====== Nocodazole 0.2μM R02 exp64 ======
==== Mechanism of Action ====
Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Microtubule Poison
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Nocodazole%%
    * **<color #00a2e8>Synonyms:</color>** Oncodazole; R17934
    * **<color #00a2e8>CAS #:</color>** 31430-18-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/4122|4122]]
    * **<color #00a2e8>IUPAC:</color>** %%methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
    * **<color #00a2e8>INCHI Key:</color>** KYRVNWMVYQXFEU-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 301.32
    * **<color #00a2e8>Canonical SMILES:</color>** COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C14H11N3O3S
{{:chemogenomics:structures:chem-0013.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** M1404
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C14H11N3O3S 302.05939; found 302.05966
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Nocodazole
    * **<color #00a2e8>Min:</color>** -9.8754; **<color #00a2e8>Max:</color>** 86.3481
{{:chemogenomics:dose_response:dr_270.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0313    |
| IC30  |0.0403    |
| IC40  |0.0496    |
| IC50  |0.0601    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 02
    * **<color #00a2e8>Dose</color>**: 200nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 0.0
    * **<color #00a2e8>Numbers of reads</color>**: 9840623


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/63|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Nocodazole_0.2uM_Round-2_exp64.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Nocodazole 0.2μM R02 exp64'>
{{:chemogenomics:cranks_plots:exp64.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:E:ESPL1|ESPL1]]|-2.83|0.33|
|[[:human_genes:A:ATP6V1A|ATP6V1A]]|-2.78|0.27|
|[[:human_genes:C:COPG1|COPG1]]|-2.53|0.27|
|[[:human_genes:G:GOLGA8H|GOLGA8H]]|-2.45|0.67|
|[[:human_genes:H:HSPG2|HSPG2]]|-2.41|0.27|
|[[:human_genes:C:CNTRL|CNTRL]]|-2.39|0.27|
|[[:human_genes:S:SLC47A1|SLC47A1]]|-2.38|0.27|
|[[:human_genes:C:CCDC27|CCDC27]]|-2.32|0.39|
|[[:human_genes:Z:ZFAND5|ZFAND5]]|-2.32|0.29|
|[[:human_genes:Y:YLPM1|YLPM1]]|-2.31|0.39|
|[[:human_genes:P:PSMB3|PSMB3]]|-2.30|0.62|
|[[:human_genes:P:PAQR6|PAQR6]]|-2.29|0.27|
|[[:human_genes:U:UBE2E1|UBE2E1]]|-2.28|0.27|
|[[:human_genes:L:LIG1|LIG1]]|-2.28|0.67|
|[[:human_genes:W:WDR43|WDR43]]|-2.21|0.61|
|[[:human_genes:E:EME1|EME1]]|-2.18|0.29|
|[[:human_genes:S:SKIV2L2|SKIV2L2]]|-2.16|0.61|
|[[:human_genes:O:OR51Q1|OR51Q1]]|-2.13|0.41|
|[[:human_genes:L:LOC200726|LOC200726]]|-2.13|0.41|
|[[:human_genes:E:ECT2|ECT2]]|-2.12|0.41|
|[[:human_genes:U:UBE4A|UBE4A]]|-2.12|0.33|
|[[:human_genes:E:ERCC8|ERCC8]]|-2.10|0.35|
|[[:human_genes:S:SART1|SART1]]|-2.10|0.76|
|[[:human_genes:K:KANSL2|KANSL2]]|-2.08|0.42|
|[[:human_genes:R:RPL30|RPL30]]|-2.06|0.61|
|[[:human_genes:R:RFC1|RFC1]]|-2.06|0.76|
|[[:human_genes:I:INO80E|INO80E]]|-2.05|0.39|
|[[:human_genes:R:RNPC3|RNPC3]]|-2.04|0.42|
|[[:human_genes:D:DNM2|DNM2]]|-2.03|0.76|
|[[:human_genes:P:PSAP|PSAP]]|-2.02|0.42|
|[[:human_genes:Z:ZDHHC4|ZDHHC4]]|2.77|<0.01|
|[[:human_genes:A:AP5Z1|AP5Z1]]|2.82|<0.01|
|[[:human_genes:M:MPP6|MPP6]]|2.87|<0.01|
|[[:human_genes:G:GMPPB|GMPPB]]|2.88|0.04|
|[[:human_genes:S:SUMF2|SUMF2]]|2.93|<0.01|
|[[:human_genes:H:HIBADH|HIBADH]]|2.97|0.00|
|[[:human_genes:A:ACTB|ACTB]]|2.99|<0.01|
|[[:human_genes:T:TTYH3|TTYH3]]|3.10|<0.01|
|[[:human_genes:K:KBTBD2|KBTBD2]]|3.14|0.00|
|[[:human_genes:Z:ZNRF2|ZNRF2]]|3.18|0.00|
|[[:human_genes:M:MYL7|MYL7]]|3.23|0.00|
|[[:human_genes:H:HECW1|HECW1]]|3.24|0.00|
|[[:human_genes:T:TNRC18|TNRC18]]|3.31|0.00|
|[[:human_genes:Z:ZWILCH|ZWILCH]]|3.34|0.00|
|[[:human_genes:P:PELO|PELO]]|3.35|<0.01|
|[[:human_genes:I:IGFBP1|IGFBP1]]|3.46|0.00|
|[[:human_genes:B:BMPER|BMPER]]|3.49|0.00|
|[[:human_genes:H:HOXA11|HOXA11]]|3.64|0.00|
|[[:human_genes:M:MAD2L1BP|MAD2L1BP]]|3.79|0.00|
|[[:human_genes:A:AMZ1|AMZ1]]|3.96|0.00|
|[[:human_genes:M:MAD2L1|MAD2L1]]|4.02|0.00|
|[[:human_genes:C:CDC20|CDC20]]|4.33|<0.01|
|[[:human_genes:B:BUB1B|BUB1B]]|4.66|0.00|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|5.04|0.00|
|[[:human_genes:R:RBM14|RBM14]]|5.42|0.00|
|[[:human_genes:M:MAD1L1|MAD1L1]]|5.43|0.00|
|[[:human_genes:B:BUB3|BUB3]]|5.78|0.00|
|[[:human_genes:K:KNTC1|KNTC1]]|6.55|0.00|
|[[:human_genes:T:TRIP13|TRIP13]]|6.63|0.00|
|[[:human_genes:Z:ZW10|ZW10]]|7.05|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|0.221|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|0.197|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|0.19|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|0.18|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|0.077|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|0.217|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|0.212|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|0.185|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|0.184|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|0.1|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp17|DABN 20μM R00 exp17]]|0.078|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|0.054|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|0.052|<button type="primary" size="small" modal="13">View</button>|
</modal>


<modal id="1" size="lg" title="Nocodazole 0.2μM R02 exp64  vs  SB743921 0.001μM R05 exp244">
{{:chemogenomics:comparison_plots:exp244_vs_exp64.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Nocodazole 0.2μM R02 exp64  vs  Rotenone 2μM R00 exp33">
{{:chemogenomics:comparison_plots:exp33_vs_exp64.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Nocodazole 0.2μM R02 exp64  vs  Vinblastine 0.015μM R05 exp208">
{{:chemogenomics:comparison_plots:exp208_vs_exp64.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Nocodazole 0.2μM R02 exp64  vs  R-DABN 8μM R03 exp111">
{{:chemogenomics:comparison_plots:exp111_vs_exp64.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Nocodazole 0.2μM R02 exp64  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp64.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp64_vs_exp94.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp24_vs_exp64.png?nolink |}}
</modal>

<modal id="8" size="lg" title="DABN racemic mixture R03 exp93  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp64_vs_exp93.png?nolink |}}
</modal>

<modal id="9" size="lg" title="HMS-I1 10μM R06 exp256  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp256_vs_exp64.png?nolink |}}
</modal>

<modal id="10" size="lg" title="BI-6727 0.04μM R03 exp98  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp64_vs_exp98.png?nolink |}}
</modal>

<modal id="11" size="lg" title="DABN 20μM R00 exp17  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp17_vs_exp64.png?nolink |}}
</modal>

<modal id="12" size="lg" title="BI-2536 0.02μM R03 exp96  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp64_vs_exp96.png?nolink |}}
</modal>

<modal id="13" size="lg" title="GSK461364A 0.1μM R03 exp126  vs  Nocodazole 0.2μM R02 exp64">
{{:chemogenomics:comparison_plots:exp126_vs_exp64.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|mitotic spindle assembly checkpoint signaling|177.69|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|spindle assembly checkpoint signaling|177.69|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|mitotic spindle checkpoint signaling|177.69|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|negative regulation of mitotic sister chromatid segregation|160.49|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|negative regulation of mitotic sister chromatid separation|160.49|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|spindle checkpoint signaling|152.50|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,ZWILCH|
|negative regulation of chromosome segregation|150.76|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|negative regulation of chromosome separation|150.76|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|negative regulation of metaphase/anaphase transition of cell cycle|150.76|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|negative regulation of mitotic metaphase/anaphase transition|142.66|ZW10,TRIP13,KNTC1,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|negative regulation of sister chromatid segregation|142.66|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,ZWILCH|
|negative regulation of nuclear division|108.16|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,ZWILCH|
|regulation of mitotic sister chromatid segregation|93.87|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,MAD2L1,MAD2L1BP,ZWILCH|
|regulation of mitotic sister chromatid separation|85.78|ZW10,TRIP13,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH|
|regulation of chromosome separation|75.73|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH|
|regulation of mitotic metaphase/anaphase transition|68.32|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB|
|regulation of metaphase/anaphase transition of cell cycle|66.10|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB|
|regulation of sister chromatid segregation|59.04|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB|
|regulation of chromosome segregation|48.26|ZW10,TRIP13,KNTC1,BUB3,MAD1L1,BUB1B,CDC20,MAD2L1,MAD2L1BP,ZWILCH,ACTB|
</modal>