======= AGO2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: AGO2
  * **<color #00a2e8>Official Name</color>**: argonaute RISC catalytic component 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=27161|27161]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9UKV8|Q9UKV8]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=AGO2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20AGO2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/606229|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. {ECO:0000255|HAMAP-Rule:MF_03031, ECO:0000269|PubMed:15105377, ECO:0000269|PubMed:15260970, ECO:0000269|PubMed:15284456, ECO:0000269|PubMed:15337849, ECO:0000269|PubMed:15800637, ECO:0000269|PubMed:16081698, ECO:0000269|PubMed:16142218, ECO:0000269|PubMed:16271387, ECO:0000269|PubMed:16289642, ECO:0000269|PubMed:16357216, ECO:0000269|PubMed:16756390, ECO:0000269|PubMed:16936728, ECO:0000269|PubMed:17382880, ECO:0000269|PubMed:17507929, ECO:0000269|PubMed:17524464, ECO:0000269|PubMed:17531811, ECO:0000269|PubMed:17932509, ECO:0000269|PubMed:18048652, ECO:0000269|PubMed:18178619, ECO:0000269|PubMed:18690212, ECO:0000269|PubMed:18771919, ECO:0000269|PubMed:19167051, ECO:0000269|PubMed:23746446}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PAZ|
|DUF1785|
|Piwi|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|endoribonuclease activity, cleaving siRNA-paired mRNA|
|mRNA cap binding|
|regulation of miRNA mediated inhibition of translation|
|endoribonuclease activity, cleaving miRNA-paired mRNA|
|positive regulation of miRNA mediated inhibition of translation|
|mRNA cleavage involved in gene silencing by siRNA|
|siRNA loading onto RISC involved in RNA interference|
|mRNA cleavage involved in gene silencing|
|mRNA cleavage involved in gene silencing by miRNA|
|positive regulation of trophoblast cell migration|
|negative regulation of amyloid precursor protein biosynthetic process|
|miRNA loading onto RISC involved in gene silencing by miRNA|
|RNA secondary structure unwinding|
|small RNA loading onto RISC|
|RISC-loading complex|
|production of siRNA involved in RNA interference|
|siRNA binding|
|regulation of trophoblast cell migration|
|mRNA cap binding complex|
|positive regulation of nuclear-transcribed mRNA poly(A) tail shortening|
|core promoter binding|
|miRNA mediated inhibition of translation|
|negative regulation of translation, ncRNA-mediated|
|regulation of translation, ncRNA-mediated|
|RNA 7-methylguanosine cap binding|
|regulation of amyloid precursor protein biosynthetic process|
|negative regulation of glycoprotein biosynthetic process|
|regulation of nuclear-transcribed mRNA poly(A) tail shortening|
|pre-miRNA processing|
|negative regulation of glycoprotein metabolic process|
|RISC complex|
|RNA interference|
|positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|
|negative regulation of translational initiation|
|regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|
|miRNA metabolic process|
|positive regulation of gene silencing by miRNA|
|positive regulation of posttranscriptional gene silencing|
|mRNA cleavage|
|endoribonuclease activity|
|production of miRNAs involved in gene silencing by miRNA|
|miRNA binding|
|RNA polymerase II complex binding|
|dsRNA processing|
|production of small RNA involved in gene silencing by RNA|
|Wnt signaling pathway, calcium modulating pathway|
|regulation of glycoprotein biosynthetic process|
|polysome|
|regulation of glycoprotein metabolic process|
|gene silencing by miRNA|
|single-stranded RNA binding|
|positive regulation of mRNA catabolic process|
|translation initiation factor activity|
|posttranscriptional gene silencing by RNA|
|posttranscriptional gene silencing|
|double-stranded RNA binding|
|positive regulation of mRNA metabolic process|
|positive regulation of reproductive process|
|regulation of translational initiation|
|RNA phosphodiester bond hydrolysis, endonucleolytic|
|P-body|
|gene silencing by RNA|
|regulation of gene silencing by miRNA|
|post-embryonic development|
|regulation of gene silencing by RNA|
|regulation of posttranscriptional gene silencing|
|regulation of gene silencing|
|negative regulation of translation|
|non-canonical Wnt signaling pathway|
|translational initiation|
|negative regulation of cellular amide metabolic process|
|gene silencing|
|ribonucleoprotein complex|
|RNA phosphodiester bond hydrolysis|
|regulation of reproductive process|
|positive regulation of angiogenesis|
|positive regulation of vasculature development|
|protein C-terminus binding|
|regulation of mRNA catabolic process|
|ribonucleoprotein complex assembly|
|regulation of gene expression, epigenetic|
|ribonucleoprotein complex subunit organization|
|regulation of angiogenesis|
|nucleic acid phosphodiester bond hydrolysis|
|regulation of vasculature development|
|regulation of mRNA metabolic process|
|regulation of translation|
|Wnt signaling pathway|
|cell-cell signaling by wnt|
|positive regulation of cellular catabolic process|
|translation|
|ncRNA processing|
|regulation of cellular amide metabolic process|
|peptide biosynthetic process|
|dendrite|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|positive regulation of catabolic process|
|ribonucleoprotein complex biogenesis|
|ncRNA metabolic process|
|positive regulation of cell migration|
|positive regulation of multi-organism process|
|positive regulation of cell motility|
|posttranscriptional regulation of gene expression|
|amide biosynthetic process|
|peptide metabolic process|
|positive regulation of cellular component movement|
|cell junction|
|positive regulation of locomotion|
|mRNA metabolic process|
|regulation of multi-organism process|
|cellular amide metabolic process|
|regulation of cellular catabolic process|
|cellular protein-containing complex assembly|
|regulation of cell migration|
|RNA processing|
|regulation of cell motility|
|regulation of locomotion|
|regulation of catabolic process|
|regulation of cellular component movement|
|negative regulation of cellular protein metabolic process|
|regulation of anatomical structure morphogenesis|
|negative regulation of protein metabolic process|
|cell-cell signaling|
|positive regulation of transcription by RNA polymerase II|
|positive regulation of developmental process|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|organonitrogen compound biosynthetic process|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|protein-containing complex assembly|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.27|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|-2.18|
|[[:results:exp235|Geldanamycin 0.01μM R05 exp235]]|-2.14|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|-2.06|
|[[:results:exp210|LB-100 2μM R05 exp210]]|-2.06|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.04|
|[[:results:exp245|UM0011500 5μM R05 exp245]]|-2|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|-1.95|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|-1.95|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-1.95|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|-1.87|
|[[:results:exp534|Trientine 500μM R08 exp534]]|-1.87|
|[[:results:exp246|UM0011500 10μM R05 exp246]]|-1.81|
|[[:results:exp242|Radicicol 0.16μM R05 exp242]]|-1.76|
|[[:results:exp272|CHIR-124 0.04μM R06 exp272]]|-1.73|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|-1.73|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|-1.73|
|[[:results:exp260|ABT-702 0.1μM R06 exp260]]|-1.71|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|1.83|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|1.84|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|1.91|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:s:scaf8|SCAF8]]|0.508|
|[[:human genes:l:lmo2|LMO2]]|0.456|
|[[:human genes:a:ankrd52|ANKRD52]]|0.456|
|[[:human genes:i:id3|ID3]]|0.45|
|[[:human genes:l:ldb1|LDB1]]|0.439|
|[[:human genes:d:dohh|DOHH]]|0.428|
|[[:human genes:r:rnmt|RNMT]]|0.424|
|[[:human genes:b:bap1|BAP1]]|0.42|
|[[:human genes:z:znf410|ZNF410]]|0.415|
|[[:human genes:c:cbx3|CBX3]]|0.414|
|[[:human genes:c:ccdc101|CCDC101]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5278
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.11 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='AGO2 Expression in NALM6 Cells: 7.11'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>