====== MK-1775 0.32μM R02 exp75 ======
==== Mechanism of Action ====
Inhibits Wee1, alleviates CDK inhibition
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 3:</color>** DNA Damage, Repair and Replication / Checkpoint Signaling Inhibitor


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Adavosertib%%
    * **<color #00a2e8>Synonyms:</color>** AZD1775; MK-1775
    * **<color #00a2e8>CAS #:</color>** 955365-80-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/24856436|24856436]]
    * **<color #00a2e8>IUPAC:</color>** %%1-[6-(2-hydroxypropan-2-yl)pyridin-2-yl]-6-[4-(4-methylpiperazin-1-yl)anilino]-2-prop-2-enylpyrazolo[3,4-d]pyrimidin-3-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C27H32N8O2/c1-5-13-34-25(36)21-18-28-26(29-19-9-11-20(12-10-19)33-16-14-32(4)15-17-33)31-24(21)35(34)23-8-6-7-22(30-23)27(2,3)37/h5-12,18,37H,1,13-17H2,2-4H3,(H,28,29,31)
    * **<color #00a2e8>INCHI Key:</color>** BKWJAKQVGHWELA-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 500.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC(C)(C1=NC(=CC=C1)N2C3=NC(=NC=C3C(=O)N2CC=C)NC4=CC=C(C=C4)N5CCN(CC5)C)O
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C27H32N8O2
{{:chemogenomics:structures:chem-0051.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Repare Therapeutics
    * **<color #00a2e8>Catalog #:</color>** Unknown
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C27H32N8O2 501.2721; found 501.27254
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** MK-1775
    * **<color #00a2e8>Min:</color>** -5.3402; **<color #00a2e8>Max:</color>** 90.1692
{{:chemogenomics:dose_response:dr_17.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.2773    |
| IC30  |0.3795    |
| IC40  |0.4907    |
| IC50  |0.6212    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 02
    * **<color #00a2e8>Dose</color>**: 320nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.9
    * **<color #00a2e8>Numbers of reads</color>**: 14663165


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|38/29|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/MK-1775_0.32uM_Round-2_exp75.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='MK-1775 0.32μM R02 exp75'>
{{:chemogenomics:cranks_plots:exp75.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:P:PRPS1|PRPS1]]|-3.80|<0.01|
|[[:human_genes:M:MKLN1|MKLN1]]|-3.54|<0.01|
|[[:human_genes:R:RNASEH2A|RNASEH2A]]|-3.52|<0.01|
|[[:human_genes:P:PKMYT1|PKMYT1]]|-3.42|<0.01|
|[[:human_genes:U:UBE2H|UBE2H]]|-3.37|<0.01|
|[[:human_genes:Z:ZNF445|ZNF445]]|-3.34|<0.01|
|[[:human_genes:W:WEE1|WEE1]]|-3.31|0.05|
|[[:human_genes:M:MSI2|MSI2]]|-3.08|<0.01|
|[[:human_genes:S:SMEK1|SMEK1]]|-3.01|<0.01|
|[[:human_genes:S:SPEN|SPEN]]|-2.88|<0.01|
|[[:human_genes:W:WDR26|WDR26]]|-2.84|0.01|
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.78|0.29|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.77|0.29|
|[[:human_genes:F:FZR1|FZR1]]|-2.74|<0.01|
|[[:human_genes:K:KPNA6|KPNA6]]|-2.72|0.01|
|[[:human_genes:L:LCMT1|LCMT1]]|-2.71|<0.01|
|[[:human_genes:A:ARL14EP|ARL14EP]]|-2.70|<0.01|
|[[:human_genes:R:RNASEH2B|RNASEH2B]]|-2.68|<0.01|
|[[:human_genes:R:RIF1|RIF1]]|-2.66|<0.01|
|[[:human_genes:T:TMEM208|TMEM208]]|-2.65|0.01|
|[[:human_genes:C:CNOT3|CNOT3]]|-2.64|0.03|
|[[:human_genes:Y:YWHAE|YWHAE]]|-2.62|0.01|
|[[:human_genes:K:KPNA4|KPNA4]]|-2.61|0.01|
|[[:human_genes:Z:ZNF598|ZNF598]]|-2.59|0.01|
|[[:human_genes:Z:ZMYND19|ZMYND19]]|-2.58|0.01|
|[[:human_genes:N:NFRKB|NFRKB]]|-2.57|0.01|
|[[:human_genes:I:IVNS1ABP|IVNS1ABP]]|-2.55|0.01|
|[[:human_genes:C:CD52|CD52]]|-2.51|0.01|
|[[:human_genes:P:PPHLN1|PPHLN1]]|-2.50|0.01|
|[[:human_genes:T:TRIP12|TRIP12]]|-2.49|0.01|
|[[:human_genes:M:MRPL49|MRPL49]]|2.38|0.06|
|[[:human_genes:F:FBXL12|FBXL12]]|2.41|0.03|
|[[:human_genes:A:ANP32E|ANP32E]]|2.41|0.15|
|[[:human_genes:F:FBXO42|FBXO42]]|2.42|0.03|
|[[:human_genes:E:EPC1|EPC1]]|2.43|0.05|
|[[:human_genes:N:NGRN|NGRN]]|2.47|0.02|
|[[:human_genes:L:LLGL1|LLGL1]]|2.51|0.02|
|[[:human_genes:H:HMGXB3|HMGXB3]]|2.63|0.08|
|[[:human_genes:Z:ZDHHC17|ZDHHC17]]|2.65|<0.01|
|[[:human_genes:N:NBEAL1|NBEAL1]]|2.65|0.04|
|[[:human_genes:F:FARSA|FARSA]]|2.68|0.12|
|[[:human_genes:C:CCNL1|CCNL1]]|2.72|<0.01|
|[[:human_genes:D:DDI2|DDI2]]|2.78|<0.01|
|[[:human_genes:L:LRR1|LRR1]]|2.80|0.09|
|[[:human_genes:P:PTPN2|PTPN2]]|2.81|<0.01|
|[[:human_genes:C:CHD2|CHD2]]|2.82|<0.01|
|[[:human_genes:C:CEBPG|CEBPG]]|2.85|<0.01|
|[[:human_genes:E:EIF4G1|EIF4G1]]|2.89|<0.01|
|[[:human_genes:P:PRMT3|PRMT3]]|2.96|<0.01|
|[[:human_genes:D:DCUN1D1|DCUN1D1]]|3.08|0.00|
|[[:human_genes:R:RFC1|RFC1]]|3.49|0.10|
|[[:human_genes:F:FAM122A|FAM122A]]|3.60|0.00|
|[[:human_genes:E:EIF4B|EIF4B]]|3.77|0.00|
|[[:human_genes:C:CCNA2|CCNA2]]|4.00|0.00|
|[[:human_genes:S:SKP2|SKP2]]|4.63|0.00|
|[[:human_genes:E:EIF4A1|EIF4A1]]|4.65|0.00|
|[[:human_genes:S:SLFN11|SLFN11]]|5.16|0.00|
|[[:human_genes:C:CDK2|CDK2]]|5.42|0.00|
|[[:human_genes:G:GCN1L1|GCN1L1]]|6.17|0.00|
|[[:human_genes:E:EIF2AK4|EIF2AK4]]|6.27|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|0.09|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|0.079|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|0.055|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|0.052|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|0.068|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|0.056|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|0.056|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.053|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp359|FK-506 30μM R07 exp359]]|0.052|<button type="primary" size="small" modal="9">View</button>|
</modal>


<modal id="1" size="lg" title="MK-1775 0.32μM R02 exp75  vs  Pterostilbene 16μM R02 exp78">
{{:chemogenomics:comparison_plots:exp75_vs_exp78.png?nolink |}}
</modal>

<modal id="2" size="lg" title="MK-1775 0.32μM R02 exp75  vs  Resveratrol 16μM R03 exp147">
{{:chemogenomics:comparison_plots:exp147_vs_exp75.png?nolink |}}
</modal>

<modal id="3" size="lg" title="MK-1775 0.32μM R02 exp75  vs  Olaparib 4μM R08 exp512">
{{:chemogenomics:comparison_plots:exp512_vs_exp75.png?nolink |}}
</modal>

<modal id="4" size="lg" title="MK-1775 0.32μM R02 exp75  vs  Trimetrexate 0.03μM R08 exp535">
{{:chemogenomics:comparison_plots:exp535_vs_exp75.png?nolink |}}
</modal>

<modal id="5" size="lg" title="3-Bromopyruvate 7μM R04 exp175  vs  MK-1775 0.32μM R02 exp75">
{{:chemogenomics:comparison_plots:exp175_vs_exp75.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Diepoxybutane 3μM R07 exp354  vs  MK-1775 0.32μM R02 exp75">
{{:chemogenomics:comparison_plots:exp354_vs_exp75.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Erlotinib 10μM R05 exp216  vs  MK-1775 0.32μM R02 exp75">
{{:chemogenomics:comparison_plots:exp216_vs_exp75.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Hydroxyurea 100μM R07 exp329  vs  MK-1775 0.32μM R02 exp75">
{{:chemogenomics:comparison_plots:exp329_vs_exp75.png?nolink |}}
</modal>

<modal id="9" size="lg" title="FK-506 30μM R07 exp359  vs  MK-1775 0.32μM R02 exp75">
{{:chemogenomics:comparison_plots:exp359_vs_exp75.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|cyclin A2-CDK2 complex|552.80|CDK2,CCNA2|
|eukaryotic translation initiation factor 4F complex|150.76|EIF4A1,EIF4B,EIF4G1|
|RNA cap binding complex|92.13|EIF4A1,EIF4B,EIF4G1|
|translation factor activity, RNA binding|27.64|GCN1,EIF4A1,EIF4B,EIF4G1|
|transferase complex|5.99|CDK2,SKP2,CCNA2,DCUN1D1,CCNL1,EPC1,FBXO42,FBXL12,PFKP|
</modal>