====== Nicotine 3000μM R08 exp510 ======
==== Mechanism of Action ====
Agonist for nicotinic acetylcholine receptors (nAChRs)
  * **<color #00a2e8>Class / Subclass 1:</color>** Environmental Stresses / Psychoactive Drug
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / Other Receptor Agonist



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Nicotine%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 54-11-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/89594|89594]]
    * **<color #00a2e8>IUPAC:</color>** %%3-[(2S)-1-methylpyrrolidin-2-yl]pyridine%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C10H14N2/c1-12-7-3-5-10(12)9-4-2-6-11-8-9/h2,4,6,8,10H,3,5,7H2,1H3/t10-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** SNICXCGAKADSCV-JTQLQIEISA-N
    * **<color #00a2e8>Molecular Weight:</color>** 162.23
    * **<color #00a2e8>Canonical SMILES:</color>** CN1CCCC1C2=CN=CC=C2
    * **<color #00a2e8>Isomeric SMILES:</color>** CN1CCC[C@H]1C2=CN=CC=C2
    * **<color #00a2e8>Molecular Formula:</color>** C10H14N2
{{:chemogenomics:structures:chem-0315.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-B0638
    * **<color #00a2e8>Lot #:</color>** 15566
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C10H14N2 163.12297; found 163.12521
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Nicotine
    * **<color #00a2e8>Min:</color>** 25.7126; **<color #00a2e8>Max:</color>** 99.9532
{{:chemogenomics:dose_response:dr_617.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |1812.0000    |
| IC20  |2391.0000    |
| IC30  |2874.0000    |
| IC40  |3343.0000    |
| IC50  |3839.0000    |
| IC60  |4410.0000    |
| IC70  |5128.0000    |
| IC80  |6165.0000    |
| IC90  |8134.0000    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 3000µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.1
    * **<color #00a2e8>Numbers of reads</color>**: 15758352


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|17/5|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Nicotine_3000uM_Round-8_exp510.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Nicotine 3000μM R08 exp510'>
{{:chemogenomics:cranks_plots:exp510.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:F:FLVCR1|FLVCR1]]|-4.61|<0.01|
|[[:human_genes:A:ARL1|ARL1]]|-4.01|<0.01|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-3.96|<0.01|
|[[:human_genes:S:SOX4|SOX4]]|-3.43|<0.01|
|[[:human_genes:A:APBA3|APBA3]]|-3.14|<0.01|
|[[:human_genes:K:KAT7|KAT7]]|-2.92|<0.01|
|[[:human_genes:K:KDSR|KDSR]]|-2.72|0.01|
|[[:human_genes:B:BRD1|BRD1]]|-2.65|0.02|
|[[:human_genes:U:UNC50|UNC50]]|-2.59|0.03|
|[[:human_genes:S:SLC10A7|SLC10A7]]|-2.57|0.03|
|[[:human_genes:W:WDR20|WDR20]]|-2.56|0.05|
|[[:human_genes:K:KCNN4|KCNN4]]|-2.55|0.05|
|[[:human_genes:G:GPR107|GPR107]]|-2.55|0.03|
|[[:human_genes:R:REST|REST]]|-2.48|0.03|
|[[:human_genes:P:PPIL1|PPIL1]]|-2.47|0.10|
|[[:human_genes:C:COG3|COG3]]|-2.44|0.04|
|[[:human_genes:D:DPH2|DPH2]]|-2.42|0.06|
|[[:human_genes:C:CLN3|CLN3]]|-2.42|0.06|
|[[:human_genes:P:PHIP|PHIP]]|-2.39|0.05|
|[[:human_genes:C:CNOT8|CNOT8]]|-2.38|0.05|
|[[:human_genes:T:TARS|TARS]]|-2.37|0.44|
|[[:human_genes:S:SLC12A9|SLC12A9]]|-2.34|0.05|
|[[:human_genes:M:MARCH6|MARCH6]]|-2.30|0.06|
|[[:human_genes:S:SCRIB|SCRIB]]|-2.30|0.10|
|[[:human_genes:S:SYS1|SYS1]]|-2.30|0.18|
|[[:human_genes:C:CORO6|CORO6]]|-2.28|0.06|
|[[:human_genes:S:SSB|SSB]]|-2.28|0.06|
|[[:human_genes:S:SLC36A1|SLC36A1]]|-2.23|0.08|
|[[:human_genes:C:COG7|COG7]]|-2.21|0.08|
|[[:human_genes:R:RBM26|RBM26]]|-2.16|0.18|
|[[:human_genes:M:MBD3|MBD3]]|2.10|0.17|
|[[:human_genes:A:ARHGEF1|ARHGEF1]]|2.10|0.17|
|[[:human_genes:R:RPL18|RPL18]]|2.10|0.66|
|[[:human_genes:E:EXOSC6|EXOSC6]]|2.12|0.41|
|[[:human_genes:P:PGGT1B|PGGT1B]]|2.15|0.14|
|[[:human_genes:P:PNMAL2|PNMAL2]]|2.15|0.22|
|[[:human_genes:U:UBE3C|UBE3C]]|2.16|0.14|
|[[:human_genes:C:CCDC90B|CCDC90B]]|2.16|0.14|
|[[:human_genes:K:KPTN|KPTN]]|2.18|0.14|
|[[:human_genes:M:MBNL1|MBNL1]]|2.18|0.14|
|[[:human_genes:V:VEZF1|VEZF1]]|2.19|0.14|
|[[:human_genes:T:TNPO1|TNPO1]]|2.21|0.17|
|[[:human_genes:N:NOP58|NOP58]]|2.22|0.25|
|[[:human_genes:W:WDR26|WDR26]]|2.24|0.25|
|[[:human_genes:P:PITPNB|PITPNB]]|2.26|0.12|
|[[:human_genes:C:CMAS|CMAS]]|2.26|0.12|
|[[:human_genes:G:GLYR1|GLYR1]]|2.28|0.12|
|[[:human_genes:M:MCOLN1|MCOLN1]]|2.34|0.14|
|[[:human_genes:A:ASUN|ASUN]]|2.36|0.10|
|[[:human_genes:N:NAMPT|NAMPT]]|2.37|0.25|
|[[:human_genes:I:IPMK|IPMK]]|2.38|0.10|
|[[:human_genes:M:MDH1|MDH1]]|2.38|0.10|
|[[:human_genes:C:C12orf66|C12orf66]]|2.38|0.13|
|[[:human_genes:M:MTA2|MTA2]]|2.45|0.08|
|[[:human_genes:E:EIF3F|EIF3F]]|2.48|0.10|
|[[:human_genes:R:RIC8A|RIC8A]]|2.69|0.02|
|[[:human_genes:G:GNA13|GNA13]]|2.81|0.02|
|[[:human_genes:N:NPRL2|NPRL2]]|3.18|0.02|
|[[:human_genes:I:ITFG2|ITFG2]]|3.65|<0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|4.26|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp389|PF-06409577 20μM R07 exp389]]|0.064|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.056|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.051|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|0.051|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|0.084|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.082|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp452|Azithromycin 100μM R08 exp452]]|0.08|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp504|MK2206 4μM R08 exp504]]|0.07|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp483|FTY720 3μM R08 exp483]]|0.066|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|0.063|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|0.062|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|0.056|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|0.055|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp433|LJH685 50μM R08 exp433]]|0.054|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|0.054|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|0.054|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.053|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|0.052|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp453|B02 10μM R08 exp453]]|0.052|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp507|Monensin 0.3μM R08 exp507]]|0.051|<button type="primary" size="small" modal="20">View</button>|
</modal>


<modal id="1" size="lg" title="Nicotine 3000μM R08 exp510  vs  PF-06409577 20μM R07 exp389">
{{:chemogenomics:comparison_plots:exp389_vs_exp510.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Nicotine 3000μM R08 exp510  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp510_vs_exp90.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Nicotine 3000μM R08 exp510  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp510_vs_exp530.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Nicotine 3000μM R08 exp510  vs  Vemurafenib 6.6μM R02 exp89">
{{:chemogenomics:comparison_plots:exp510_vs_exp89.png?nolink |}}
</modal>

<modal id="5" size="lg" title="EPZ-5676 30μM R05 exp233  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp233_vs_exp510.png?nolink |}}
</modal>

<modal id="6" size="lg" title="BS-181 20μM R08 exp461  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp461_vs_exp510.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Azithromycin 100μM R08 exp452  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp452_vs_exp510.png?nolink |}}
</modal>

<modal id="8" size="lg" title="MK2206 4μM R08 exp504  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp504_vs_exp510.png?nolink |}}
</modal>

<modal id="9" size="lg" title="FTY720 3μM R08 exp483  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp483_vs_exp510.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp454_vs_exp510.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Momordin-Ic 10μM R08 exp506  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp506_vs_exp510.png?nolink |}}
</modal>

<modal id="12" size="lg" title="NN-Dimethylsphingosine 2.5μM R08 exp508  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp508_vs_exp510.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Chloroquine 32μM R08 exp470  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp470_vs_exp510.png?nolink |}}
</modal>

<modal id="14" size="lg" title="LJH685 50μM R08 exp433  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp433_vs_exp510.png?nolink |}}
</modal>

<modal id="15" size="lg" title="BVD-523 15μM R02 exp67  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp510_vs_exp67.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Clomiphene 4.4μM R02 exp68  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp510_vs_exp68.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Chlorpromazine 10μM R07 exp344  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp344_vs_exp510.png?nolink |}}
</modal>

<modal id="18" size="lg" title="CCT251545 20μM R07 exp326  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp326_vs_exp510.png?nolink |}}
</modal>

<modal id="19" size="lg" title="B02 10μM R08 exp453  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp453_vs_exp510.png?nolink |}}
</modal>

<modal id="20" size="lg" title="Monensin 0.3μM R08 exp507  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp507_vs_exp510.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|KICSTOR complex|552.30|ITFG2,KPTN|
|GATOR1 complex|552.30|NPRL3,NPRL2|
|negative regulation of TORC1 signaling|53.88|NPRL3,ITFG2,NPRL2,KPTN|
|cellular response to amino acid starvation|46.02|NPRL3,ITFG2,NPRL2,KPTN|
|response to amino acid starvation|43.32|NPRL3,ITFG2,NPRL2,KPTN|
|negative regulation of TOR signaling|33.47|NPRL3,ITFG2,NPRL2,KPTN|
|regulation of TORC1 signaling|25.69|NPRL3,ITFG2,NPRL2,KPTN|
</modal>