====== Monensin 0.3μM R08 exp507 ======
==== Mechanism of Action ====
Polyether antibiotic, binds monovalent cations, Na+/H+ antiporter, blocks Golgi transport
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Membrane Ionophore



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Monensin sodium%%
    * **<color #00a2e8>Synonyms:</color>** Monensin A sodium salt
    * **<color #00a2e8>CAS #:</color>** 22373-78-0
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/23667299|23667299]]
    * **<color #00a2e8>IUPAC:</color>** %%sodium;(2S,3R,4S)-4-[(2S,5R,7S,8R,9S)-2-[(2R,5S)-5-ethyl-5-[(2R,3S,5R)-5-[(2S,3S,5R,6R)-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyloxan-2-yl]-3-methyloxolan-2-yl]oxolan-2-yl]-7-hydroxy-2,8-dimethyl-1,10-dioxaspiro[4.5]decan-9-yl]-3-methoxy-2-methylpentanoate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C36H62O11.Na/c1-10-34(31-20(3)16-26(43-31)28-19(2)15-21(4)36(41,18-37)46-28)12-11-27(44-34)33(8)13-14-35(47-33)17-25(38)22(5)30(45-35)23(6)29(42-9)24(7)32(39)40;/h19-31,37-38,41H,10-18H2,1-9H3,(H,39,40);/q;+1/p-1/t19-,20-,21+,22+,23-,24-,25-,26+,27+,28-,29+,30-,31+,33-,34-,35+,36-;/m0./s1
    * **<color #00a2e8>INCHI Key:</color>** XOIQMTLWECTKJL-FBZUZRIGSA-M
    * **<color #00a2e8>Molecular Weight:</color>** 692.9
    * **<color #00a2e8>Canonical SMILES:</color>** CCC1(CCC(O1)C2(CCC3(O2)CC(C(C(O3)C(C)C(C(C)C(=O)[O-])OC)C)O)C)C4C(CC(O4)C5C(CC(C(O5)(CO)O)C)C)C.[Na+]
    * **<color #00a2e8>Isomeric SMILES:</color>** CC[C@]1(CC[C@@H](O1)[C@@]2(CC[C@@]3(O2)C[C@@H]([C@H]([C@H](O3)[C@@H](C)[C@H]([C@H](C)C(=O)[O-])OC)C)O)C)[C@H]4[C@H](C[C@@H](O4)[C@@H]5[C@H](C[C@H]([C@@](O5)(CO)O)C)C)C.[Na+]
    * **<color #00a2e8>Molecular Formula:</color>** C36H61NaO11
{{:chemogenomics:structures:chem-0312.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-N0150
    * **<color #00a2e8>Lot #:</color>** 28765
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C36H62O11 693.41843; found 693.41958
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Monensin
    * **<color #00a2e8>Min:</color>** -4.4012; **<color #00a2e8>Max:</color>** 96.4158
{{:chemogenomics:dose_response:dr_467.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0433    |
| IC30  |0.0965    |
| IC40  |0.1929    |
| IC50  |0.3731    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 300nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 0.9
    * **<color #00a2e8>Numbers of reads</color>**: 16398044


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|19/5|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Monensin_0.3uM_Round-8_exp507.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Monensin 0.3μM R08 exp507'>
{{:chemogenomics:cranks_plots:exp507.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:T:TMEM189|TMEM189]]|-3.49|<0.01|
|[[:human_genes:A:ARL1|ARL1]]|-3.39|<0.01|
|[[:human_genes:C:C10orf76|C10orf76]]|-3.35|<0.01|
|[[:human_genes:U:UGCG|UGCG]]|-3.31|<0.01|
|[[:human_genes:A:AVL9|AVL9]]|-3.29|<0.01|
|[[:human_genes:A:ARFRP1|ARFRP1]]|-3.29|<0.01|
|[[:human_genes:A:APBA3|APBA3]]|-3.14|<0.01|
|[[:human_genes:C:COPB2|COPB2]]|-3.10|0.06|
|[[:human_genes:R:RAB14|RAB14]]|-3.00|<0.01|
|[[:human_genes:G:GPR107|GPR107]]|-2.97|<0.01|
|[[:human_genes:S:SYS1|SYS1]]|-2.93|0.02|
|[[:human_genes:D:DDX23|DDX23]]|-2.84|0.22|
|[[:human_genes:S:SPTLC2|SPTLC2]]|-2.82|<0.01|
|[[:human_genes:R:RAB1B|RAB1B]]|-2.78|0.02|
|[[:human_genes:P:PI4KB|PI4KB]]|-2.68|0.03|
|[[:human_genes:U:UXS1|UXS1]]|-2.68|0.01|
|[[:human_genes:K:KIAA1432|KIAA1432]]|-2.63|0.03|
|[[:human_genes:P:PCYT1A|PCYT1A]]|-2.46|0.03|
|[[:human_genes:T:TMEM189-UBE2V1|TMEM189-UBE2V1]]|-2.45|0.03|
|[[:human_genes:T:TBC1D23|TBC1D23]]|-2.45|0.03|
|[[:human_genes:A:AP5B1|AP5B1]]|-2.44|0.03|
|[[:human_genes:P:POLE|POLE]]|-2.38|0.25|
|[[:human_genes:C:COPZ1|COPZ1]]|-2.29|0.06|
|[[:human_genes:U:UFD1L|UFD1L]]|-2.28|0.17|
|[[:human_genes:C:CDIPT|CDIPT]]|-2.28|0.23|
|[[:human_genes:E:EFCAB2|EFCAB2]]|-2.27|0.07|
|[[:human_genes:P:PSMA7|PSMA7]]|-2.23|0.25|
|[[:human_genes:T:TREML1|TREML1]]|-2.22|0.14|
|[[:human_genes:C:COPB1|COPB1]]|-2.22|0.33|
|[[:human_genes:S:SCN9A|SCN9A]]|-2.20|0.10|
|[[:human_genes:N:NRAP|NRAP]]|2.11|0.21|
|[[:human_genes:T:TAZ|TAZ]]|2.12|0.42|
|[[:human_genes:S:SCUBE2|SCUBE2]]|2.12|0.16|
|[[:human_genes:C:COMP|COMP]]|2.13|0.16|
|[[:human_genes:C:CRYAB|CRYAB]]|2.13|0.25|
|[[:human_genes:S:SCO2|SCO2]]|2.15|0.24|
|[[:human_genes:M:MLLT10|MLLT10]]|2.16|0.15|
|[[:human_genes:I:ID3|ID3]]|2.19|0.14|
|[[:human_genes:D:DCAF15|DCAF15]]|2.20|0.16|
|[[:human_genes:Y:YARS2|YARS2]]|2.21|0.16|
|[[:human_genes:T:TRIAP1|TRIAP1]]|2.22|0.21|
|[[:human_genes:K:KLHL23|KLHL23]]|2.22|0.13|
|[[:human_genes:V:VDAC2|VDAC2]]|2.27|0.11|
|[[:human_genes:M:MRPS18A|MRPS18A]]|2.27|0.11|
|[[:human_genes:D:DDX39A|DDX39A]]|2.29|0.11|
|[[:human_genes:S:SPG21|SPG21]]|2.32|0.11|
|[[:human_genes:T:TPK1|TPK1]]|2.37|0.12|
|[[:human_genes:V:VARS2|VARS2]]|2.37|0.21|
|[[:human_genes:M:MRPS33|MRPS33]]|2.41|0.16|
|[[:human_genes:U:URM1|URM1]]|2.42|0.08|
|[[:human_genes:F:FAM58A|FAM58A]]|2.42|0.08|
|[[:human_genes:T:TMEM126B|TMEM126B]]|2.44|0.11|
|[[:human_genes:T:TRMT10A|TRMT10A]]|2.59|0.04|
|[[:human_genes:R:RTCB|RTCB]]|2.60|0.16|
|[[:human_genes:D:DDX1|DDX1]]|2.66|0.04|
|[[:human_genes:R:RPTOR|RPTOR]]|2.68|0.06|
|[[:human_genes:T:TMED10|TMED10]]|2.69|0.04|
|[[:human_genes:S:STT3A|STT3A]]|2.76|0.11|
|[[:human_genes:N:NDUFA2|NDUFA2]]|3.06|0.02|
|[[:human_genes:E:ENY2|ENY2]]|3.15|0.02|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|0.077|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|0.06|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.057|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|0.057|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|0.055|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|0.052|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp510|Nicotine 3000μM R08 exp510]]|0.051|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|0.07|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|0.062|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.061|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|0.059|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|0.057|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.056|<button type="primary" size="small" modal="13">View</button>|
</modal>


<modal id="1" size="lg" title="Monensin 0.3μM R08 exp507  vs  Vemurafenib 6.6μM R08 exp434">
{{:chemogenomics:comparison_plots:exp434_vs_exp507.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Monensin 0.3μM R08 exp507  vs  PU-H71 1μM R08 exp515">
{{:chemogenomics:comparison_plots:exp507_vs_exp515.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Monensin 0.3μM R08 exp507  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp507_vs_exp90.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Monensin 0.3μM R08 exp507  vs  Tanespimycin 14μM R08 exp527">
{{:chemogenomics:comparison_plots:exp507_vs_exp527.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Monensin 0.3μM R08 exp507  vs  Vemurafenib 6.6μM R02 exp89">
{{:chemogenomics:comparison_plots:exp507_vs_exp89.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Monensin 0.3μM R08 exp507  vs  Q15 1 to 2μM on day4 R04 exp164">
{{:chemogenomics:comparison_plots:exp164_vs_exp507.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Monensin 0.3μM R08 exp507  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp507_vs_exp510.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Ammonium tetrathiomolybdate 10μM R08 exp448  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp448_vs_exp507.png?nolink |}}
</modal>

<modal id="9" size="lg" title="JQ1 0.8μM R08 exp435  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp435_vs_exp507.png?nolink |}}
</modal>

<modal id="10" size="lg" title="BS-181 20μM R08 exp461  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp461_vs_exp507.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Clomiphene 4.4μM R02 exp68  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp507_vs_exp68.png?nolink |}}
</modal>

<modal id="12" size="lg" title="CFI-400945 25μM R08 exp469  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp469_vs_exp507.png?nolink |}}
</modal>

<modal id="13" size="lg" title="BH1 1μM R04 exp169  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp169_vs_exp507.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|COPI vesicle coat|212.42|COPB2,COPZ1,COPB1|
|COPI-coated vesicle membrane|136.56|COPB2,COPZ1,COPB1|
|COPI-coated vesicle|91.04|COPB2,COPZ1,COPB1|
|protein localization to Golgi apparatus|65.92|ARL1,ARFRP1,SYS1|
|intra-Golgi vesicle-mediated transport|61.67|COPB2,COPZ1,COPB1|
|Golgi-associated vesicle|28.97|COPB2,RAB14,COPZ1,COPB1|
|coated membrane|28.64|COPB2,AP5B1,COPZ1,COPB1|
|membrane coat|28.64|COPB2,AP5B1,COPZ1,COPB1|
|retrograde transport, endosome to Golgi|28.32|ARL1,ARFRP1,RIC1,TBC1D23|
|cytosolic transport|25.32|ARL1,ARFRP1,RAB14,SYS1,RIC1,TBC1D23|
|trans-Golgi network membrane|24.99|ARL1,ARFRP1,SYS1,RIC1|
|endosomal transport|16.48|ARL1,ARFRP1,RAB14,RIC1,TBC1D23,AP5B1|
|Golgi vesicle transport|16.22|ARFRP1,COPB2,RAB14,SYS1,RAB1B,COPZ1,COPB1|
|trans-Golgi network|15.29|ARL1,ARFRP1,RAB14,SYS1,RIC1,TBC1D23|
|Golgi apparatus subcompartment|12.26|ARL1,ARFRP1,RAB14,SYS1,UXS1,RIC1,TBC1D23|
|Golgi membrane|9.71|ARL1,UGCG,COPB2,RAB14,SYS1,RAB1B,PI4KB,RIC1,COPZ1,COPB1|
|intracellular protein transport|8.46|ARL1,ARFRP1,COPB2,RAB14,RAB1B,RIC1,COPZ1,UFD1,COPB1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|tRNA metabolic process|17.82|DDX1,RTCB,TRMT10A,URM1,VARS2,YARS2|
</modal>