|[[:human genes:t:trim28|TRIM28]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 2/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 10464
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.01 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PUM1 Expression in NALM6 Cells: 7.01'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>