====== GSK-J4 1.5μM R08 exp441 ======
==== Mechanism of Action ====
JMJD3/KDM6B, UTX/KDM6A histone demethylase inhibitor (H3K27me2/me3)
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Epigenetic Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%GSK J4 HCl%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1797983-09-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/71729974|71729974]]
    * **<color #00a2e8>IUPAC:</color>** %%ethyl 3-[[2-pyridin-2-yl-6-(1,2,4,5-tetrahydro-3-benzazepin-3-yl)pyrimidin-4-yl]amino]propanoate;hydrochloride%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C24H27N5O2.ClH/c1-2-31-23(30)10-14-26-21-17-22(28-24(27-21)20-9-5-6-13-25-20)29-15-11-18-7-3-4-8-19(18)12-16-29;/h3-9,13,17H,2,10-12,14-16H2,1H3,(H,26,27,28);1H
    * **<color #00a2e8>INCHI Key:</color>** TYXWLTBYINKVNT-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 454
    * **<color #00a2e8>Canonical SMILES:</color>** CCOC(=O)CCNC1=CC(=NC(=N1)C2=CC=CC=N2)N3CCC4=CC=CC=C4CC3.Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C24H28ClN5O2
{{:chemogenomics:structures:chem-0215.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** SML0701
    * **<color #00a2e8>Lot #:</color>** 053M4601V
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C24H27N5O2 418.22375; found 418.22478
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** GSK-J4
    * **<color #00a2e8>Min:</color>** -0.8548; **<color #00a2e8>Max:</color>** 90.3053
{{:chemogenomics:dose_response:dr_430.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.5053    |
| IC30  |0.6250    |
| IC40  |0.7484    |
| IC50  |0.8887    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 1.5µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 0.7
    * **<color #00a2e8>Numbers of reads</color>**: 17884362


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|1/58|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/GSK-J4_1.5uM_Round-8_exp441.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='GSK-J4 1.5μM R08 exp441'>
{{:chemogenomics:cranks_plots:exp441.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:Z:ZBTB7A|ZBTB7A]]|-3.12|<0.01|
|[[:human_genes:E:ESPL1|ESPL1]]|-3.03|0.41|
|[[:human_genes:N:NPIPB8|NPIPB8]]|-2.95|0.24|
|[[:human_genes:A:ATP6AP1|ATP6AP1]]|-2.78|0.12|
|[[:human_genes:C:CFAP45|CFAP45]]|-2.75|0.41|
|[[:human_genes:S:SCAF1|SCAF1]]|-2.65|0.41|
|[[:human_genes:O:OTUB1|OTUB1]]|-2.58|0.12|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.56|0.51|
|[[:human_genes:R:RPL38|RPL38]]|-2.49|0.24|
|[[:human_genes:B:BCAM|BCAM]]|-2.47|0.12|
|[[:human_genes:C:CPSF6|CPSF6]]|-2.46|0.12|
|[[:human_genes:D:DVL3|DVL3]]|-2.32|0.19|
|[[:human_genes:C:CABLES1|CABLES1]]|-2.29|0.31|
|[[:human_genes:C:CCDC146|CCDC146]]|-2.27|0.22|
|[[:human_genes:A:AURKB|AURKB]]|-2.26|0.51|
|[[:human_genes:R:RPS11|RPS11]]|-2.24|0.41|
|[[:human_genes:C:CCDC83|CCDC83]]|-2.23|0.27|
|[[:human_genes:M:MTRNR2L3|MTRNR2L3]]|-2.22|0.55|
|[[:human_genes:C:COL21A1|COL21A1]]|-2.22|0.27|
|[[:human_genes:P:PELP1|PELP1]]|-2.21|0.41|
|[[:human_genes:M:MEMO1|MEMO1]]|-2.19|0.24|
|[[:human_genes:I:ITK|ITK]]|-2.17|0.24|
|[[:human_genes:E:ESYT1|ESYT1]]|-2.16|0.24|
|[[:human_genes:M:MYL2|MYL2]]|-2.15|0.31|
|[[:human_genes:P:PARK7|PARK7]]|-2.14|0.25|
|[[:human_genes:C:CFLAR|CFLAR]]|-2.10|0.27|
|[[:human_genes:C:CNOT2|CNOT2]]|-2.07|0.29|
|[[:human_genes:I:IGBP1|IGBP1]]|-2.07|0.46|
|[[:human_genes:T:TMEM230|TMEM230]]|-2.07|0.41|
|[[:human_genes:L:LRR1|LRR1]]|-2.06|0.47|
|[[:human_genes:M:MARCH5|MARCH5]]|2.59|<0.01|
|[[:human_genes:T:TAMM41|TAMM41]]|2.61|<0.01|
|[[:human_genes:M:MRPS23|MRPS23]]|2.62|<0.01|
|[[:human_genes:T:TEFM|TEFM]]|2.66|<0.01|
|[[:human_genes:M:MBTPS2|MBTPS2]]|2.67|0.01|
|[[:human_genes:C:CEPT1|CEPT1]]|2.67|<0.01|
|[[:human_genes:T:TFAM|TFAM]]|2.70|0.02|
|[[:human_genes:M:MRP63|MRP63]]|2.72|0.02|
|[[:human_genes:C:CASP9|CASP9]]|2.75|<0.01|
|[[:human_genes:R:RPIA|RPIA]]|2.77|<0.01|
|[[:human_genes:K:KIAA0391|KIAA0391]]|2.79|<0.01|
|[[:human_genes:R:RPUSD4|RPUSD4]]|2.85|<0.01|
|[[:human_genes:M:MTPAP|MTPAP]]|2.86|<0.01|
|[[:human_genes:M:MRPL21|MRPL21]]|2.90|<0.01|
|[[:human_genes:T:TP53|TP53]]|2.93|<0.01|
|[[:human_genes:Y:YARS2|YARS2]]|2.93|<0.01|
|[[:human_genes:M:MRPS14|MRPS14]]|2.99|<0.01|
|[[:human_genes:M:MRPL23|MRPL23]]|3.05|<0.01|
|[[:human_genes:C:COX5A|COX5A]]|3.08|<0.01|
|[[:human_genes:G:GSTO1|GSTO1]]|3.16|0.00|
|[[:human_genes:D:DARS2|DARS2]]|3.20|<0.01|
|[[:human_genes:C:CYCS|CYCS]]|3.26|0.00|
|[[:human_genes:M:MRPS18A|MRPS18A]]|3.31|0.00|
|[[:human_genes:B:BAK1|BAK1]]|3.65|0.00|
|[[:human_genes:S:SUPV3L1|SUPV3L1]]|3.70|0.00|
|[[:human_genes:A:APAF1|APAF1]]|3.79|0.00|
|[[:human_genes:H:HSD17B10|HSD17B10]]|4.10|0.00|
|[[:human_genes:H:HCCS|HCCS]]|4.60|0.00|
|[[:human_genes:S:STARD7|STARD7]]|5.41|0.00|
|[[:human_genes:S:SLC39A10|SLC39A10]]|8.50|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.127|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|0.117|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|0.111|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp532|TIC10 10μM R08 exp532]]|0.111|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|0.097|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|0.096|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|0.093|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|0.066|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|0.061|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.061|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|0.057|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|0.055|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|0.053|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|0.052|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|0.052|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|0.052|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|0.052|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|0.119|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|0.098|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.098|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|0.088|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp474|CR131-b 0.005μM R08 exp474]]|0.082|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|0.067|<button type="primary" size="small" modal="23">View</button>|
|[[:results:exp211|AICAR 240μM R05 exp211]]|0.063|<button type="primary" size="small" modal="24">View</button>|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|0.06|<button type="primary" size="small" modal="25">View</button>|
|[[:results:exp116|AICAR 240μM R03 exp116]]|0.059|<button type="primary" size="small" modal="26">View</button>|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|0.057|<button type="primary" size="small" modal="27">View</button>|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|0.054|<button type="primary" size="small" modal="28">View</button>|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|0.053|<button type="primary" size="small" modal="29">View</button>|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|0.051|<button type="primary" size="small" modal="30">View</button>|
</modal>


<modal id="1" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Hydroxyurea 100μM R07 exp329">
{{:chemogenomics:comparison_plots:exp329_vs_exp441.png?nolink |}}
</modal>

<modal id="2" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Tanespimycin 14μM R08 exp527">
{{:chemogenomics:comparison_plots:exp441_vs_exp527.png?nolink |}}
</modal>

<modal id="3" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  ONC212 0.15μM R08 exp513">
{{:chemogenomics:comparison_plots:exp441_vs_exp513.png?nolink |}}
</modal>

<modal id="4" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  TIC10 10μM R08 exp532">
{{:chemogenomics:comparison_plots:exp441_vs_exp532.png?nolink |}}
</modal>

<modal id="5" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  PU-H71 1μM R08 exp515">
{{:chemogenomics:comparison_plots:exp441_vs_exp515.png?nolink |}}
</modal>

<modal id="6" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Mitomycin-C 0.06μM R08 exp503">
{{:chemogenomics:comparison_plots:exp441_vs_exp503.png?nolink |}}
</modal>

<modal id="7" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Thimerosal 0.85μM R08 exp529">
{{:chemogenomics:comparison_plots:exp441_vs_exp529.png?nolink |}}
</modal>

<modal id="8" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  UM0125461 0.74μM R02 exp84">
{{:chemogenomics:comparison_plots:exp441_vs_exp84.png?nolink |}}
</modal>

<modal id="9" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Pimelic-diphenylamide-106 5μM R00 exp28">
{{:chemogenomics:comparison_plots:exp28_vs_exp441.png?nolink |}}
</modal>

<modal id="10" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Hippuristanol 0.12μM R08 exp488">
{{:chemogenomics:comparison_plots:exp441_vs_exp488.png?nolink |}}
</modal>

<modal id="11" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  THZ1 0.06μM R08 exp531">
{{:chemogenomics:comparison_plots:exp441_vs_exp531.png?nolink |}}
</modal>

<modal id="12" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  JQ1 0.8μM R08 exp435">
{{:chemogenomics:comparison_plots:exp435_vs_exp441.png?nolink |}}
</modal>

<modal id="13" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  LLY-283 2.6μM R06 exp290">
{{:chemogenomics:comparison_plots:exp290_vs_exp441.png?nolink |}}
</modal>

<modal id="14" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  UM0129480 7μM R02 exp85">
{{:chemogenomics:comparison_plots:exp441_vs_exp85.png?nolink |}}
</modal>

<modal id="15" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Olaparib 4μM R08 exp512">
{{:chemogenomics:comparison_plots:exp441_vs_exp512.png?nolink |}}
</modal>

<modal id="16" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  iCRT14 30μM R08 exp492">
{{:chemogenomics:comparison_plots:exp441_vs_exp492.png?nolink |}}
</modal>

<modal id="17" size="lg" title="GSK-J4 1.5μM R08 exp441  vs  Hippuristanol 0.12μM R08 exp489 no dilution day6">
{{:chemogenomics:comparison_plots:exp441_vs_exp489.png?nolink |}}
</modal>

<modal id="18" size="lg" title="CI-1040 9.5μM R08 exp472  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp441_vs_exp472.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Atovaquone 15μM R08 exp451  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp441_vs_exp451.png?nolink |}}
</modal>

<modal id="20" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp440_vs_exp441.png?nolink |}}
</modal>

<modal id="21" size="lg" title="Deferoxamine 11μM R07 exp350  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp350_vs_exp441.png?nolink |}}
</modal>

<modal id="22" size="lg" title="CR131-b 0.005μM R08 exp474  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp441_vs_exp474.png?nolink |}}
</modal>

<modal id="23" size="lg" title="Etoposide 0.3μM R05 exp199  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp199_vs_exp441.png?nolink |}}
</modal>

<modal id="24" size="lg" title="AICAR 240μM R05 exp211  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp211_vs_exp441.png?nolink |}}
</modal>

<modal id="25" size="lg" title="Ammonium tetrathiomolybdate 10μM R08 exp448  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp441_vs_exp448.png?nolink |}}
</modal>

<modal id="26" size="lg" title="AICAR 240μM R03 exp116  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp116_vs_exp441.png?nolink |}}
</modal>

<modal id="27" size="lg" title="Doxorubicin 0.02μM R08 exp478  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp441_vs_exp478.png?nolink |}}
</modal>

<modal id="28" size="lg" title="Etoposide 1μM R00 exp19  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp19_vs_exp441.png?nolink |}}
</modal>

<modal id="29" size="lg" title="Actinomycin-D 0.001μM R00 exp3  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp3_vs_exp441.png?nolink |}}
</modal>

<modal id="30" size="lg" title="Benzoate 20000μM R08 exp456  vs  GSK-J4 1.5μM R08 exp441">
{{:chemogenomics:comparison_plots:exp441_vs_exp456.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c|331.58|APAF1,CYCS,CASP9|
|mitochondrial RNA processing|110.53|HSD17B10,SUPV3L1,RPUSD4,PRORP|
|mitochondrial RNA metabolic process|89.96|HSD17B10,SUPV3L1,DARS2,YARS2,RPUSD4,TFAM,TEFM|
|mitochondrial nucleoid|65.79|HSD17B10,SUPV3L1,PRORP,TFAM,TEFM|
|nucleoid|65.79|HSD17B10,SUPV3L1,PRORP,TFAM,TEFM|
|mitochondrial gene expression|55.26|HSD17B10,SUPV3L1,MRPS18A,DARS2,MRPL23,MRPS14,YARS2,MRPL21,RPUSD4,PRORP,MRPL57,TFAM,TEFM,MRPS23|
|mitochondrial translation|40.94|MRPS18A,DARS2,MRPL23,MRPS14,YARS2,MRPL21,MRPL57,MRPS23|
|mitochondrial ribosome|37.68|MRPS18A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23|
|organellar ribosome|37.68|MRPS18A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23|
|structural constituent of ribosome|22.11|MRPS18A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23|
|mitochondrial protein-containing complex|20.17|HSD17B10,SUPV3L1,MRPS18A,COX5A,MRPL23,MRPS14,MRPL21,PRORP,MRPL57,MRPS23|
|ribosomal subunit|19.06|MRPS18A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23|
|mitochondrial matrix|17.49|HSD17B10,SUPV3L1,MRPS18A,DARS2,MRPL23,MRPS14,YARS2,TP53,MRPL21,RPUSD4,PRORP,MRPL57,TFAM,TEFM,MRPS23|
|translation|12.28|MRPS18A,DARS2,MRPL23,MRPS14,YARS2,MRPL21,MRPL57,MRPS23|
|mitochondrial inner membrane|12.07|HCCS,MRPS18A,CYCS,COX5A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23,TAMM41|
|peptide biosynthetic process|11.48|MRPS18A,DARS2,MRPL23,MRPS14,YARS2,MRPL21,MRPL57,MRPS23|
|organelle inner membrane|10.84|HCCS,MRPS18A,CYCS,COX5A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23,TAMM41|
|mitochondrial membrane|10.19|STARD7,HCCS,BAK1,MRPS18A,CYCS,COX5A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23,TAMM41,MARCHF5|
|mitochondrial envelope|9.57|STARD7,HCCS,BAK1,MRPS18A,CYCS,COX5A,MRPL23,MRPS14,MRPL21,MRPL57,MRPS23,TAMM41,MARCHF5|
</modal>