======= XRCC4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: XRCC4
  * **<color #00a2e8>Official Name</color>**: X-ray repair cross complementing 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7518|7518]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q13426|Q13426]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=XRCC4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20XRCC4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/194363|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. {ECO:0000269|PubMed:10757784, ECO:0000269|PubMed:10854421, ECO:0000269|PubMed:16412978, ECO:0000269|PubMed:8548796}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|XRCC4|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|DNA ligase IV complex|
|DNA-dependent protein kinase-DNA ligase 4 complex|
|immunoglobulin V(D)J recombination|
|positive regulation of ligase activity|
|nonhomologous end joining complex|
|establishment of integrated proviral latency|
|DNA ligation involved in DNA repair|
|regulation of ligase activity|
|establishment of viral latency|
|viral latency|
|ligase activity|
|cellular response to lithium ion|
|DNA ligation|
|V(D)J recombination|
|response to lithium ion|
|somatic recombination of immunoglobulin gene segments|
|condensed chromosome|
|response to X-ray|
|somatic diversification of immunoglobulins|
|somatic cell DNA recombination|
|somatic diversification of immune receptors via germline recombination within a single locus|
|somatic diversification of immune receptors|
|double-strand break repair via nonhomologous end joining|
|non-recombinational repair|
|response to ionizing radiation|
|immunoglobulin production|
|production of molecular mediator of immune response|
|double-strand break repair|
|protein C-terminus binding|
|cellular response to metal ion|
|cellular response to inorganic substance|
|DNA recombination|
|response to metal ion|
|response to radiation|
|DNA repair|
|response to inorganic substance|
|immune system development|
|viral process|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|symbiotic process|
|interspecies interaction between organisms|
|identical protein binding|
|response to abiotic stimulus|
|positive regulation of catalytic activity|
|DNA binding|
|cellular response to stress|
|positive regulation of molecular function|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|-2.59|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|-2.09|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.86|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|1.71|
|[[:results:exp287|HMS-I2 5μM R06 exp287]]|1.94|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|1.95|
|[[:results:exp463|Caffeine 2600μM R08 exp463]]|2.03|
|[[:results:exp218|A-395 10μM R05 exp218]]|2.31|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9507
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 3.73 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='XRCC4 Expression in NALM6 Cells: 3.73'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>