======= ACSL4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ACSL4
  * **<color #00a2e8>Official Name</color>**: acyl-CoA synthetase long chain family member 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2182|2182]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O60488|O60488]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ACSL4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ACSL4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/300157|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|AMP-binding|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|arachidonate-CoA ligase activity|
|embryonic process involved in female pregnancy|
|decanoate-CoA ligase activity|
|very long-chain fatty acid-CoA ligase activity|
|mitochondria-associated endoplasmic reticulum membrane|
|long-chain fatty acid-CoA ligase activity|
|response to interleukin-15|
|acyl-CoA ligase activity|
|long-chain fatty-acyl-CoA biosynthetic process|
|triglyceride biosynthetic process|
|neutral lipid biosynthetic process|
|acylglycerol biosynthetic process|
|long-chain fatty-acyl-CoA metabolic process|
|dendritic spine development|
|fatty-acyl-CoA biosynthetic process|
|fatty-acyl-CoA metabolic process|
|acyl-CoA biosynthetic process|
|thioester biosynthetic process|
|peroxisomal membrane|
|ribonucleoside bisphosphate biosynthetic process|
|nucleoside bisphosphate biosynthetic process|
|purine nucleoside bisphosphate biosynthetic process|
|fatty acid transport|
|triglyceride metabolic process|
|lipid droplet|
|fatty acid derivative biosynthetic process|
|thioester metabolic process|
|acyl-CoA metabolic process|
|acylglycerol metabolic process|
|neutral lipid metabolic process|
|dendrite development|
|long-chain fatty acid metabolic process|
|nucleoside bisphosphate metabolic process|
|purine nucleoside bisphosphate metabolic process|
|ribonucleoside bisphosphate metabolic process|
|coenzyme biosynthetic process|
|monocarboxylic acid transport|
|purine ribonucleotide biosynthetic process|
|purine nucleotide biosynthetic process|
|ribonucleotide biosynthetic process|
|mitochondrial outer membrane|
|fatty acid derivative metabolic process|
|ribose phosphate biosynthetic process|
|purine-containing compound biosynthetic process|
|female pregnancy|
|sulfur compound biosynthetic process|
|cofactor biosynthetic process|
|multi-multicellular organism process|
|response to nutrient|
|glycerolipid biosynthetic process|
|nucleotide biosynthetic process|
|nucleoside phosphate biosynthetic process|
|coenzyme metabolic process|
|organic acid transport|
|carboxylic acid transport|
|lipid transport|
|purine ribonucleotide metabolic process|
|lipid localization|
|fatty acid metabolic process|
|ribonucleotide metabolic process|
|purine nucleotide metabolic process|
|ribose phosphate metabolic process|
|sulfur compound metabolic process|
|purine-containing compound metabolic process|
|neuronal cell body|
|glycerolipid metabolic process|
|cofactor metabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|organic anion transport|
|response to nutrient levels|
|amide biosynthetic process|
|monocarboxylic acid metabolic process|
|response to extracellular stimulus|
|organophosphate biosynthetic process|
|nucleobase-containing small molecule metabolic process|
|lipid biosynthetic process|
|anion transport|
|carbohydrate derivative biosynthetic process|
|embryo development ending in birth or egg hatching|
|neuron projection development|
|cellular amide metabolic process|
|neuron development|
|multicellular organismal reproductive process|
|multicellular organism reproduction|
|organophosphate metabolic process|
|carboxylic acid metabolic process|
|endoplasmic reticulum membrane|
|cellular lipid metabolic process|
|embryo development|
|multi-organism reproductive process|
|oxoacid metabolic process|
|neuron differentiation|
|organic acid metabolic process|
|carbohydrate derivative metabolic process|
|nucleobase-containing compound biosynthetic process|
|response to cytokine|
|plasma membrane bounded cell projection organization|
|cell projection organization|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|lipid metabolic process|
|organic cyclic compound biosynthetic process|
|ion transport|
|organonitrogen compound biosynthetic process|
|reproductive process|
|reproduction|
|ATP binding|
|generation of neurons|
|neurogenesis|
|cellular nitrogen compound biosynthetic process|
|cell development|
|small molecule metabolic process|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-4.57|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|-4.53|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|-4.13|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-3.33|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|-3.13|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-3.04|
|[[:results:exp422|Wiskostatin 3μM R07 exp422]]|-2.65|
|[[:results:exp457|Bisphenol F 50μM R08 exp457]]|-2.52|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2.37|
|[[:results:exp379|MSC2530818 10μM R07 exp379]]|-2.33|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-2.31|
|[[:results:exp483|FTY720 3μM R08 exp483]]|-2.21|
|[[:results:exp247|UM0130462 0.025 to 0.035μM day4 R05 exp247]]|-2.15|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|-2.11|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|-2.11|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-2.09|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-2.07|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|-2.04|
|[[:results:exp141|Nifurtimox 1μM R03 exp141]]|-1.98|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-1.95|
|[[:results:exp476|Dihydrosphingosine 8μM R08 exp476]]|-1.94|
|[[:results:exp23|Nocodazole 0.02μM R00 exp23]]|-1.94|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-1.91|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|-1.86|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-1.85|
|[[:results:exp406|Thalidomide 20μM R07 exp406]]|-1.84|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|-1.83|
|[[:results:exp484|GSK-J5 1.5μM R08 exp484]]|-1.81|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-1.76|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|-1.75|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-1.72|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|1.71|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|1.75|
|[[:results:exp187|proTAME 5μM R04 exp187]]|1.75|
|[[:results:exp149|SB203580 25μM R03 exp149]]|1.79|
|[[:results:exp410|THZ531 0.11 to 0.125μM on day4 R07 exp410]]|1.82|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|1.85|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|1.88|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|1.91|
|[[:results:exp4|Actinomycin-D 0.01μM R00 exp4]]|1.99|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|2.01|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|2.02|
|[[:results:exp59|UMK57 1μM R01 exp59]]|2.03|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|2.06|
|[[:results:exp120|Dimethyl-Sulfoxide 1pc R03 exp120]]|2.1|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|2.15|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|2.19|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|2.21|
|[[:results:exp413|THZ531 0.11 to 0.175μM on day4 R07 exp413]]|2.24|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|2.26|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|2.4|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|2.43|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|2.61|
|[[:results:exp518|RK-33 8μM R08 exp518]]|2.73|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|2.85|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|2.99|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|3.11|
|[[:results:exp246|UM0011500 10μM R05 exp246]]|3.15|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|3.3|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|3.32|
|[[:results:exp143|Phenformin 20μM R03 exp143]]|3.34|
|[[:results:exp536|Vitamin-D3 40μM R08 exp536]]|3.41|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|3.47|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|4|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.607|
|[[:human genes:m:mmd|MMD]]|0.512|
|[[:human genes:p:pex12|PEX12]]|0.475|
|[[:human genes:l:lpcat3|LPCAT3]]|0.463|
|[[:human genes:a:agpat1|AGPAT1]]|0.461|
|[[:human genes:a:arpc4|ARPC4]]|0.459|
|[[:human genes:l:ldlr|LDLR]]|0.428|
|[[:human genes:m:mdh1|MDH1]]|0.426|
|[[:human genes:v:vkorc1l1|VKORC1L1]]|0.422|
|[[:human genes:p:pex1|PEX1]]|0.413|
|[[:human genes:a:actr2|ACTR2]]|0.412|
|[[:human genes:c:commd2|COMMD2]]|0.412|
|[[:human genes:p:pcyt2|PCYT2]]|0.404|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 4/694
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|2/26|
|bone|0/26|
|breast|0/30|
|central nervous system|0/49|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/11|
|fibroblast|0/1|
|gastric|0/14|
|kidney|0/18|
|liver|0/19|
|lung|0/72|
|lymphocyte|0/16|
|ovary|0/25|
|pancreas|0/22|
|peripheral nervous system|0/15|
|plasma cell|0/12|
|prostate|0/1|
|skin|0/20|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|1/28|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2533
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.68 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ACSL4 Expression in NALM6 Cells: 6.68'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>