====== Amiloride 100μM R08 exp447 ======
==== Mechanism of Action ====
Inhibits epithelial sodium channel (ENaC) and other Na+/H+ channels, also inhibits urokinase-type plasminogen activator (uPA)
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Ion Channel Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Amiloride hydrochloride%%
    * **<color #00a2e8>Synonyms:</color>** MK-870 hydrochloride
    * **<color #00a2e8>CAS #:</color>** 2016-88-8
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/16230|16230]]
    * **<color #00a2e8>IUPAC:</color>** %%3,5-diamino-6-chloro-N-(diaminomethylidene)pyrazine-2-carboxamide;hydrochloride%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C6H8ClN7O.ClH/c7-2-4(9)13-3(8)1(12-2)5(15)14-6(10)11;/h(H4,8,9,13)(H4,10,11,14,15);1H
    * **<color #00a2e8>INCHI Key:</color>** ACHKKGDWZVCSNH-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 266.09
    * **<color #00a2e8>Canonical SMILES:</color>** C1(=C(N=C(C(=N1)Cl)N)N)C(=O)N=C(N)N.Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C6H9Cl2N7O
{{:chemogenomics:structures:chem-0258.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-B0285A
    * **<color #00a2e8>Lot #:</color>** 14566
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C6H8ClN7O 230.05516; found 230.05522
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Amiloride
    * **<color #00a2e8>Min:</color>** -0.7865; **<color #00a2e8>Max:</color>** 67.7240
{{:chemogenomics:dose_response:dr_496.png?nolink&500 |}}
\\ 
\\ 
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |3.7720    |
| IC20  |16.2300    |
| IC30  |42.8000    |
| IC40  |94.7900    |
| IC50  |196.6000    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 100µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 2.5
    * **<color #00a2e8>Numbers of reads</color>**: 18416066


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|10/36|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Amiloride_100uM_Round-8_exp447.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Amiloride 100μM R08 exp447'>
{{:chemogenomics:cranks_plots:exp447.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ABCC4|ABCC4]]|-5.44|<0.01|
|[[:human_genes:V:VPS25|VPS25]]|-3.16|0.17|
|[[:human_genes:T:TSSC1|TSSC1]]|-3.11|<0.01|
|[[:human_genes:P:PRKAA1|PRKAA1]]|-3.08|<0.01|
|[[:human_genes:M:MTF2|MTF2]]|-3.05|<0.01|
|[[:human_genes:G:GOT1|GOT1]]|-2.83|<0.01|
|[[:human_genes:R:RIC8A|RIC8A]]|-2.76|0.01|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.72|0.01|
|[[:human_genes:T:TCF4|TCF4]]|-2.70|0.01|
|[[:human_genes:P:PIK3CA|PIK3CA]]|-2.55|0.03|
|[[:human_genes:F:FDPS|FDPS]]|-2.51|0.07|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-2.47|0.04|
|[[:human_genes:Z:ZSCAN12|ZSCAN12]]|-2.38|0.32|
|[[:human_genes:M:MSI2|MSI2]]|-2.38|0.07|
|[[:human_genes:G:GPR101|GPR101]]|-2.32|0.09|
|[[:human_genes:E:ESPL1|ESPL1]]|-2.31|0.30|
|[[:human_genes:P:PRKAG1|PRKAG1]]|-2.30|0.16|
|[[:human_genes:O:OR10G3|OR10G3]]|-2.27|0.22|
|[[:human_genes:D:DCUN1D1|DCUN1D1]]|-2.24|0.13|
|[[:human_genes:L:LACC1|LACC1]]|-2.21|0.14|
|[[:human_genes:P:PTPN6|PTPN6]]|-2.21|0.14|
|[[:human_genes:N:NNT|NNT]]|-2.17|0.22|
|[[:human_genes:G:GOLGA8H|GOLGA8H]]|-2.16|0.49|
|[[:human_genes:P:PRAMEF6|PRAMEF6]]|-2.16|0.16|
|[[:human_genes:U:UROD|UROD]]|-2.15|0.33|
|[[:human_genes:S:SIK2|SIK2]]|-2.15|0.22|
|[[:human_genes:T:TNIK|TNIK]]|-2.14|0.22|
|[[:human_genes:A:ACACA|ACACA]]|-2.14|0.22|
|[[:human_genes:N:NLRX1|NLRX1]]|-2.13|0.22|
|[[:human_genes:U:UBR4|UBR4]]|-2.13|0.30|
|[[:human_genes:P:PPP3CA|PPP3CA]]|2.41|0.03|
|[[:human_genes:P:PPA1|PPA1]]|2.43|0.04|
|[[:human_genes:N:NARS2|NARS2]]|2.45|0.02|
|[[:human_genes:P:PTAR1|PTAR1]]|2.46|0.04|
|[[:human_genes:M:MBNL1|MBNL1]]|2.46|0.02|
|[[:human_genes:U:UBE2G2|UBE2G2]]|2.48|0.04|
|[[:human_genes:A:AGPAT1|AGPAT1]]|2.48|0.06|
|[[:human_genes:C:CHKA|CHKA]]|2.49|0.02|
|[[:human_genes:Z:ZBTB18|ZBTB18]]|2.54|0.02|
|[[:human_genes:S:SHMT2|SHMT2]]|2.56|0.04|
|[[:human_genes:T:TRMT61A|TRMT61A]]|2.57|0.04|
|[[:human_genes:H:HNRNPH1|HNRNPH1]]|2.58|0.02|
|[[:human_genes:N:NDUFAF7|NDUFAF7]]|2.58|0.02|
|[[:human_genes:W:WDR66|WDR66]]|2.58|0.07|
|[[:human_genes:N:NFRKB|NFRKB]]|2.62|0.01|
|[[:human_genes:S:SLC7A1|SLC7A1]]|2.64|0.06|
|[[:human_genes:S:STARD7|STARD7]]|2.68|0.02|
|[[:human_genes:A:ADIPOR1|ADIPOR1]]|2.72|<0.01|
|[[:human_genes:C:CTDSP1|CTDSP1]]|2.87|<0.01|
|[[:human_genes:R:RAF1|RAF1]]|2.93|<0.01|
|[[:human_genes:W:WDR20|WDR20]]|2.97|<0.01|
|[[:human_genes:B:BEND3|BEND3]]|3.00|<0.01|
|[[:human_genes:L:LRRC63|LRRC63]]|3.01|0.18|
|[[:human_genes:R:RHOH|RHOH]]|3.05|<0.01|
|[[:human_genes:E:EIF4A1|EIF4A1]]|3.06|<0.01|
|[[:human_genes:S:SLC25A51|SLC25A51]]|3.35|<0.01|
|[[:human_genes:P:PIK3CD|PIK3CD]]|3.42|<0.01|
|[[:human_genes:A:ACSL4|ACSL4]]|4.00|<0.01|
|[[:human_genes:M:MDH1|MDH1]]|4.54|<0.01|
|[[:human_genes:S:SLC43A3|SLC43A3]]|7.16|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|0.093|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|0.085|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|0.079|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|0.073|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|0.07|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.066|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|0.066|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|0.063|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.062|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|0.06|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp134|MS023 2μM R03 exp134]]|0.06|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|0.06|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|0.059|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|0.057|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|0.057|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp274|Citral 50μM R06 exp274]]|0.056|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp359|FK-506 30μM R07 exp359]]|0.056|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp532|TIC10 10μM R08 exp532]]|0.055|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp534|Trientine 500μM R08 exp534]]|0.055|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|0.054|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp275|Citral 75μM R06 exp275]]|0.053|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|0.052|<button type="primary" size="small" modal="22">View</button>|
</modal>


<modal id="1" size="lg" title="Amiloride 100μM R08 exp447  vs  QNZ 0.01μM R08 exp439">
{{:chemogenomics:comparison_plots:exp439_vs_exp447.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Amiloride 100μM R08 exp447  vs  Rotenone 0.07μM R08 exp431">
{{:chemogenomics:comparison_plots:exp431_vs_exp447.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Amiloride 100μM R08 exp447  vs  ∆-9-Tetrahydrocannabinol 30μM R08 exp445">
{{:chemogenomics:comparison_plots:exp445_vs_exp447.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Amiloride 100μM R08 exp447  vs  ONC212 0.15μM R08 exp513">
{{:chemogenomics:comparison_plots:exp447_vs_exp513.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Amiloride 100μM R08 exp447  vs  MLN-4924 0.2μM R00 exp21">
{{:chemogenomics:comparison_plots:exp21_vs_exp447.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Amiloride 100μM R08 exp447  vs  Hippuristanol 0.12μM R08 exp488">
{{:chemogenomics:comparison_plots:exp447_vs_exp488.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Amiloride 100μM R08 exp447  vs  iCRT14 30μM R08 exp492">
{{:chemogenomics:comparison_plots:exp447_vs_exp492.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Amiloride 100μM R08 exp447  vs  Phenformin 20μM R05 exp212">
{{:chemogenomics:comparison_plots:exp212_vs_exp447.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Amiloride 100μM R08 exp447  vs  Aphidicolin 0.4μM R08 exp440">
{{:chemogenomics:comparison_plots:exp440_vs_exp447.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Amiloride 100μM R08 exp447  vs  Hippuristanol 0.12μM R08 exp489 no dilution day6">
{{:chemogenomics:comparison_plots:exp447_vs_exp489.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Amiloride 100μM R08 exp447  vs  MS023 2μM R03 exp134">
{{:chemogenomics:comparison_plots:exp134_vs_exp447.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Amiloride 100μM R08 exp447  vs  Thimerosal 0.85μM R08 exp529">
{{:chemogenomics:comparison_plots:exp447_vs_exp529.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Amiloride 100μM R08 exp447  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp447_vs_exp499.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Amiloride 100μM R08 exp447  vs  Trimetrexate 0.03μM R08 exp535">
{{:chemogenomics:comparison_plots:exp447_vs_exp535.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Amiloride 100μM R08 exp447  vs  Mubritinib 0.2μM R01 exp48">
{{:chemogenomics:comparison_plots:exp447_vs_exp48.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Amiloride 100μM R08 exp447  vs  Citral 50μM R06 exp274">
{{:chemogenomics:comparison_plots:exp274_vs_exp447.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Amiloride 100μM R08 exp447  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp359_vs_exp447.png?nolink |}}
</modal>

<modal id="18" size="lg" title="Amiloride 100μM R08 exp447  vs  TIC10 10μM R08 exp532">
{{:chemogenomics:comparison_plots:exp447_vs_exp532.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Amiloride 100μM R08 exp447  vs  Trientine 500μM R08 exp534">
{{:chemogenomics:comparison_plots:exp447_vs_exp534.png?nolink |}}
</modal>

<modal id="20" size="lg" title="Amiloride 100μM R08 exp447  vs  Bisphenol A 100μM R06 exp269">
{{:chemogenomics:comparison_plots:exp269_vs_exp447.png?nolink |}}
</modal>

<modal id="21" size="lg" title="Amiloride 100μM R08 exp447  vs  Citral 75μM R06 exp275">
{{:chemogenomics:comparison_plots:exp275_vs_exp447.png?nolink |}}
</modal>

<modal id="22" size="lg" title="Amiloride 100μM R08 exp447  vs  UM0131023 0.05μM R05 exp248">
{{:chemogenomics:comparison_plots:exp248_vs_exp447.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>