====== Colchicine 0.009μM R05 exp215 ======
==== Mechanism of Action ====
Inhibits microtubule polymerization
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Microtubule Poison
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Colchicine%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 64-86-8
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/6167|6167]]
    * **<color #00a2e8>IUPAC:</color>** %%N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[a]heptalen-7-yl]acetamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C22H25NO6/c1-12(24)23-16-8-6-13-10-19(27-3)21(28-4)22(29-5)20(13)14-7-9-18(26-2)17(25)11-15(14)16/h7,9-11,16H,6,8H2,1-5H3,(H,23,24)/t16-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** IAKHMKGGTNLKSZ-INIZCTEOSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 399.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC(=O)NC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
    * **<color #00a2e8>Isomeric SMILES:</color>** CC(=O)N[C@H]1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
    * **<color #00a2e8>Molecular Formula:</color>** C22H25NO6
{{:chemogenomics:structures:chem-0111.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** C9754
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C22H25NO6 400.17546; found 400.17564
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Colchicine
    * **<color #00a2e8>Min:</color>** -2.2590; **<color #00a2e8>Max:</color>** 86.4027
{{:chemogenomics:dose_response:dr_198.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0071    |
| IC30  |0.0091    |
| IC40  |0.0111    |
| IC50  |0.0133    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 05
    * **<color #00a2e8>Dose</color>**: 9nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.5
    * **<color #00a2e8>Numbers of reads</color>**: 19549288


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|58/26|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Colchicine_0.009uM_Round-5_exp215.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Colchicine 0.009μM R05 exp215'>
{{:chemogenomics:cranks_plots:exp215.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:K:KIF15|KIF15]]|-5.11|<0.01|
|[[:human_genes:S:STMN1|STMN1]]|-4.93|<0.01|
|[[:human_genes:K:KNTC1|KNTC1]]|-4.42|<0.01|
|[[:human_genes:D:DLGAP5|DLGAP5]]|-4.35|<0.01|
|[[:human_genes:L:LRRC8A|LRRC8A]]|-3.91|<0.01|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-3.91|<0.01|
|[[:human_genes:A:ABCC1|ABCC1]]|-3.85|<0.01|
|[[:human_genes:D:DPH1|DPH1]]|-3.82|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-3.78|<0.01|
|[[:human_genes:V:VBP1|VBP1]]|-3.68|<0.01|
|[[:human_genes:M:MAPRE1|MAPRE1]]|-3.61|<0.01|
|[[:human_genes:G:GCLM|GCLM]]|-3.53|<0.01|
|[[:human_genes:T:TACC3|TACC3]]|-3.40|<0.01|
|[[:human_genes:X:XRN2|XRN2]]|-3.39|0.01|
|[[:human_genes:A:ASPM|ASPM]]|-3.27|<0.01|
|[[:human_genes:T:TUBB4B|TUBB4B]]|-3.13|<0.01|
|[[:human_genes:T:TMEM30A|TMEM30A]]|-3.12|<0.01|
|[[:human_genes:A:ATP8B2|ATP8B2]]|-3.10|<0.01|
|[[:human_genes:G:GTSE1|GTSE1]]|-3.07|<0.01|
|[[:human_genes:U:UFSP2|UFSP2]]|-3.07|<0.01|
|[[:human_genes:Q:QRICH1|QRICH1]]|-3.03|<0.01|
|[[:human_genes:M:MBNL1|MBNL1]]|-2.98|<0.01|
|[[:human_genes:R:RHOA|RHOA]]|-2.90|<0.01|
|[[:human_genes:Z:ZNF367|ZNF367]]|-2.80|<0.01|
|[[:human_genes:L:LUC7L2|LUC7L2]]|-2.79|<0.01|
|[[:human_genes:N:NAA60|NAA60]]|-2.74|<0.01|
|[[:human_genes:C:CNOT8|CNOT8]]|-2.71|<0.01|
|[[:human_genes:S:SKA1|SKA1]]|-2.69|<0.01|
|[[:human_genes:B:BICD2|BICD2]]|-2.68|0.01|
|[[:human_genes:S:SOX4|SOX4]]|-2.67|0.02|
|[[:human_genes:N:NUP107|NUP107]]|2.34|0.07|
|[[:human_genes:P:PRKAR1A|PRKAR1A]]|2.45|0.02|
|[[:human_genes:M:MCL1|MCL1]]|2.46|0.04|
|[[:human_genes:T:TIPRL|TIPRL]]|2.50|0.02|
|[[:human_genes:K:KIF4A|KIF4A]]|2.51|0.02|
|[[:human_genes:E:EPT1|EPT1]]|2.52|0.02|
|[[:human_genes:D:DNTTIP2|DNTTIP2]]|2.53|0.06|
|[[:human_genes:P:PEX12|PEX12]]|2.56|0.02|
|[[:human_genes:P:PIGF|PIGF]]|2.57|0.02|
|[[:human_genes:M:MED26|MED26]]|2.57|0.03|
|[[:human_genes:N:NDE1|NDE1]]|2.62|0.02|
|[[:human_genes:E:EIF3A|EIF3A]]|2.62|0.02|
|[[:human_genes:R:RFC1|RFC1]]|2.63|0.14|
|[[:human_genes:A:AHCYL1|AHCYL1]]|2.66|0.02|
|[[:human_genes:H:HSPE1|HSPE1]]|2.71|0.31|
|[[:human_genes:N:NUP133|NUP133]]|2.72|0.06|
|[[:human_genes:T:TMEM189-UBE2V1|TMEM189-UBE2V1]]|2.75|<0.01|
|[[:human_genes:D:DYNC1H1|DYNC1H1]]|2.79|0.31|
|[[:human_genes:P:PIP4K2A|PIP4K2A]]|2.91|0.02|
|[[:human_genes:P:PPP1CA|PPP1CA]]|2.92|<0.01|
|[[:human_genes:P:PSMC2|PSMC2]]|3.01|0.02|
|[[:human_genes:P:PPP1CC|PPP1CC]]|3.02|<0.01|
|[[:human_genes:P:PPP3CA|PPP3CA]]|3.09|<0.01|
|[[:human_genes:A:ATP1B3|ATP1B3]]|3.12|<0.01|
|[[:human_genes:T:TMEM189|TMEM189]]|3.28|<0.01|
|[[:human_genes:P:PPP3R1|PPP3R1]]|3.47|<0.01|
|[[:human_genes:A:ATP1A1|ATP1A1]]|4.19|<0.01|
|[[:human_genes:T:TMEM161B|TMEM161B]]|4.41|<0.01|
|[[:human_genes:A:ATAT1|ATAT1]]|4.70|<0.01|
|[[:human_genes:N:NUMA1|NUMA1]]|4.98|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|0.189|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.136|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|0.112|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|0.098|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp59|UMK57 1μM R01 exp59]]|0.085|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.083|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.07|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.066|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|0.065|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|0.063|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp103|Taxol 0.004μM R03 exp103]]|0.06|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|0.056|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.136|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.076|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.072|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|0.061|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|0.054|<button type="primary" size="small" modal="17">View</button>|
</modal>


<modal id="1" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp215_vs_exp228.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Combretastatin A4 0.002 to 0.003μM day4 R04 exp179">
{{:chemogenomics:comparison_plots:exp179_vs_exp215.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Vincristine 0.0005μM R04 exp190">
{{:chemogenomics:comparison_plots:exp190_vs_exp215.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Mdivi-1 15μM R05 exp217">
{{:chemogenomics:comparison_plots:exp215_vs_exp217.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Colchicine 0.009μM R05 exp215  vs  UMK57 1μM R01 exp59">
{{:chemogenomics:comparison_plots:exp215_vs_exp59.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Colchicine 0.009μM R05 exp215  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp215_vs_exp243.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Quercetin 20μM R08 exp517">
{{:chemogenomics:comparison_plots:exp215_vs_exp517.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Ixabepilone 0.004 to 0.005μM on day4 R04 exp184">
{{:chemogenomics:comparison_plots:exp184_vs_exp215.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Vinblastine 0.002μM R01 exp60">
{{:chemogenomics:comparison_plots:exp215_vs_exp60.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Colchicine 0.009μM R05 exp215  vs  UM131593 0.2μM R03 exp106">
{{:chemogenomics:comparison_plots:exp106_vs_exp215.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Taxol 0.004μM R03 exp103">
{{:chemogenomics:comparison_plots:exp103_vs_exp215.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Epothilone-B 0.0015μM R05 exp231">
{{:chemogenomics:comparison_plots:exp215_vs_exp231.png?nolink |}}
</modal>

<modal id="13" size="lg" title="BML-284 0.09μM R08 exp460  vs  Colchicine 0.009μM R05 exp215">
{{:chemogenomics:comparison_plots:exp215_vs_exp460.png?nolink |}}
</modal>

<modal id="14" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  Colchicine 0.009μM R05 exp215">
{{:chemogenomics:comparison_plots:exp215_vs_exp312.png?nolink |}}
</modal>

<modal id="15" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  Colchicine 0.009μM R05 exp215">
{{:chemogenomics:comparison_plots:exp215_vs_exp311.png?nolink |}}
</modal>

<modal id="16" size="lg" title="BI-6727 0.0125μM R03 exp97  vs  Colchicine 0.009μM R05 exp215">
{{:chemogenomics:comparison_plots:exp215_vs_exp97.png?nolink |}}
</modal>

<modal id="17" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Colchicine 0.009μM R05 exp215">
{{:chemogenomics:comparison_plots:exp215_vs_exp310.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|centrosome localization|61.22|DLGAP5,ASPM,BICD2|
|microtubule organizing center localization|61.22|DLGAP5,ASPM,BICD2|
|mitotic spindle organization|24.92|STMN1,DLGAP5,TACC3,RHOA|
|microtubule cytoskeleton organization involved in mitosis|22.94|STMN1,DLGAP5,MAPRE1,TACC3,RHOA|
|regulation of microtubule cytoskeleton organization|22.33|STMN1,MAPRE1,TACC3,RHOA,SKA1,BICD2|
|spindle organization|20.64|STMN1,DLGAP5,MAPRE1,TACC3,ASPM,RHOA|
|mitotic spindle|20.06|DLGAP5,MAPRE1,TACC3,ASPM,TUBB4B,SKA1|
|spindle pole|17.41|KNTC1,DLGAP5,MAPRE1,TACC3,ASPM|
|regulation of microtubule-based process|14.49|STMN1,MAPRE1,TACC3,RHOA,SKA1,BICD2|
|chromosome segregation|12.67|KNTC1,DLGAP5,MAPRE1,ASPM,RHOA,NAA60,SKA1|
|spindle|11.44|KIF15,KNTC1,DLGAP5,MAPRE1,TACC3,ASPM,TUBB4B,SKA1|
|tubulin binding|11.32|KIF15,STMN1,DLGAP5,VBP1,MAPRE1,GTSE1,SKA1|
|microtubule|10.91|KIF15,STMN1,KNTC1,MAPRE1,ASPM,TUBB4B,GTSE1,SKA1|
|mitotic cell cycle|9.83|KIF15,STMN1,KNTC1,DLGAP5,MAPRE1,TACC3,TUBB4B,GTSE1,RHOA,SKA1|
|microtubule cytoskeleton organization|8.78|STMN1,DLGAP5,MAPRE1,TACC3,ASPM,TUBB4B,RHOA,BICD2|
|microtubule-based process|8.35|KIF15,STMN1,DLGAP5,VBP1,MAPRE1,TACC3,ASPM,TUBB4B,GTSE1,RHOA,BICD2|
|cell division|8.19|STMN1,KNTC1,MAPRE1,TACC3,ASPM,RHOA,SKA1|
|mitotic cell cycle process|8.00|STMN1,KNTC1,DLGAP5,MAPRE1,TACC3,GTSE1,RHOA|
|cell cycle process|6.77|STMN1,KNTC1,DLGAP5,MAPRE1,TACC3,ASPM,GTSE1,RHOA,NAA60,SKA1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|ethanolaminephosphotransferase activity|552.33|PIGF,SELENOI|
|protein serine/threonine phosphatase complex|45.09|PPP3R1,PPP3CA,PPP1CC,PPP1CA|
|phosphatase complex|43.32|PPP3R1,PPP3CA,PPP1CC,PPP1CA|
</modal>