====== GSK-J4 1-1.25μM to day4 R07 exp363 ======
==== Mechanism of Action ====
JMJD3/KDM6B, UTX/KDM6A histone demethylase inhibitor (H3K27me2/me3)
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Epigenetic Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%GSK J4 HCl%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1797983-09-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/71729974|71729974]]
    * **<color #00a2e8>IUPAC:</color>** %%ethyl 3-[[2-pyridin-2-yl-6-(1,2,4,5-tetrahydro-3-benzazepin-3-yl)pyrimidin-4-yl]amino]propanoate;hydrochloride%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C24H27N5O2.ClH/c1-2-31-23(30)10-14-26-21-17-22(28-24(27-21)20-9-5-6-13-25-20)29-15-11-18-7-3-4-8-19(18)12-16-29;/h3-9,13,17H,2,10-12,14-16H2,1H3,(H,26,27,28);1H
    * **<color #00a2e8>INCHI Key:</color>** TYXWLTBYINKVNT-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 454
    * **<color #00a2e8>Canonical SMILES:</color>** CCOC(=O)CCNC1=CC(=NC(=N1)C2=CC=CC=N2)N3CCC4=CC=CC=C4CC3.Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C24H28ClN5O2
{{:chemogenomics:structures:chem-0215.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** SML0701
    * **<color #00a2e8>Lot #:</color>** 053M4601V
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C24H27N5O2 418.22375; found 418.22478
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** GSK-J4
    * **<color #00a2e8>Min:</color>** -0.8548; **<color #00a2e8>Max:</color>** 90.3053
{{:chemogenomics:dose_response:dr_430.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.5053    |
| IC30  |0.6250    |
| IC40  |0.7484    |
| IC50  |0.8887    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 1-1.25µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.7
    * **<color #00a2e8>Numbers of reads</color>**: 15134007


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/12|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/GSK-J4_1-1.25day4uM_Round-7_exp363.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='GSK-J4 1-1.25μM to day4 R07 exp363'>
{{:chemogenomics:cranks_plots:exp363.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:C:C6orf136|C6orf136]]|-3.51|<0.01|
|[[:human_genes:C:CLUH|CLUH]]|-3.23|<0.01|
|[[:human_genes:A:ADCK4|ADCK4]]|-2.90|0.03|
|[[:human_genes:M:MTF1|MTF1]]|-2.53|0.13|
|[[:human_genes:Z:ZRANB2|ZRANB2]]|-2.44|0.11|
|[[:human_genes:S:SLC27A4|SLC27A4]]|-2.44|0.11|
|[[:human_genes:T:TCF4|TCF4]]|-2.31|0.17|
|[[:human_genes:A:ADSL|ADSL]]|-2.24|0.53|
|[[:human_genes:P:PRKD2|PRKD2]]|-2.23|0.24|
|[[:human_genes:M:MNS1|MNS1]]|-2.20|0.54|
|[[:human_genes:D:DHX8|DHX8]]|-2.20|0.54|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.18|0.54|
|[[:human_genes:A:ABCB7|ABCB7]]|-2.16|0.31|
|[[:human_genes:U:UMPS|UMPS]]|-2.15|0.76|
|[[:human_genes:V:VGF|VGF]]|-2.14|0.49|
|[[:human_genes:A:ACKR2|ACKR2]]|-2.13|0.32|
|[[:human_genes:M:MS4A13|MS4A13]]|-2.12|0.84|
|[[:human_genes:R:RHOA|RHOA]]|-2.12|0.32|
|[[:human_genes:I:IVL|IVL]]|-2.05|0.67|
|[[:human_genes:R:RPE|RPE]]|-2.05|0.54|
|[[:human_genes:S:SART3|SART3]]|-2.05|0.76|
|[[:human_genes:S:SLC25A20|SLC25A20]]|-2.04|0.47|
|[[:human_genes:P:PCYT2|PCYT2]]|-2.00|0.67|
|[[:human_genes:S:SLC30A1|SLC30A1]]|-1.99|0.53|
|[[:human_genes:P:PRDM7|PRDM7]]|-1.98|0.62|
|[[:human_genes:M:MAPK4|MAPK4]]|-1.92|0.54|
|[[:human_genes:C:CLSPN|CLSPN]]|-1.90|0.76|
|[[:human_genes:T:TXNDC11|TXNDC11]]|-1.89|0.67|
|[[:human_genes:K:KIAA2022|KIAA2022]]|-1.88|0.67|
|[[:human_genes:G:GLRX3|GLRX3]]|-1.88|0.62|
|[[:human_genes:Z:ZFY|ZFY]]|2.04|0.21|
|[[:human_genes:D:DEPDC5|DEPDC5]]|2.05|0.21|
|[[:human_genes:M:MED1|MED1]]|2.06|0.38|
|[[:human_genes:T:TP53|TP53]]|2.07|0.26|
|[[:human_genes:T:TMED2|TMED2]]|2.07|0.20|
|[[:human_genes:Z:ZBED2|ZBED2]]|2.10|0.35|
|[[:human_genes:K:KCTD8|KCTD8]]|2.11|0.17|
|[[:human_genes:C:C19orf43|C19orf43]]|2.12|0.23|
|[[:human_genes:S:SRD5A3|SRD5A3]]|2.12|0.16|
|[[:human_genes:E:ELAVL1|ELAVL1]]|2.14|0.15|
|[[:human_genes:P:PUS7L|PUS7L]]|2.23|0.11|
|[[:human_genes:D:DNAJC3|DNAJC3]]|2.32|0.07|
|[[:human_genes:D:DPH6|DPH6]]|2.41|0.05|
|[[:human_genes:Z:ZNF827|ZNF827]]|2.45|0.07|
|[[:human_genes:K:KRTAP10-12|KRTAP10-12]]|2.46|0.14|
|[[:human_genes:D:DPH3|DPH3]]|2.49|0.03|
|[[:human_genes:T:TGFBR3|TGFBR3]]|2.49|0.12|
|[[:human_genes:D:DPH2|DPH2]]|2.51|0.06|
|[[:human_genes:N:NOB1|NOB1]]|2.52|0.03|
|[[:human_genes:F:FAF2|FAF2]]|2.52|0.06|
|[[:human_genes:V:VDAC2|VDAC2]]|2.56|0.03|
|[[:human_genes:A:ATP2B1|ATP2B1]]|2.62|0.04|
|[[:human_genes:R:RPS25|RPS25]]|2.66|0.02|
|[[:human_genes:S:SEC63|SEC63]]|2.81|<0.01|
|[[:human_genes:B:BAK1|BAK1]]|3.06|<0.01|
|[[:human_genes:D:DNAJC24|DNAJC24]]|3.19|<0.01|
|[[:human_genes:D:DPH5|DPH5]]|3.40|<0.01|
|[[:human_genes:S:STARD7|STARD7]]|3.53|<0.01|
|[[:human_genes:D:DPH1|DPH1]]|4.58|<0.01|
|[[:human_genes:S:SLC39A10|SLC39A10]]|5.79|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp362|GSK-J4 1μM R07 exp362]]|0.125|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|0.061|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|0.06|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp134|MS023 2μM R03 exp134]]|0.052|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.051|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|0.061|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp116|AICAR 240μM R03 exp116]]|0.054|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp274|Citral 50μM R06 exp274]]|0.052|<button type="primary" size="small" modal="8">View</button>|
</modal>


<modal id="1" size="lg" title="GSK-J4 1-1.25μM to day4 R07 exp363  vs  GSK-J4 1μM R07 exp362">
{{:chemogenomics:comparison_plots:exp362_vs_exp363.png?nolink |}}
</modal>

<modal id="2" size="lg" title="GSK-J4 1-1.25μM to day4 R07 exp363  vs  UM0129480 7μM R02 exp85">
{{:chemogenomics:comparison_plots:exp363_vs_exp85.png?nolink |}}
</modal>

<modal id="3" size="lg" title="GSK-J4 1-1.25μM to day4 R07 exp363  vs  UM0131023 0.05μM R05 exp248">
{{:chemogenomics:comparison_plots:exp248_vs_exp363.png?nolink |}}
</modal>

<modal id="4" size="lg" title="GSK-J4 1-1.25μM to day4 R07 exp363  vs  MS023 2μM R03 exp134">
{{:chemogenomics:comparison_plots:exp134_vs_exp363.png?nolink |}}
</modal>

<modal id="5" size="lg" title="GSK-J4 1-1.25μM to day4 R07 exp363  vs  GSK626616 14μM R08 exp485">
{{:chemogenomics:comparison_plots:exp363_vs_exp485.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Centrinone 0.5μM R07 exp343  vs  GSK-J4 1-1.25μM to day4 R07 exp363">
{{:chemogenomics:comparison_plots:exp343_vs_exp363.png?nolink |}}
</modal>

<modal id="7" size="lg" title="AICAR 240μM R03 exp116  vs  GSK-J4 1-1.25μM to day4 R07 exp363">
{{:chemogenomics:comparison_plots:exp116_vs_exp363.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Citral 50μM R06 exp274  vs  GSK-J4 1-1.25μM to day4 R07 exp363">
{{:chemogenomics:comparison_plots:exp274_vs_exp363.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|protein histidyl modification to diphthamide|442.11|DPH1,DPH5,DPH2,DPH3|
|peptidyl-histidine modification|315.79|DPH5,DPH2,DPH3,DPH6|
</modal>