====== Milciclib 2μM R08 exp502 ======
==== Mechanism of Action ====
Inhibits CDK2, also TRKA, CDK1, CDK4
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Cyclin Dependent Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / Kinase Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Milciclib%%
    * **<color #00a2e8>Synonyms:</color>** PHA-848125
    * **<color #00a2e8>CAS #:</color>** 802539-81-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/16718576|16718576]]
    * **<color #00a2e8>IUPAC:</color>** %%N,1,4,4-tetramethyl-8-[4-(4-methylpiperazin-1-yl)anilino]-5H-pyrazolo[4,3-h]quinazoline-3-carboxamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C25H32N8O/c1-25(2)14-16-15-27-24(29-20(16)22-19(25)21(23(34)26-3)30-32(22)5)28-17-6-8-18(9-7-17)33-12-10-31(4)11-13-33/h6-9,15H,10-14H2,1-5H3,(H,26,34)(H,27,28,29)
    * **<color #00a2e8>INCHI Key:</color>** RXZMYLDMFYNEIM-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 460.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC1(CC2=CN=C(N=C2C3=C1C(=NN3C)C(=O)NC)NC4=CC=C(C=C4)N5CCN(CC5)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C25H32N8O
{{:chemogenomics:structures:chem-0307.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-10424
    * **<color #00a2e8>Lot #:</color>** 05417
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C25H32N8O 461.27718; found 461.2785
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Milciclib
    * **<color #00a2e8>Min:</color>** -12.0561; **<color #00a2e8>Max:</color>** 94.9848
{{:chemogenomics:dose_response:dr_555.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |0.3312    |
| IC20  |0.6400    |
| IC30  |0.9915    |
| IC40  |1.4200    |
| IC50  |1.9730    |
| IC60  |2.7430    |
| IC70  |3.9270    |
| IC80  |6.0840    |
| IC90  |11.7500    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 2µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 0.3
    * **<color #00a2e8>Numbers of reads</color>**: 16227722


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|23/45|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Milciclib_2uM_Round-8_exp502.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Milciclib 2μM R08 exp502'>
{{:chemogenomics:cranks_plots:exp502.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:U:UBE2H|UBE2H]]|-5.61|<0.01|
|[[:human_genes:C:CLK3|CLK3]]|-5.21|<0.01|
|[[:human_genes:W:WDR26|WDR26]]|-4.58|<0.01|
|[[:human_genes:Y:YPEL5|YPEL5]]|-4.39|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-4.18|<0.01|
|[[:human_genes:U:UXS1|UXS1]]|-3.92|<0.01|
|[[:human_genes:P:PRKAR1A|PRKAR1A]]|-3.24|<0.01|
|[[:human_genes:S:SCAF1|SCAF1]]|-3.20|0.09|
|[[:human_genes:B:BRD1|BRD1]]|-3.20|<0.01|
|[[:human_genes:M:MAEA|MAEA]]|-3.02|<0.01|
|[[:human_genes:C:CSK|CSK]]|-3.01|<0.01|
|[[:human_genes:S:SOX4|SOX4]]|-3.00|0.01|
|[[:human_genes:R:RPSA|RPSA]]|-2.93|0.16|
|[[:human_genes:T:TCF12|TCF12]]|-2.92|<0.01|
|[[:human_genes:S:STAG2|STAG2]]|-2.68|0.01|
|[[:human_genes:K:KAT7|KAT7]]|-2.67|0.01|
|[[:human_genes:A:ATP6V0C|ATP6V0C]]|-2.61|0.37|
|[[:human_genes:Z:ZNF292|ZNF292]]|-2.58|0.01|
|[[:human_genes:G:GPX4|GPX4]]|-2.58|0.01|
|[[:human_genes:D:DERL2|DERL2]]|-2.53|0.04|
|[[:human_genes:A:ADRM1|ADRM1]]|-2.52|0.04|
|[[:human_genes:O:OTUB1|OTUB1]]|-2.50|0.04|
|[[:human_genes:R:RANBP1|RANBP1]]|-2.48|0.02|
|[[:human_genes:B:BCL2L1|BCL2L1]]|-2.46|0.03|
|[[:human_genes:D:DCAF16|DCAF16]]|-2.45|0.03|
|[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.41|0.33|
|[[:human_genes:G:GID8|GID8]]|-2.37|0.04|
|[[:human_genes:A:ADSS|ADSS]]|-2.36|0.18|
|[[:human_genes:V:VPS25|VPS25]]|-2.32|0.37|
|[[:human_genes:R:RARS|RARS]]|-2.30|0.27|
|[[:human_genes:P:PTPMT1|PTPMT1]]|2.66|0.05|
|[[:human_genes:K:KCTD5|KCTD5]]|2.69|<0.01|
|[[:human_genes:P:PRKACA|PRKACA]]|2.69|<0.01|
|[[:human_genes:A:AMBRA1|AMBRA1]]|2.81|<0.01|
|[[:human_genes:M:MOCS3|MOCS3]]|2.83|0.01|
|[[:human_genes:P:PPP2R4|PPP2R4]]|2.95|<0.01|
|[[:human_genes:S:SPOP|SPOP]]|3.00|0.00|
|[[:human_genes:P:PPP3R1|PPP3R1]]|3.05|0.00|
|[[:human_genes:R:RIMKLB|RIMKLB]]|3.08|0.00|
|[[:human_genes:X:XPR1|XPR1]]|3.11|0.00|
|[[:human_genes:C:CXorf56|CXorf56]]|3.14|0.00|
|[[:human_genes:U:USO1|USO1]]|3.19|0.00|
|[[:human_genes:D:DGKQ|DGKQ]]|3.22|0.00|
|[[:human_genes:H:HDAC3|HDAC3]]|3.23|0.00|
|[[:human_genes:P:PPP2CA|PPP2CA]]|3.30|0.00|
|[[:human_genes:N:N6AMT1|N6AMT1]]|3.30|<0.01|
|[[:human_genes:T:TIPRL|TIPRL]]|3.37|0.00|
|[[:human_genes:B:BAK1|BAK1]]|3.39|0.00|
|[[:human_genes:R:RFX7|RFX7]]|3.40|0.00|
|[[:human_genes:S:SMEK1|SMEK1]]|3.41|0.00|
|[[:human_genes:P:PPME1|PPME1]]|3.41|0.00|
|[[:human_genes:D:DGKD|DGKD]]|3.42|0.00|
|[[:human_genes:G:GNAS|GNAS]]|3.43|0.00|
|[[:human_genes:M:MBTPS2|MBTPS2]]|3.49|0.00|
|[[:human_genes:C:CYB5R4|CYB5R4]]|3.49|0.00|
|[[:human_genes:A:ATIC|ATIC]]|3.56|0.00|
|[[:human_genes:H:HDAC7|HDAC7]]|3.91|0.00|
|[[:human_genes:N:NCOR1|NCOR1]]|5.05|0.00|
|[[:human_genes:L:LCMT1|LCMT1]]|5.75|0.00|
|[[:human_genes:R:RB1|RB1]]|7.23|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|0.101|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|0.071|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|0.066|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|0.065|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|0.061|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp532|TIC10 10μM R08 exp532]]|0.06|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp59|UMK57 1μM R01 exp59]]|0.058|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|0.056|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|0.055|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|0.055|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|0.055|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|0.053|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|0.091|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|0.088|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|0.083|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|0.079|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|0.073|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.068|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.067|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp134|MS023 2μM R03 exp134]]|0.067|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.065|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|0.065|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|0.065|<button type="primary" size="small" modal="23">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.064|<button type="primary" size="small" modal="24">View</button>|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|0.064|<button type="primary" size="small" modal="25">View</button>|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|0.063|<button type="primary" size="small" modal="26">View</button>|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|0.06|<button type="primary" size="small" modal="27">View</button>|
|[[:results:exp359|FK-506 30μM R07 exp359]]|0.06|<button type="primary" size="small" modal="28">View</button>|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|0.06|<button type="primary" size="small" modal="29">View</button>|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|0.059|<button type="primary" size="small" modal="30">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.057|<button type="primary" size="small" modal="31">View</button>|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|0.057|<button type="primary" size="small" modal="32">View</button>|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|0.054|<button type="primary" size="small" modal="33">View</button>|
|[[:results:exp135|MS023 7μM R03 exp135]]|0.054|<button type="primary" size="small" modal="34">View</button>|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|0.052|<button type="primary" size="small" modal="35">View</button>|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|0.051|<button type="primary" size="small" modal="36">View</button>|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|0.051|<button type="primary" size="small" modal="37">View</button>|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|0.051|<button type="primary" size="small" modal="38">View</button>|
</modal>


<modal id="1" size="lg" title="Milciclib 2μM R08 exp502  vs  Staurosporine 0.02μM R08 exp524">
{{:chemogenomics:comparison_plots:exp502_vs_exp524.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Milciclib 2μM R08 exp502  vs  RO-3307 4.7μM R02 exp80">
{{:chemogenomics:comparison_plots:exp502_vs_exp80.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Milciclib 2μM R08 exp502  vs  UM0129480 7μM R02 exp85">
{{:chemogenomics:comparison_plots:exp502_vs_exp85.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Milciclib 2μM R08 exp502  vs  Palbociclib 1μM R07 exp382">
{{:chemogenomics:comparison_plots:exp382_vs_exp502.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Milciclib 2μM R08 exp502  vs  PU-H71 1μM R08 exp515">
{{:chemogenomics:comparison_plots:exp502_vs_exp515.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Milciclib 2μM R08 exp502  vs  TIC10 10μM R08 exp532">
{{:chemogenomics:comparison_plots:exp502_vs_exp532.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Milciclib 2μM R08 exp502  vs  UMK57 1μM R01 exp59">
{{:chemogenomics:comparison_plots:exp502_vs_exp59.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Milciclib 2μM R08 exp502  vs  Thimerosal 0.85μM R08 exp529">
{{:chemogenomics:comparison_plots:exp502_vs_exp529.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Milciclib 2μM R08 exp502  vs  SNS-032 15μM R08 exp443">
{{:chemogenomics:comparison_plots:exp443_vs_exp502.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Milciclib 2μM R08 exp502  vs  Tanespimycin 14μM R08 exp527">
{{:chemogenomics:comparison_plots:exp502_vs_exp527.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Milciclib 2μM R08 exp502  vs  Mitomycin-C 0.06μM R08 exp503">
{{:chemogenomics:comparison_plots:exp502_vs_exp503.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Milciclib 2μM R08 exp502  vs  iCRT14 30μM R08 exp492">
{{:chemogenomics:comparison_plots:exp492_vs_exp502.png?nolink |}}
</modal>

<modal id="13" size="lg" title="JQ1 0.8μM R08 exp435  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp435_vs_exp502.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Dorsomorphin 5μM R07 exp357  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp357_vs_exp502.png?nolink |}}
</modal>

<modal id="15" size="lg" title="CB-5083 0.4μM R08 exp468  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp468_vs_exp502.png?nolink |}}
</modal>

<modal id="16" size="lg" title="BVD-523 15μM R02 exp67  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp502_vs_exp67.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Hippuristanol 0.12μM R08 exp489 no dilution day6  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp489_vs_exp502.png?nolink |}}
</modal>

<modal id="18" size="lg" title="GSK626616 14μM R08 exp485  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp485_vs_exp502.png?nolink |}}
</modal>

<modal id="19" size="lg" title="BML-284 0.09μM R08 exp460  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp460_vs_exp502.png?nolink |}}
</modal>

<modal id="20" size="lg" title="MS023 2μM R03 exp134  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp134_vs_exp502.png?nolink |}}
</modal>

<modal id="21" size="lg" title="Hippuristanol 0.12μM R08 exp488  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp488_vs_exp502.png?nolink |}}
</modal>

<modal id="22" size="lg" title="Lead acetate 2000μM R08 exp497  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp497_vs_exp502.png?nolink |}}
</modal>

<modal id="23" size="lg" title="LY2090314 0.003μM R08 exp499  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp499_vs_exp502.png?nolink |}}
</modal>

<modal id="24" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp440_vs_exp502.png?nolink |}}
</modal>

<modal id="25" size="lg" title="LY2090314 0.003μM R08 exp500 no dilution day6  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp500_vs_exp502.png?nolink |}}
</modal>

<modal id="26" size="lg" title="Erlotinib 10μM R05 exp216  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp216_vs_exp502.png?nolink |}}
</modal>

<modal id="27" size="lg" title="2-Deoxy-D-glucose 800μM R05 exp214  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp214_vs_exp502.png?nolink |}}
</modal>

<modal id="28" size="lg" title="FK-506 30μM R07 exp359  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp359_vs_exp502.png?nolink |}}
</modal>

<modal id="29" size="lg" title="Lead acetate 2000μM R08 exp498 no dilution day6  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp498_vs_exp502.png?nolink |}}
</modal>

<modal id="30" size="lg" title="∆-9-Tetrahydrocannabinol 30μM R08 exp445  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp445_vs_exp502.png?nolink |}}
</modal>

<modal id="31" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp312_vs_exp502.png?nolink |}}
</modal>

<modal id="32" size="lg" title="Bleomycin 5μM R08 exp459  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp459_vs_exp502.png?nolink |}}
</modal>

<modal id="33" size="lg" title="Arsenate 40μM R06 exp264  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp264_vs_exp502.png?nolink |}}
</modal>

<modal id="34" size="lg" title="MS023 7μM R03 exp135  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp135_vs_exp502.png?nolink |}}
</modal>

<modal id="35" size="lg" title="6-Thio-2-deoxyguanosine 2μM R06 exp259  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp259_vs_exp502.png?nolink |}}
</modal>

<modal id="36" size="lg" title="Actinomycin-D 0.001μM R00 exp3  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp3_vs_exp502.png?nolink |}}
</modal>

<modal id="37" size="lg" title="Mdivi-1 15μM R05 exp217  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp217_vs_exp502.png?nolink |}}
</modal>

<modal id="38" size="lg" title="Doxorubicin 0.02μM R08 exp478  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp478_vs_exp502.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|intracellular protein-containing complex|6.54|WDR26,YPEL5,PRKAR1A,BRD1,MAEA,KAT7,DERL2,ADRM1,DCAF16,GID8|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|protein serine/threonine phosphatase complex|45.09|PPP4R3A,PPP2CA,PPP3R1,PTPA|
</modal>