====== Hydroxychloroquine 30μM R08 exp490 ======
==== Mechanism of Action ====
Lysosomotropic agent, prevents sequestration of heme into hemozoin, forms toxic complex with heme, also inhibits TLR 7/9
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antimalarial
  * **<color #00a2e8>Class / Subclass 2:</color>** DNA Damage, Repair and Replication / Intercalating Agent
  * **<color #00a2e8>Class / Subclass 3:</color>** Proteostasis / Autophagy Inhibitor


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Hydroxychloroquine sulfate%%
    * **<color #00a2e8>Synonyms:</color>** HCQ sulfate
    * **<color #00a2e8>CAS #:</color>** 747-36-4
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/12947|12947]]
    * **<color #00a2e8>IUPAC:</color>** %%2-[4-[(7-chloroquinolin-4-yl)amino]pentyl-ethylamino]ethanol;sulfuric acid%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C18H26ClN3O.H2O4S/c1-3-22(11-12-23)10-4-5-14(2)21-17-8-9-20-18-13-15(19)6-7-16(17)18;1-5(2,3)4/h6-9,13-14,23H,3-5,10-12H2,1-2H3,(H,20,21);(H2,1,2,3,4)
    * **<color #00a2e8>INCHI Key:</color>** JCBIVZZPXRZKTI-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 434
    * **<color #00a2e8>Canonical SMILES:</color>** CCN(CCCC(C)NC1=C2C=CC(=CC2=NC=C1)Cl)CCO.OS(=O)(=O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C18H28ClN3O5S
{{:chemogenomics:structures:chem-0298.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Combi-Blocks
    * **<color #00a2e8>Catalog #:</color>** QA-8047
    * **<color #00a2e8>Lot #:</color>** B11244
</panel>


<panel title='Compound Characterization' collapsed='true'>
Characterization data not available.
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Hydroxychloroquine
    * **<color #00a2e8>Min:</color>** -11.2129; **<color #00a2e8>Max:</color>** 98.9777
{{:chemogenomics:dose_response:dr_545.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |18.4900    |
| IC20  |24.0900    |
| IC30  |28.7200    |
| IC40  |33.1700    |
| IC50  |37.8700    |
| IC60  |43.2200    |
| IC70  |49.9200    |
| IC80  |59.5200    |
| IC90  |77.5500    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 30µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.2
    * **<color #00a2e8>Numbers of reads</color>**: 16492093


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|9/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Hydroxychloroquine_30uM_Round-8_exp490.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Hydroxychloroquine 30μM R08 exp490'>
{{:chemogenomics:cranks_plots:exp490.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:O:ODC1|ODC1]]|-4.41|<0.01|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-3.20|<0.01|
|[[:human_genes:K:KAT7|KAT7]]|-3.02|<0.01|
|[[:human_genes:W:WDR81|WDR81]]|-2.94|0.01|
|[[:human_genes:R:RAB7A|RAB7A]]|-2.88|<0.01|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-2.61|0.03|
|[[:human_genes:U:UBA3|UBA3]]|-2.59|0.09|
|[[:human_genes:A:ARL1|ARL1]]|-2.51|0.05|
|[[:human_genes:S:SLC25A1|SLC25A1]]|-2.49|0.05|
|[[:human_genes:C:CDIPT|CDIPT]]|-2.47|0.21|
|[[:human_genes:A:ADIPOR2|ADIPOR2]]|-2.46|0.05|
|[[:human_genes:S:SLC39A9|SLC39A9]]|-2.43|0.05|
|[[:human_genes:M:MARCKSL1|MARCKSL1]]|-2.43|0.05|
|[[:human_genes:F:FIGNL2|FIGNL2]]|-2.38|0.06|
|[[:human_genes:C:COMMD10|COMMD10]]|-2.38|0.06|
|[[:human_genes:U:UHRF1|UHRF1]]|-2.38|0.17|
|[[:human_genes:C:CCDC22|CCDC22]]|-2.28|0.09|
|[[:human_genes:M:MGA|MGA]]|-2.28|0.09|
|[[:human_genes:D:DOT1L|DOT1L]]|-2.26|0.09|
|[[:human_genes:C:CNOT4|CNOT4]]|-2.25|0.09|
|[[:human_genes:C:COMMD5|COMMD5]]|-2.23|0.10|
|[[:human_genes:B:BTN2A1|BTN2A1]]|-2.20|0.26|
|[[:human_genes:P:PEX7|PEX7]]|-2.18|0.12|
|[[:human_genes:A:ANO4|ANO4]]|-2.17|0.21|
|[[:human_genes:C:C6orf118|C6orf118]]|-2.17|0.26|
|[[:human_genes:T:TAAR5|TAAR5]]|-2.16|0.13|
|[[:human_genes:F:FDPS|FDPS]]|-2.13|0.23|
|[[:human_genes:A:ATP2A2|ATP2A2]]|-2.11|0.73|
|[[:human_genes:T:TMEM189|TMEM189]]|-2.11|0.17|
|[[:human_genes:P:PROL1|PROL1]]|-2.07|0.19|
|[[:human_genes:C:CDC40|CDC40]]|1.91|0.55|
|[[:human_genes:A:ANKK1|ANKK1]]|1.92|0.55|
|[[:human_genes:R:RCC1|RCC1]]|1.93|0.79|
|[[:human_genes:M:MTHFD2|MTHFD2]]|1.94|0.55|
|[[:human_genes:T:TAC4|TAC4]]|1.95|0.55|
|[[:human_genes:U:USP39|USP39]]|1.95|0.79|
|[[:human_genes:S:SURF1|SURF1]]|1.96|0.55|
|[[:human_genes:D:DAGLA|DAGLA]]|1.96|0.55|
|[[:human_genes:F:FRS2|FRS2]]|1.96|0.55|
|[[:human_genes:T:TRIM44|TRIM44]]|1.99|0.55|
|[[:human_genes:C:CCDC9|CCDC9]]|2.00|0.55|
|[[:human_genes:T:TCEAL5|TCEAL5]]|2.01|0.55|
|[[:human_genes:E:EXTL1|EXTL1]]|2.03|0.55|
|[[:human_genes:F:FAM9B|FAM9B]]|2.04|0.55|
|[[:human_genes:S:SREBF2|SREBF2]]|2.05|0.69|
|[[:human_genes:N:NPRL2|NPRL2]]|2.06|0.55|
|[[:human_genes:S:SHMT2|SHMT2]]|2.07|0.55|
|[[:human_genes:I:ISY1|ISY1]]|2.10|0.71|
|[[:human_genes:T:TMEM63C|TMEM63C]]|2.12|0.43|
|[[:human_genes:N:NID1|NID1]]|2.14|0.43|
|[[:human_genes:C:CPA2|CPA2]]|2.15|0.43|
|[[:human_genes:N:NDUFA13|NDUFA13]]|2.17|0.55|
|[[:human_genes:N:NEK3|NEK3]]|2.17|0.43|
|[[:human_genes:S:SEC13|SEC13]]|2.18|0.69|
|[[:human_genes:C:CSNK2A1|CSNK2A1]]|2.22|0.43|
|[[:human_genes:P:PTGIS|PTGIS]]|2.24|0.43|
|[[:human_genes:Z:ZNF91|ZNF91]]|2.27|0.43|
|[[:human_genes:E:EIF2S1|EIF2S1]]|2.52|0.77|
|[[:human_genes:G:GTPBP4|GTPBP4]]|2.57|0.43|
|[[:human_genes:M:MAT2B|MAT2B]]|2.60|0.22|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.096|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|0.095|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|0.069|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|0.098|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp483|FTY720 3μM R08 exp483]]|0.082|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp453|B02 10μM R08 exp453]]|0.063|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp465|Cannabidiol 13μM R08 exp465]]|0.061|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp457|Bisphenol F 50μM R08 exp457]]|0.059|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|0.053|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp452|Azithromycin 100μM R08 exp452]]|0.052|<button type="primary" size="small" modal="10">View</button>|
</modal>


<modal id="1" size="lg" title="Hydroxychloroquine 30μM R08 exp490  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp490_vs_exp530.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Hydroxychloroquine 30μM R08 exp490  vs  NN-Dimethylsphingosine 2.5μM R08 exp508">
{{:chemogenomics:comparison_plots:exp490_vs_exp508.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Hydroxychloroquine 30μM R08 exp490  vs  Prochlorperazine 5.2μM R02 exp77">
{{:chemogenomics:comparison_plots:exp490_vs_exp77.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Chloroquine 32μM R08 exp470  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp470_vs_exp490.png?nolink |}}
</modal>

<modal id="5" size="lg" title="FTY720 3μM R08 exp483  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp483_vs_exp490.png?nolink |}}
</modal>

<modal id="6" size="lg" title="B02 10μM R08 exp453  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp453_vs_exp490.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Cannabidiol 13μM R08 exp465  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp465_vs_exp490.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Bisphenol F 50μM R08 exp457  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp457_vs_exp490.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp454_vs_exp490.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Azithromycin 100μM R08 exp452  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp452_vs_exp490.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>