====== CoCl2 18μM R07 exp346 ======
==== Mechanism of Action ====
Hypoxia mimetic, inhibits VHL-HIF-1α interaction, induces HIF-1α accumulation
  * **<color #00a2e8>Class / Subclass 1:</color>** Environmental Stresses / Heavy Metal
  * **<color #00a2e8>Class / Subclass 2:</color>** Metabolism / Metabolic Enzyme Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Cobalt dichloride hexahydrate%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 7791-13-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/24643|24643]]
    * **<color #00a2e8>IUPAC:</color>** %%cobalt(2+);dichloride;hexahydrate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/2ClH.Co.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2
    * **<color #00a2e8>INCHI Key:</color>** GFHNAMRJFCEERV-UHFFFAOYSA-L
    * **<color #00a2e8>Molecular Weight:</color>** 237.93
    * **<color #00a2e8>Canonical SMILES:</color>** O.O.O.O.O.O.[Cl-].[Cl-].[Co+2]
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** Cl2CoH12O6
{{:chemogenomics:structures:chem-0204.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** 255599
    * **<color #00a2e8>Lot #:</color>** 04324JB
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>XRPD:</color>** Expected CoCl2 6H2O; found CoCl2 2H2O
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Cobalt_chloride
    * **<color #00a2e8>Min:</color>** -6.9301; **<color #00a2e8>Max:</color>** 88.2117
{{:chemogenomics:dose_response:dr_424.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |11.8286    |
| IC30  |16.0679    |
| IC40  |21.1851    |
| IC50  |27.8871    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 18µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 1.7
    * **<color #00a2e8>Numbers of reads</color>**: 20414333


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|34/37|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/CoCl2_18uM_Round-7_exp346.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='CoCl2 18μM R07 exp346'>
{{:chemogenomics:cranks_plots:exp346.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SLC39A9|SLC39A9]]|-4.33|<0.01|
|[[:human_genes:S:SLC30A1|SLC30A1]]|-3.31|<0.01|
|[[:human_genes:H:HIF1AN|HIF1AN]]|-3.05|<0.01|
|[[:human_genes:A:AP1G1|AP1G1]]|-2.95|<0.01|
|[[:human_genes:P:PACS1|PACS1]]|-2.89|0.03|
|[[:human_genes:H:HEXIM1|HEXIM1]]|-2.86|<0.01|
|[[:human_genes:A:ABCB7|ABCB7]]|-2.85|<0.01|
|[[:human_genes:S:SLC39A10|SLC39A10]]|-2.82|<0.01|
|[[:human_genes:S:SLC7A11|SLC7A11]]|-2.79|<0.01|
|[[:human_genes:M:MBNL1|MBNL1]]|-2.77|<0.01|
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-2.66|0.02|
|[[:human_genes:S:SH3GL1|SH3GL1]]|-2.66|0.03|
|[[:human_genes:T:TFRC|TFRC]]|-2.66|0.23|
|[[:human_genes:S:SLC35E1|SLC35E1]]|-2.64|0.01|
|[[:human_genes:I:IREB2|IREB2]]|-2.62|0.02|
|[[:human_genes:T:TCF4|TCF4]]|-2.60|0.02|
|[[:human_genes:C:C7orf55-LUC7L2|C7orf55-LUC7L2]]|-2.56|0.02|
|[[:human_genes:S:SLC10A7|SLC10A7]]|-2.55|0.02|
|[[:human_genes:S:SPEN|SPEN]]|-2.54|0.02|
|[[:human_genes:N:NUBP1|NUBP1]]|-2.52|0.03|
|[[:human_genes:R:RBM26|RBM26]]|-2.52|0.03|
|[[:human_genes:L:LARP7|LARP7]]|-2.52|0.02|
|[[:human_genes:C:CCDC22|CCDC22]]|-2.50|0.02|
|[[:human_genes:P:PSMB8|PSMB8]]|-2.47|0.02|
|[[:human_genes:U:UBE2A|UBE2A]]|-2.46|0.02|
|[[:human_genes:P:POLE3|POLE3]]|-2.42|0.03|
|[[:human_genes:R:RAB14|RAB14]]|-2.41|0.03|
|[[:human_genes:C:CYB561A3|CYB561A3]]|-2.40|0.05|
|[[:human_genes:N:NID1|NID1]]|-2.39|0.03|
|[[:human_genes:U:UBE2E1|UBE2E1]]|-2.37|0.03|
|[[:human_genes:T:TAMM41|TAMM41]]|2.42|0.02|
|[[:human_genes:P:PITRM1|PITRM1]]|2.46|0.02|
|[[:human_genes:C:CSNK2A3|CSNK2A3]]|2.46|0.04|
|[[:human_genes:M:MSL1|MSL1]]|2.54|0.03|
|[[:human_genes:S:SLC39A14|SLC39A14]]|2.57|0.01|
|[[:human_genes:M:MRPL23|MRPL23]]|2.60|0.01|
|[[:human_genes:M:MOGS|MOGS]]|2.69|0.01|
|[[:human_genes:M:MBTPS1|MBTPS1]]|2.69|0.03|
|[[:human_genes:L:LAP3|LAP3]]|2.74|<0.01|
|[[:human_genes:S:SEC16A|SEC16A]]|2.74|0.05|
|[[:human_genes:R:RABL6|RABL6]]|2.76|<0.01|
|[[:human_genes:S:STRAP|STRAP]]|2.77|<0.01|
|[[:human_genes:U:USO1|USO1]]|2.79|<0.01|
|[[:human_genes:P:PTPMT1|PTPMT1]]|2.82|0.02|
|[[:human_genes:U:UGP2|UGP2]]|2.86|<0.01|
|[[:human_genes:B:BACH2|BACH2]]|2.90|<0.01|
|[[:human_genes:I:IBA57|IBA57]]|2.91|<0.01|
|[[:human_genes:N:NAIF1|NAIF1]]|2.92|<0.01|
|[[:human_genes:R:RBM10|RBM10]]|3.04|<0.01|
|[[:human_genes:D:DNAJC13|DNAJC13]]|3.13|0.00|
|[[:human_genes:S:SCAP|SCAP]]|3.17|0.00|
|[[:human_genes:A:ATXN7L3|ATXN7L3]]|3.28|0.00|
|[[:human_genes:P:PYROXD1|PYROXD1]]|3.30|<0.01|
|[[:human_genes:P:PGM3|PGM3]]|3.41|0.00|
|[[:human_genes:S:SLC39A6|SLC39A6]]|3.84|0.00|
|[[:human_genes:M:MAT2B|MAT2B]]|4.11|0.00|
|[[:human_genes:T:TMEM165|TMEM165]]|4.36|0.00|
|[[:human_genes:A:ATP2C1|ATP2C1]]|4.50|0.00|
|[[:human_genes:S:SEC31A|SEC31A]]|4.75|0.00|
|[[:human_genes:S:SLC39A8|SLC39A8]]|8.50|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.079|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp532|TIC10 10μM R08 exp532]]|0.051|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|0.181|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|0.068|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|0.063|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.054|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.052|<button type="primary" size="small" modal="7">View</button>|
</modal>


<modal id="1" size="lg" title="CoCl2 18μM R07 exp346  vs  Hydroxyurea 100μM R07 exp329">
{{:chemogenomics:comparison_plots:exp329_vs_exp346.png?nolink |}}
</modal>

<modal id="2" size="lg" title="CoCl2 18μM R07 exp346  vs  TIC10 10μM R08 exp532">
{{:chemogenomics:comparison_plots:exp346_vs_exp532.png?nolink |}}
</modal>

<modal id="3" size="lg" title="ABT-702 5μM plus CoCl2 18μM R07 exp320  vs  CoCl2 18μM R07 exp346">
{{:chemogenomics:comparison_plots:exp320_vs_exp346.png?nolink |}}
</modal>

<modal id="4" size="lg" title="ABT-702 5μM plus Deferoxamine 11μM R07 exp321  vs  CoCl2 18μM R07 exp346">
{{:chemogenomics:comparison_plots:exp321_vs_exp346.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  CoCl2 18μM R07 exp346">
{{:chemogenomics:comparison_plots:exp346_vs_exp454.png?nolink |}}
</modal>

<modal id="6" size="lg" title="BML-284 0.09μM R08 exp460  vs  CoCl2 18μM R07 exp346">
{{:chemogenomics:comparison_plots:exp346_vs_exp460.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  CoCl2 18μM R07 exp346">
{{:chemogenomics:comparison_plots:exp346_vs_exp440.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|zinc ion transmembrane transporter activity|77.23|SLC39A9,SLC30A1,SLC39A10|
|intracellular zinc ion homeostasis|74.01|SLC39A9,SLC30A1,SLC39A10|
|zinc ion transmembrane transport|68.32|SLC39A9,SLC30A1,SLC39A10|
|zinc ion transport|65.79|SLC39A9,SLC30A1,SLC39A10|
|intracellular iron ion homeostasis|52.24|ABCB7,ATP6V0A2,TFRC,IREB2,NUBP1,CYB561A3|
|transition metal ion transmembrane transporter activity|43.32|SLC39A9,SLC30A1,SLC39A10|
|transition metal ion transport|31.50|SLC39A9,SLC30A1,ABCB7,SLC39A10,TFRC|
|intracellular monoatomic cation homeostasis|16.12|SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|inorganic ion homeostasis|15.81|SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|intracellular monoatomic ion homeostasis|15.77|SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|monoatomic cation homeostasis|13.65|SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|monoatomic ion homeostasis|13.40|SLC39A9,SLC30A1,ABCB7,SLC39A10,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|intracellular chemical homeostasis|13.38|SLC39A9,SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|cellular homeostasis|11.14|SLC39A9,SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
|active transmembrane transporter activity|10.77|SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,SLC35E1,SLC10A7,CYB561A3|
|chemical homeostasis|8.50|SLC39A9,SLC30A1,ABCB7,SLC39A10,SLC7A11,ATP6V0A2,TFRC,IREB2,SLC10A7,NUBP1,CCDC22,CYB561A3|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|cadmium ion transmembrane transport|221.05|SLC39A8,SLC39A14|
|cadmium ion transport|221.05|SLC39A8,SLC39A14|
|manganese ion transport|221.05|SLC39A8,ATP2C1,TMEM165,SLC39A14|
|manganese ion transmembrane transporter activity|207.24|ATP2C1,TMEM165,SLC39A14|
|iron import into cell|184.21|SLC39A8,SLC39A14|
|manganese ion transmembrane transport|184.21|SLC39A8,ATP2C1,TMEM165|
|monoatomic cation:bicarbonate symporter activity|138.16|SLC39A8,SLC39A6,SLC39A14|
|zinc ion transmembrane transporter activity|72.08|SLC39A8,SLC39A6,SLC39A14|
|intracellular zinc ion homeostasis|69.08|SLC39A8,SLC39A6,SLC39A14|
|transition metal ion transmembrane transporter activity|67.39|SLC39A8,ATP2C1,TMEM165,SLC39A6,SLC39A14|
|zinc ion transmembrane transport|63.77|SLC39A8,SLC39A6,SLC39A14|
|zinc ion transport|61.40|SLC39A8,SLC39A6,SLC39A14|
|bicarbonate transmembrane transporter activity|59.21|SLC39A8,SLC39A6,SLC39A14|
|bicarbonate transport|50.24|SLC39A8,SLC39A6,SLC39A14|
|COPII-coated vesicle budding|43.63|SEC31A,SCAP,SEC16A|
|ER to Golgi transport vesicle membrane|37.47|SEC31A,SCAP,USO1,SEC16A|
|protein N-linked glycosylation|32.04|SLC39A8,TMEM165,PGM3,MOGS|
|transition metal ion transport|29.40|SLC39A8,ATP2C1,TMEM165,SLC39A6,SLC39A14|
|COPII-coated ER to Golgi transport vesicle|24.84|SEC31A,SCAP,USO1,SEC16A|
</modal>