======= USP8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: USP8
  * **<color #00a2e8>Official Name</color>**: ubiquitin specific peptidase 8
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9101|9101]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P40818|P40818]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=USP8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20USP8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603158|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. {ECO:0000269|PubMed:16520378, ECO:0000269|PubMed:17711858, ECO:0000269|PubMed:18329369, ECO:0000269|PubMed:9628861}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DUF1873|
|Rhodanese|
|UCH|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|regulation of protein catabolic process at postsynapse, modulating synaptic transmission|
|extrinsic component of endosome membrane|
|postsynaptic modulation of chemical synaptic transmission|
|extrinsic component of plasma membrane|
|cellular response to dexamethasone stimulus|
|protein K63-linked deubiquitination|
|protein K48-linked deubiquitination|
|response to dexamethasone|
|cellular response to nerve growth factor stimulus|
|response to nerve growth factor|
|cellular response to glucocorticoid stimulus|
|cellular response to corticosteroid stimulus|
|mitotic cytokinesis|
|endosome organization|
|cysteine-type endopeptidase activity|
|cellular response to ketone|
|response to antineoplastic agent|
|cytoskeleton-dependent cytokinesis|
|cytokinesis|
|thiol-dependent ubiquitin-specific protease activity|
|SH3 domain binding|
|positive regulation of canonical Wnt signaling pathway|
|response to glucocorticoid|
|dendritic spine|
|midbody|
|response to corticosteroid|
|positive regulation of Wnt signaling pathway|
|cellular response to xenobiotic stimulus|
|cellular response to steroid hormone stimulus|
|response to ketone|
|Ras protein signal transduction|
|postsynaptic density|
|early endosome|
|regulation of canonical Wnt signaling pathway|
|protein deubiquitination|
|regulation of protein stability|
|protein modification by small protein removal|
|response to xenobiotic stimulus|
|vesicle organization|
|cadherin binding|
|small GTPase mediated signal transduction|
|response to steroid hormone|
|glutamatergic synapse|
|regulation of Wnt signaling pathway|
|regulation of protein catabolic process|
|cellular response to drug|
|endomembrane system organization|
|modulation of chemical synaptic transmission|
|regulation of trans-synaptic signaling|
|cell division|
|cellular response to growth factor stimulus|
|cellular response to lipid|
|ubiquitin-dependent protein catabolic process|
|response to growth factor|
|modification-dependent protein catabolic process|
|cellular response to organic cyclic compound|
|modification-dependent macromolecule catabolic process|
|proteolysis involved in cellular protein catabolic process|
|mitotic cell cycle process|
|cellular response to hormone stimulus|
|cellular protein catabolic process|
|protein catabolic process|
|mitotic cell cycle|
|response to lipid|
|cellular macromolecule catabolic process|
|response to hormone|
|response to organic cyclic compound|
|protein modification by small protein conjugation or removal|
|regulation of catabolic process|
|cell cycle process|
|response to drug|
|regulation of protein localization|
|macromolecule catabolic process|
|cellular response to oxygen-containing compound|
|organonitrogen compound catabolic process|
|cellular response to endogenous stimulus|
|proteolysis|
|cell cycle|
|response to endogenous stimulus|
|response to oxygen-containing compound|
|positive regulation of signal transduction|
|intracellular signal transduction|
|organic substance catabolic process|
|cellular catabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp539|42°C R08 exp539]]|-2.51|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|-2.29|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|-2.19|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-2.17|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2.16|
|[[:results:exp289|Hydroxyurea 15μM R06 exp289]]|-2.12|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|-2.01|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|-1.95|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|-1.89|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|-1.88|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|-1.81|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-1.79|
|[[:results:exp17|DABN 20μM R00 exp17]]|-1.78|
|[[:results:exp236|GSK2606414 1μM R05 exp236]]|-1.74|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|-1.73|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|-1.71|
|[[:results:exp153|SGC2096 2.6μM R03 exp153]]|1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:polr2j3|POLR2J3]]|0.422|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 286/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|7/28|
|blood|16/28|
|bone|6/26|
|breast|17/33|
|central nervous system|14/56|
|cervix|0/4|
|colorectal|7/17|
|esophagus|4/13|
|fibroblast|0/1|
|gastric|7/16|
|kidney|7/21|
|liver|8/20|
|lung|34/75|
|lymphocyte|8/16|
|ovary|13/26|
|pancreas|7/24|
|peripheral nervous system|2/16|
|plasma cell|10/15|
|prostate|1/1|
|skin|12/24|
|soft tissue|4/9|
|thyroid|1/2|
|upper aerodigestive|9/22|
|urinary tract|14/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1958
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.08 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='USP8 Expression in NALM6 Cells: 7.08'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>