====== TRAIL 50ng/ml R00 exp36 ======
==== Mechanism of Action ====
TNF-related apoptosis-inducing ligand, TNF superfamily member, induces extrinsic apoptosis
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Other Receptor Agonist




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%N/A%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** N/A
    * **<color #00a2e8>PubChem CID:</color>** N/A
    * **<color #00a2e8>IUPAC:</color>** %%N/A%%
    * **<color #00a2e8>INCHI Name:</color>** N/A
    * **<color #00a2e8>INCHI Key:</color>** N/A
    * **<color #00a2e8>Molecular Weight:</color>** 19.6kDa
    * **<color #00a2e8>Canonical SMILES:</color>** N/A
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE
{{:chemogenomics:structures:chem-0019.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Peprotech
    * **<color #00a2e8>Catalog #:</color>** 310-04
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
Characterization data not available.
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Trail
    * **<color #00a2e8>Min:</color>** -9.0552; **<color #00a2e8>Max:</color>** 7.2598
{{:chemogenomics:dose_response:dr_278.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 50ng/mL
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 2.1
    * **<color #00a2e8>Numbers of reads</color>**: 13298540


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|60/122|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/TRAIL_50_ng_per_mL_Round-0_exp36.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='TRAIL 50ng/ml R00 exp36'>
{{:chemogenomics:cranks_plots:exp36.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:C:CNOT2|CNOT2]]|-4.56|<0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|-4.03|<0.01|
|[[:human_genes:D:DEPDC5|DEPDC5]]|-3.59|<0.01|
|[[:human_genes:Z:ZBTB1|ZBTB1]]|-3.44|<0.01|
|[[:human_genes:S:SZT2|SZT2]]|-3.39|<0.01|
|[[:human_genes:R:RNF19B|RNF19B]]|-3.35|<0.01|
|[[:human_genes:B:BCL11A|BCL11A]]|-3.26|<0.01|
|[[:human_genes:M:MBD3|MBD3]]|-3.20|<0.01|
|[[:human_genes:C:CNOT8|CNOT8]]|-3.15|<0.01|
|[[:human_genes:G:GLRX3|GLRX3]]|-3.12|<0.01|
|[[:human_genes:A:ARID4B|ARID4B]]|-3.09|<0.01|
|[[:human_genes:M:MIDN|MIDN]]|-3.03|<0.01|
|[[:human_genes:U:UBE2D3|UBE2D3]]|-3.02|<0.01|
|[[:human_genes:P:PRKAG1|PRKAG1]]|-3.02|<0.01|
|[[:human_genes:P:PCBP2|PCBP2]]|-2.97|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-2.93|<0.01|
|[[:human_genes:U:UBE2N|UBE2N]]|-2.88|<0.01|
|[[:human_genes:N:NPRL2|NPRL2]]|-2.83|<0.01|
|[[:human_genes:R:RB1CC1|RB1CC1]]|-2.79|<0.01|
|[[:human_genes:W:WDR20|WDR20]]|-2.77|<0.01|
|[[:human_genes:D:DKC1|DKC1]]|-2.73|0.06|
|[[:human_genes:X:XIAP|XIAP]]|-2.71|<0.01|
|[[:human_genes:R:RBM10|RBM10]]|-2.71|<0.01|
|[[:human_genes:S:SPEN|SPEN]]|-2.71|0.01|
|[[:human_genes:T:TRAF2|TRAF2]]|-2.68|0.01|
|[[:human_genes:R:RECQL5|RECQL5]]|-2.67|0.01|
|[[:human_genes:L:LEF1|LEF1]]|-2.65|<0.01|
|[[:human_genes:M:MTA2|MTA2]]|-2.62|<0.01|
|[[:human_genes:R:RHOH|RHOH]]|-2.60|<0.01|
|[[:human_genes:Z:ZBTB10|ZBTB10]]|-2.60|0.02|
|[[:human_genes:S:SAMD4B|SAMD4B]]|3.60|<0.01|
|[[:human_genes:R:RBM6|RBM6]]|3.71|<0.01|
|[[:human_genes:C:CLK3|CLK3]]|3.72|<0.01|
|[[:human_genes:H:HNRNPL|HNRNPL]]|3.73|<0.01|
|[[:human_genes:I:IKZF1|IKZF1]]|3.83|<0.01|
|[[:human_genes:E:EIF4A1|EIF4A1]]|3.83|<0.01|
|[[:human_genes:B:BTAF1|BTAF1]]|3.97|<0.01|
|[[:human_genes:G:G3BP1|G3BP1]]|4.03|<0.01|
|[[:human_genes:P:PTMA|PTMA]]|4.17|<0.01|
|[[:human_genes:S:SPOP|SPOP]]|4.64|<0.01|
|[[:human_genes:Z:ZC3HAV1|ZC3HAV1]]|4.77|<0.01|
|[[:human_genes:F:FLCN|FLCN]]|4.87|<0.01|
|[[:human_genes:F:FNIP1|FNIP1]]|4.87|<0.01|
|[[:human_genes:C:CDK6|CDK6]]|5.13|<0.01|
|[[:human_genes:T:TP53|TP53]]|5.29|<0.01|
|[[:human_genes:Z:ZNF217|ZNF217]]|5.45|<0.01|
|[[:human_genes:C:CREB1|CREB1]]|5.46|<0.01|
|[[:human_genes:C:CHD7|CHD7]]|5.56|<0.01|
|[[:human_genes:Z:ZBTB7A|ZBTB7A]]|5.92|<0.01|
|[[:human_genes:M:MTMR4|MTMR4]]|6.09|<0.01|
|[[:human_genes:I:IKZF2|IKZF2]]|6.20|<0.01|
|[[:human_genes:K:KHDRBS1|KHDRBS1]]|6.28|<0.01|
|[[:human_genes:E:ELAVL1|ELAVL1]]|6.43|<0.01|
|[[:human_genes:C:CCNC|CCNC]]|6.56|<0.01|
|[[:human_genes:C:CXorf38|CXorf38]]|7.25|<0.01|
|[[:human_genes:F:FBXO11|FBXO11]]|7.58|<0.01|
|[[:human_genes:T:TNFRSF10B|TNFRSF10B]]|7.62|<0.01|
|[[:human_genes:F:FADD|FADD]]|8.49|<0.01|
|[[:human_genes:C:CASP8|CASP8]]|8.49|<0.01|
|[[:human_genes:C:CASP3|CASP3]]|8.50|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|0.408|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|0.065|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|0.054|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|0.053|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|0.078|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|0.073|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|0.058|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|0.058|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|0.058|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.057|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|0.055|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.055|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp211|AICAR 240μM R05 exp211]]|0.054|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.052|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|0.052|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|0.051|<button type="primary" size="small" modal="16">View</button>|
</modal>


<modal id="1" size="lg" title="TRAIL 50ng/ml R00 exp36  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp36.png?nolink |}}
</modal>

<modal id="2" size="lg" title="TRAIL 50ng/ml R00 exp36  vs  Thapsigargin 0.005μM R07 exp407">
{{:chemogenomics:comparison_plots:exp36_vs_exp407.png?nolink |}}
</modal>

<modal id="3" size="lg" title="TRAIL 50ng/ml R00 exp36  vs  Tubacin 1.6μM R07 exp416">
{{:chemogenomics:comparison_plots:exp36_vs_exp416.png?nolink |}}
</modal>

<modal id="4" size="lg" title="TRAIL 50ng/ml R00 exp36  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp36_vs_exp38.png?nolink |}}
</modal>

<modal id="5" size="lg" title="3-Bromopyruvate 7μM R04 exp175  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp175_vs_exp36.png?nolink |}}
</modal>

<modal id="6" size="lg" title="MLN-4924 0.2μM R00 exp21  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp21_vs_exp36.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Lead acetate 2000μM R08 exp497  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp497.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Olaparib 4μM R08 exp512  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp512.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Bortezomib 0.005μM R00 exp6  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp6.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp440.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Lead acetate 2000μM R08 exp498 no dilution day6  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp498.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Quercetin 20μM R08 exp517  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp517.png?nolink |}}
</modal>

<modal id="13" size="lg" title="AICAR 240μM R05 exp211  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp211_vs_exp36.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Hydroxyurea 100μM R07 exp329  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp329_vs_exp36.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp352_vs_exp36.png?nolink |}}
</modal>

<modal id="16" size="lg" title="I-BRD9 4μM R07 exp365  vs  TRAIL 50ng/ml R00 exp36">
{{:chemogenomics:comparison_plots:exp36_vs_exp365.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|GATOR1 complex|592.18|NPRL3,NPRL2|
|Seh1-associated complex|253.79|NPRL3,DEPDC5,NPRL2|
|negative regulation of TORC1 signaling|72.22|NPRL3,DEPDC5,SZT2,UBE2N,NPRL2|
|cellular response to amino acid starvation|50.40|NPRL3,DEPDC5,SZT2,NPRL2|
|response to amino acid starvation|47.37|NPRL3,DEPDC5,SZT2,NPRL2|
|negative regulation of TOR signaling|44.86|NPRL3,DEPDC5,SZT2,UBE2N,NPRL2|
|regulation of TORC1 signaling|34.43|NPRL3,DEPDC5,SZT2,UBE2N,NPRL2|
|regulation of TOR signaling|21.93|NPRL3,DEPDC5,SZT2,UBE2N,NPRL2|
|protein polyubiquitination|14.51|RNF19B,UBE2D3,UBE2N,XIAP,TRAF2|
|cellular response to nutrient levels|12.76|NPRL3,DEPDC5,SZT2,PRKAG1,NPRL2|
|positive regulation of cellular catabolic process|11.35|CNOT2,NPRL3,DEPDC5,CNOT8,NPRL2,RB1CC1|
|regulation of cellular catabolic process|8.28|CNOT2,NPRL3,DEPDC5,CNOT8,NPRL2,RB1CC1,DKC1,RBM10,TRAF2|
|positive regulation of catabolic process|8.06|CNOT2,NPRL3,DEPDC5,RNF19B,CNOT8,NPRL2,RB1CC1|
|DNA metabolic process|7.45|ZBTB1,ARID4B,UBE2D3,PDS5B,UBE2N,DKC1,RECQL5,LEF1,MTA2|
|kinase binding|6.20|BCL11A,GLRX3,MIDN,PRKAG1,RB1CC1,XIAP,TRAF2,RHOH|
|negative regulation of transcription by RNA polymerase II|6.14|CNOT2,ZBTB1,BCL11A,MBD3,ARID4B,UBE2D3,RBM10,SPEN,LEF1,MTA2|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|death effector domain binding|368.47|CASP8,FADD|
|cysteine-type endopeptidase activity involved in apoptotic signaling pathway|276.35|CASP3,CASP8|
|glial cell apoptotic process|276.35|CASP3,TP53|
|CKM complex|276.35|CCNC,MED13|
|TRAIL-activated apoptotic signaling pathway|276.35|CASP8,FADD,TNFRSF10B|
|death-inducing signaling complex|207.26|CASP3,CASP8,FADD|
|death receptor binding|82.91|CASP3,CASP8,FADD|
|regulation of programmed necrotic cell death|55.27|CASP8,FADD,TP53|
|lymphocyte differentiation|13.62|FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1|
|mRNA binding|12.40|ELAVL1,KHDRBS1,TP53,G3BP1,EIF4A1,HNRNPL,SAMD4B|
|leukocyte differentiation|11.93|CASP8,FADD,ZBTB7A,CHD7,CREB1,TP53,CDK6,FNIP1,IKZF1|
|mononuclear cell differentiation|11.83|FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1|
|regulation of mRNA metabolic process|11.24|ELAVL1,KHDRBS1,ZBTB7A,ZC3HAV1,HNRNPL,SAMD4B|
|apoptotic signaling pathway|10.77|CASP3,CASP8,FADD,TNFRSF10B,TP53,FLCN|
|cellular response to external stimulus|10.17|CASP8,FADD,TNFRSF10B,TP53,FNIP1,FLCN|
|lymphocyte activation|9.55|CASP8,FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1|
|hemopoiesis|9.11|CASP3,CASP8,FADD,ZBTB7A,CHD7,CREB1,TP53,CDK6,FNIP1,FLCN,IKZF1|
|positive regulation of apoptotic process|7.94|CASP3,CASP8,FADD,TNFRSF10B,TP53,FNIP1,FLCN|
|leukocyte activation|7.58|CASP8,FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1|
</modal>