======= TRIM28 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: TRIM28
  * **<color #00a2e8>Official Name</color>**: tripartite motif containing 28
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10155|10155]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q13263|Q13263]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=TRIM28&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20TRIM28|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601742|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). Acts as a corepressor for ZFP568 (By similarity). {ECO:0000250|UniProtKB:Q62318, ECO:0000269|PubMed:10347202, ECO:0000269|PubMed:11959841, ECO:0000269|PubMed:15882967, ECO:0000269|PubMed:16107876, ECO:0000269|PubMed:16862143, ECO:0000269|PubMed:17079232, ECO:0000269|PubMed:17178852, ECO:0000269|PubMed:17704056, ECO:0000269|PubMed:17942393, ECO:0000269|PubMed:18060868, ECO:0000269|PubMed:18082607, ECO:0000269|PubMed:20424263, ECO:0000269|PubMed:20858735, ECO:0000269|PubMed:20864041, ECO:0000269|PubMed:21940674, ECO:0000269|PubMed:23543754, ECO:0000269|PubMed:23665872, ECO:0000269|PubMed:24623306, ECO:0000269|PubMed:27029610, ECO:0000269|PubMed:8769649, ECO:0000269|PubMed:9016654}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PHD|
|zf-B box|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|Krueppel-associated box domain binding|
|negative regulation of DNA demethylation|
|regulation of genetic imprinting|
|chromo shadow domain binding|
|convergent extension involved in axis elongation|
|regulation of DNA demethylation|
|changes to DNA methylation involved in embryo development|
|DNA methylation involved in embryo development|
|positive regulation of methylation-dependent chromatin silencing|
|regulation of methylation-dependent chromatin silencing|
|positive regulation of chromatin silencing|
|convergent extension|
|negative regulation of single stranded viral RNA replication via double stranded DNA intermediate|
|negative regulation of viral release from host cell|
|SUMO transferase activity|
|regulation of single stranded viral RNA replication via double stranded DNA intermediate|
|nuclear heterochromatin|
|embryonic placenta morphogenesis|
|axis elongation|
|nuclear euchromatin|
|regulation of viral release from host cell|
|embryo implantation|
|positive regulation of protein import into nucleus|
|regulation of chromatin silencing|
|positive regulation of protein import|
|DNA methylation|
|DNA alkylation|
|promoter-specific chromatin binding|
|regulation of protein import into nucleus|
|positive regulation of DNA binding|
|negative regulation of viral genome replication|
|regulation of protein import|
|positive regulation of nucleocytoplasmic transport|
|DNA methylation or demethylation|
|positive regulation of DNA repair|
|protein sumoylation|
|epithelial to mesenchymal transition|
|RNA polymerase II transcription factor complex|
|positive regulation of protein localization to nucleus|
|negative regulation of viral life cycle|
|embryonic placenta development|
|DNA modification|
|regulation of viral genome replication|
|negative regulation of viral process|
|positive regulation of response to DNA damage stimulus|
|positive regulation of chromatin organization|
|regulation of nucleocytoplasmic transport|
|developmental growth involved in morphogenesis|
|chromatin|
|regulation of protein localization to nucleus|
|negative regulation of DNA metabolic process|
|regulation of DNA repair|
|regulation of DNA binding|
|regulation of gene silencing|
|regulation of viral life cycle|
|placenta development|
|positive regulation of intracellular protein transport|
|mesenchymal cell differentiation|
|positive regulation of chromosome organization|
|transcription initiation from RNA polymerase II promoter|
|positive regulation of binding|
|female pregnancy|
|regulation of chromatin organization|
|protein autophosphorylation|
|positive regulation of DNA metabolic process|
|regulation of viral process|
|positive regulation of intracellular transport|
|multi-multicellular organism process|
|negative regulation of multi-organism process|
|DNA-templated transcription, initiation|
|regulation of response to DNA damage stimulus|
|regulation of symbiosis, encompassing mutualism through parasitism|
|mesenchyme development|
|regulation of intracellular protein transport|
|protein kinase activity|
|transcription corepressor activity|
|ubiquitin-protein transferase activity|
|Ras protein signal transduction|
|macromolecule methylation|
|transcription coactivator activity|
|ubiquitin protein ligase binding|
|methylation|
|peptidyl-lysine modification|
|small GTPase mediated signal transduction|
|negative regulation of locomotion|
|positive regulation of cellular protein localization|
|regulation of chromosome organization|
|regulation of intracellular transport|
|regulation of DNA metabolic process|
|regulation of binding|
|in utero embryonic development|
|developmental growth|
|growth|
|chromatin binding|
|sequence-specific DNA binding|
|reproductive structure development|
|positive regulation of protein transport|
|reproductive system development|
|morphogenesis of an epithelium|
|embryonic organ development|
|positive regulation of establishment of protein localization|
|transcription by RNA polymerase II|
|DNA repair|
|protein complex oligomerization|
|regulation of cellular protein localization|
|tissue morphogenesis|
|embryonic morphogenesis|
|positive regulation of organelle organization|
|chordate embryonic development|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|embryo development ending in birth or egg hatching|
|RNA biosynthetic process|
|developmental process involved in reproduction|
|protein ubiquitination|
|chromatin organization|
|viral process|
|regulation of protein transport|
|regulation of cellular response to stress|
|regulation of peptide transport|
|DNA metabolic process|
|regulation of establishment of protein localization|
|innate immune response|
|protein modification by small protein conjugation|
|cellular response to DNA damage stimulus|
|regulation of multi-organism process|
|symbiotic process|
|interspecies interaction between organisms|
|zinc ion binding|
|negative regulation of transcription by RNA polymerase II|
|peptidyl-amino acid modification|
|regulation of cellular localization|
|defense response to other organism|
|protein phosphorylation|
|embryo development|
|regulation of locomotion|
|protein modification by small protein conjugation or removal|
|positive regulation of transport|
|multi-organism reproductive process|
|regulation of protein localization|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|epithelium development|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of transcription, DNA-templated|
|positive regulation of cellular component organization|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|phosphorylation|
|regulation of organelle organization|
|response to other organism|
|organic cyclic compound biosynthetic process|
|response to external biotic stimulus|
|response to biotic stimulus|
|negative regulation of RNA metabolic process|
|defense response|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|reproductive process|
|reproduction|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|regulation of response to stress|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|protein-containing complex assembly|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|RNA metabolic process|
|intracellular signal transduction|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|tissue development|
|macromolecule biosynthetic process|
|positive regulation of molecular function|
|protein-containing complex subunit organization|
|regulation of transport|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp75|MK-1775 0.32μM R02 exp75]]|-2.05|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|-1.95|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|-1.71|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|1.79|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|1.8|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|1.82|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|1.86|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|2.06|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|2.08|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|2.1|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|2.13|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|2.25|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|2.37|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|2.57|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|2.77|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|2.8|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|2.95|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|4.01|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:n:nsd1|NSD1]]|0.642|
|[[:human genes:i:id3|ID3]]|0.602|
|[[:human genes:d:dohh|DOHH]]|0.582|
|[[:human genes:m:mllt1|MLLT1]]|0.573|
|[[:human genes:z:znf592|ZNF592]]|0.563|
|[[:human genes:r:rptor|RPTOR]]|0.561|
|[[:human genes:b:bap1|BAP1]]|0.551|
|[[:human genes:m:mtor|MTOR]]|0.539|
|[[:human genes:m:mlst8|MLST8]]|0.534|
|[[:human genes:m:med23|MED23]]|0.526|
|[[:human genes:a:arl14ep|ARL14EP]]|0.521|
|[[:human genes:b:brpf1|BRPF1]]|0.519|
|[[:human genes:w:wdr24|WDR24]]|0.508|
|[[:human genes:f:faf2|FAF2]]|0.506|
|[[:human genes:i:irf2bp2|IRF2BP2]]|0.504|
|[[:human genes:c:ccdc101|CCDC101]]|0.502|
|[[:human genes:o:otud5|OTUD5]]|0.5|
|[[:human genes:z:znf608|ZNF608]]|0.5|
|[[:human genes:f:fbxo11|FBXO11]]|0.499|
|[[:human genes:h:hars|HARS]]|0.497|
|[[:human genes:h:hhex|HHEX]]|0.491|
|[[:human genes:p:pfn1|PFN1]]|0.484|
|[[:human genes:b:bak1|BAK1]]|0.482|
|[[:human genes:u:ube2g2|UBE2G2]]|0.482|
|[[:human genes:p:plaa|PLAA]]|0.482|
|[[:human genes:s:setdb1|SETDB1]]|0.479|
|[[:human genes:r:rnmt|RNMT]]|0.476|
|[[:human genes:c:cbx3|CBX3]]|0.475|
|[[:human genes:t:thg1l|THG1L]]|0.473|
|[[:human genes:c:cmtr1|CMTR1]]|0.463|
|[[:human genes:t:tle4|TLE4]]|0.461|
|[[:human genes:v:vhl|VHL]]|0.446|
|[[:human genes:d:dr1|DR1]]|0.444|
|[[:human genes:x:xpo6|XPO6]]|0.444|
|[[:human genes:a:atf7ip|ATF7IP]]|0.443|
|[[:human genes:g:gfpt1|GFPT1]]|0.437|
|[[:human genes:t:trip12|TRIP12]]|0.437|
|[[:human genes:m:med25|MED25]]|0.434|
|[[:human genes:z:znf445|ZNF445]]|0.434|
|[[:human genes:e:exoc4|EXOC4]]|0.434|
|[[:human genes:c:casp9|CASP9]]|0.429|
|[[:human genes:p:ppp2r3c|PPP2R3C]]|0.429|
|[[:human genes:n:nae1|NAE1]]|0.429|
|[[:human genes:u:ubqln1|UBQLN1]]|0.426|
|[[:human genes:p:pdpk1|PDPK1]]|0.426|
|[[:human genes:c:cbfb|CBFB]]|0.426|
|[[:human genes:p:pdap1|PDAP1]]|0.424|
|[[:human genes:a:asxl2|ASXL2]]|0.424|
|[[:human genes:p:pmm2|PMM2]]|0.423|
|[[:human genes:s:serf2|SERF2]]|0.422|
|[[:human genes:b:bptf|BPTF]]|0.421|
|[[:human genes:m:meaf6|MEAF6]]|0.421|
|[[:human genes:t:tti2|TTI2]]|0.42|
|[[:human genes:u:uap1|UAP1]]|0.419|
|[[:human genes:r:romo1|ROMO1]]|0.418|
|[[:human genes:g:gmppb|GMPPB]]|0.418|
|[[:human genes:t:triap1|TRIAP1]]|0.416|
|[[:human genes:a:apaf1|APAF1]]|0.416|
|[[:human genes:a:alg13|ALG13]]|0.416|
|[[:human genes:u:usp22|USP22]]|0.415|
|[[:human genes:p:ppp6c|PPP6C]]|0.415|
|[[:human genes:t:taf4|TAF4]]|0.415|
|[[:human genes:a:aars|AARS]]|0.415|
|[[:human genes:s:scaf8|SCAF8]]|0.414|
|[[:human genes:w:wdr59|WDR59]]|0.413|
|[[:human genes:h:hccs|HCCS]]|0.412|
|[[:human genes:d:ddx42|DDX42]]|0.412|
|[[:human genes:a:asb7|ASB7]]|0.412|
|[[:human genes:o:opa1|OPA1]]|0.409|
|[[:human genes:g:gtf3c4|GTF3C4]]|0.407|
|[[:human genes:v:vdac2|VDAC2]]|0.405|
|[[:human genes:m:med16|MED16]]|0.405|
|[[:human genes:p:prkrir|PRKRIR]]|0.401|
|[[:human genes:d:dpagt1|DPAGT1]]|0.401|
|[[:human genes:p:pum1|PUM1]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1852
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.08 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='TRIM28 Expression in NALM6 Cells: 9.08'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>