======= RPA1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPA1
  * **<color #00a2e8>Official Name</color>**: replication protein A1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6117|6117]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P27694|P27694]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPA1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPA1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/179835|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:27723720, PubMed:27723717). Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single- stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S- phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {ECO:0000269|PubMed:12791985, ECO:0000269|PubMed:17765923, ECO:0000269|PubMed:17959650, ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:19996105, ECO:0000269|PubMed:24332808, ECO:0000269|PubMed:27723717, ECO:0000269|PubMed:27723720, ECO:0000269|PubMed:7697716, ECO:0000269|PubMed:7700386, ECO:0000269|PubMed:9430682, ECO:0000269|PubMed:9765279}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|tRNA anti|
|Rep fac-A C|
|Rep-A N|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|single-stranded telomeric DNA binding|
|G-rich strand telomeric DNA binding|
|DNA unwinding involved in DNA replication|
|DNA replication factor A complex|
|site of DNA damage|
|error-free translesion synthesis|
|error-prone translesion synthesis|
|nucleotide-excision repair, preincision complex stabilization|
|nucleotide-excision repair, DNA incision, 3-to lesion|
|telomere maintenance via telomerase|
|nucleotide-excision repair, DNA gap filling|
|telomere maintenance via semi-conservative replication|
|nucleotide-excision repair, preincision complex assembly|
|telomere maintenance via telomere lengthening|
|mismatch repair|
|RNA-dependent DNA biosynthetic process|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|base-excision repair|
|DNA damage response, detection of DNA damage|
|nucleotide-excision repair, DNA incision|
|translesion synthesis|
|nuclear DNA replication|
|cell cycle DNA replication|
|DNA synthesis involved in DNA repair|
|interstrand cross-link repair|
|postreplication repair|
|damaged DNA binding|
|protein localization to chromosome|
|transcription-coupled nucleotide-excision repair|
|regulation of cellular response to heat|
|double-strand break repair via homologous recombination|
|recombinational repair|
|telomere maintenance|
|PML body|
|telomere organization|
|single-stranded DNA binding|
|DNA biosynthetic process|
|nuclear chromosome, telomeric region|
|DNA duplex unwinding|
|nucleotide-excision repair|
|G1/S transition of mitotic cell cycle|
|DNA geometric change|
|cell cycle G1/S phase transition|
|DNA-dependent DNA replication|
|regulation of signal transduction by p53 class mediator|
|double-strand break repair|
|protein-DNA complex assembly|
|DNA replication|
|DNA recombination|
|meiotic cell cycle|
|protein-DNA complex subunit organization|
|positive regulation of protein complex assembly|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|DNA conformation change|
|nucleic acid phosphodiester bond hydrolysis|
|anatomical structure homeostasis|
|sequence-specific DNA binding|
|regulation of protein complex assembly|
|DNA repair|
|positive regulation of cellular component biogenesis|
|mitotic cell cycle process|
|mitotic cell cycle|
|detection of stimulus|
|regulation of cellular response to stress|
|protein localization to organelle|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cellular protein-containing complex assembly|
|cellular homeostasis|
|regulation of cellular component biogenesis|
|cell cycle process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|positive regulation of cellular component organization|
|organic cyclic compound biosynthetic process|
|cell cycle|
|reproductive process|
|reproduction|
|regulation of response to stress|
|protein-containing complex assembly|
|cellular protein localization|
|cellular macromolecule localization|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of intracellular signal transduction|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|-1.85|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-1.79|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|-1.7|
|[[:results:exp494|Isoniazid 100μM R08 exp494]]|1.73|
|[[:results:exp493|IL-3 9ng/ml R08 exp493]]|1.74|
|[[:results:exp247|UM0130462 0.025 to 0.035μM day4 R05 exp247]]|1.81|
|[[:results:exp149|SB203580 25μM R03 exp149]]|1.83|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|1.87|
|[[:results:exp263|Aphidicolin 0.04μM R06 exp263]]|1.88|
|[[:results:exp274|Citral 50μM R06 exp274]]|1.89|
|[[:results:exp295|Pyronaridine 1μM R06 exp295]]|1.93|
|[[:results:exp273|Cisplatin 0.35μM R06 exp273]]|1.94|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|1.96|
|[[:results:exp249|Vinorelbine 0.001μM R05 exp249]]|2.05|
|[[:results:exp139|Nicotinamide Riboside 100μM R03 exp139]]|2.08|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|2.13|
|[[:results:exp303|39°C R06 exp303]]|2.21|
|[[:results:exp279|D-Fructose 10000μM R06 exp279]]|2.25|
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|2.25|
|[[:results:exp476|Dihydrosphingosine 8μM R08 exp476]]|3.17|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.627|
|[[:human genes:p:prim1|PRIM1]]|0.503|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 735/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|27/28|
|blood|28/28|
|bone|26/26|
|breast|33/33|
|central nervous system|55/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|20/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|14/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 186
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.68 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPA1 Expression in NALM6 Cells: 7.68'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>