====== UMK57 0.6μM R03 exp107 ======
==== Mechanism of Action ====
Potentiates kinesin-13 (MCAK), destabilizes kinetochore-MT attachment, suppresses chromosome mis-segregation
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Mitotic Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%5-(4-Methylphenyl)-4-pyrrolidinylthiopheno[2,3-d]pyrimidine%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 342595-74-8
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/693939|693939]]
    * **<color #00a2e8>IUPAC:</color>** %%5-(4-methylphenyl)-4-pyrrolidin-1-ylthieno[2,3-d]pyrimidine%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C17H17N3S/c1-12-4-6-13(7-5-12)14-10-21-17-15(14)16(18-11-19-17)20-8-2-3-9-20/h4-7,10-11H,2-3,8-9H2,1H3
    * **<color #00a2e8>INCHI Key:</color>** VOAWQTDHSSKEKA-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 295.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC1=CC=C(C=C1)C2=CSC3=NC=NC(=C23)N4CCCC4
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C17H17N3S
{{:chemogenomics:structures:chem-0039.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** In house - University of Montreal - Chemistry Platform
    * **<color #00a2e8>Catalog #:</color>** N/A
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** [M+H]+ Calcd for C17H18N3S 296.1216; found  296.1142
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** UMK57
    * **<color #00a2e8>Min:</color>** -2.8530; **<color #00a2e8>Max:</color>** 75.4945
{{:chemogenomics:dose_response:dr_159.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.3698    |
| IC30  |0.6436    |
| IC40  |1.0136    |
| IC50  |1.5379    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 03
    * **<color #00a2e8>Dose</color>**: 600nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 7.7
    * **<color #00a2e8>Numbers of reads</color>**: 11763207


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|5/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/UMK57_0.6uM_Round-3_exp107.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='UMK57 0.6μM R03 exp107'>
{{:chemogenomics:cranks_plots:exp107.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:T:TACC3|TACC3]]|-4.25|<0.01|
|[[:human_genes:K:KNTC1|KNTC1]]|-3.59|<0.01|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-3.04|<0.01|
|[[:human_genes:G:GTSE1|GTSE1]]|-3.00|<0.01|
|[[:human_genes:G:GAK|GAK]]|-2.79|0.01|
|[[:human_genes:F:FAF2|FAF2]]|-2.74|0.10|
|[[:human_genes:S:SKIV2L|SKIV2L]]|-2.38|0.26|
|[[:human_genes:B:BRD1|BRD1]]|-2.37|0.11|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.36|0.47|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.35|0.28|
|[[:human_genes:M:MSI2|MSI2]]|-2.35|0.11|
|[[:human_genes:P:PRICKLE1|PRICKLE1]]|-2.33|0.11|
|[[:human_genes:C:CWC27|CWC27]]|-2.32|0.11|
|[[:human_genes:C:CNOT2|CNOT2]]|-2.23|0.16|
|[[:human_genes:R:RNF169|RNF169]]|-2.23|0.16|
|[[:human_genes:H:H2AFJ|H2AFJ]]|-2.20|0.17|
|[[:human_genes:S:SNF8|SNF8]]|-2.19|0.28|
|[[:human_genes:G:GPR137C|GPR137C]]|-2.17|0.41|
|[[:human_genes:N:NHLRC2|NHLRC2]]|-2.16|0.41|
|[[:human_genes:D:DLGAP5|DLGAP5]]|-2.15|0.21|
|[[:human_genes:D:DDX55|DDX55]]|-2.10|0.41|
|[[:human_genes:K:KIF18A|KIF18A]]|-2.10|0.35|
|[[:human_genes:U:UBE2N|UBE2N]]|-2.05|0.29|
|[[:human_genes:T:TBX3|TBX3]]|-2.02|0.33|
|[[:human_genes:S:SPDL1|SPDL1]]|-2.01|0.41|
|[[:human_genes:V:VPS25|VPS25]]|-2.01|0.59|
|[[:human_genes:K:KRTAP13-4|KRTAP13-4]]|-2.00|0.41|
|[[:human_genes:S:SYNE3|SYNE3]]|-1.99|0.35|
|[[:human_genes:P:PSMD14|PSMD14]]|-1.99|0.73|
|[[:human_genes:P:PRMT10|PRMT10]]|-1.98|0.35|
|[[:human_genes:C:CETN1|CETN1]]|1.93|0.62|
|[[:human_genes:K:KIF11|KIF11]]|1.94|0.74|
|[[:human_genes:T:THYN1|THYN1]]|1.94|0.62|
|[[:human_genes:C:COPB2|COPB2]]|1.96|0.65|
|[[:human_genes:Y:YWHAE|YWHAE]]|1.98|0.61|
|[[:human_genes:S:SMR3A|SMR3A]]|1.99|0.62|
|[[:human_genes:K:KIF4A|KIF4A]]|1.99|0.61|
|[[:human_genes:E:EEF1A1|EEF1A1]]|2.00|0.62|
|[[:human_genes:U:UTP20|UTP20]]|2.00|0.63|
|[[:human_genes:P:PLN|PLN]]|2.00|0.66|
|[[:human_genes:F:FARSA|FARSA]]|2.01|0.62|
|[[:human_genes:T:TLE4|TLE4]]|2.03|0.62|
|[[:human_genes:P:POLA1|POLA1]]|2.05|0.65|
|[[:human_genes:E:EIF3E|EIF3E]]|2.05|0.62|
|[[:human_genes:S:SRSF3|SRSF3]]|2.07|0.61|
|[[:human_genes:R:RNF40|RNF40]]|2.07|0.61|
|[[:human_genes:H:HNRNPD|HNRNPD]]|2.07|0.54|
|[[:human_genes:P:POP4|POP4]]|2.08|0.61|
|[[:human_genes:S:SSFA2|SSFA2]]|2.12|0.54|
|[[:human_genes:I:IL6ST|IL6ST]]|2.12|0.54|
|[[:human_genes:P:PI4KA|PI4KA]]|2.13|0.61|
|[[:human_genes:W:WASF1|WASF1]]|2.14|0.54|
|[[:human_genes:D:DAZL|DAZL]]|2.14|0.54|
|[[:human_genes:T:TTLL6|TTLL6]]|2.21|0.54|
|[[:human_genes:V:VAMP4|VAMP4]]|2.23|0.54|
|[[:human_genes:M:MRPL33|MRPL33]]|2.23|0.62|
|[[:human_genes:C:CNN1|CNN1]]|2.25|0.54|
|[[:human_genes:C:CASP8AP2|CASP8AP2]]|2.27|0.62|
|[[:human_genes:W:WDR75|WDR75]]|2.36|0.54|
|[[:human_genes:E:EIF5B|EIF5B]]|2.65|0.62|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|0.051|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|0.126|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.085|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|0.075|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.069|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|0.067|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.067|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.067|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.062|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.062|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.062|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|0.059|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|0.058|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|0.056|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|0.055|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp223|Cabazitaxel 0.001μM R05 exp223]]|0.053|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|0.051|<button type="primary" size="small" modal="17">View</button>|
</modal>


<modal id="1" size="lg" title="UMK57 0.6μM R03 exp107  vs  Vincristine 0.0005μM R04 exp190">
{{:chemogenomics:comparison_plots:exp107_vs_exp190.png?nolink |}}
</modal>

<modal id="2" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp310.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Combretastatin A4 0.002 to 0.003μM day4 R04 exp179  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp179.png?nolink |}}
</modal>

<modal id="4" size="lg" title="BI-D1870 2μM R04 exp162  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp162.png?nolink |}}
</modal>

<modal id="5" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp311.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Docosahexaenoic-acid 50μM R07 exp356  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp356.png?nolink |}}
</modal>

<modal id="7" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp312.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Ixabepilone 0.004 to 0.005μM on day4 R04 exp184  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp184.png?nolink |}}
</modal>

<modal id="9" size="lg" title="BML-284 0.09μM R08 exp460  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp460.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Quercetin 20μM R08 exp517  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp517.png?nolink |}}
</modal>

<modal id="11" size="lg" title="S-trityl-L-cysteine 0.5μM R05 exp243  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp243.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Mdivi-1 15μM R05 exp217  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp217.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Epothilone-B 0.0015μM R05 exp231  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp231.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Docetaxel 0.001 to 0.002μM on day4 R04 exp159  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp159.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp228.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Cabazitaxel 0.001μM R05 exp223  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp223.png?nolink |}}
</modal>

<modal id="17" size="lg" title="IU1-C 25μM R04 exp183  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp183.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|ESCRT II complex|592.11|SNF8,VPS25|
|mitotic sister chromatid segregation|21.61|KNTC1,PRICKLE1,DLGAP5,KIF18A,SPDL1|
|sister chromatid segregation|21.00|KNTC1,PRICKLE1,DLGAP5,KIF18A,SPDL1|
|mitotic nuclear division|18.05|KNTC1,PRICKLE1,DLGAP5,KIF18A,SPDL1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>