======= AP2M1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: AP2M1
  * **<color #00a2e8>Official Name</color>**: adaptor related protein complex 2 subunit mu 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1173|1173]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96CW1|Q96CW1]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=AP2M1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20AP2M1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601024|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X- X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane- associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity). {ECO:0000250, ECO:0000269|PubMed:12694563, ECO:0000269|PubMed:12952941, ECO:0000269|PubMed:14745134, ECO:0000269|PubMed:14985334, ECO:0000269|PubMed:15473838, ECO:0000269|PubMed:16581796, ECO:0000269|PubMed:19033387}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Adap comp sub|
|Clat adaptor s|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|regulation of vesicle size|
|signal sequence binding|
|low-density lipoprotein particle receptor catabolic process|
|clathrin adaptor activity|
|low-density lipoprotein receptor particle metabolic process|
|endolysosome membrane|
|AP-2 adaptor complex|
|low-density lipoprotein particle receptor binding|
|negative regulation of protein localization to plasma membrane|
|negative regulation of protein localization to cell periphery|
|low-density lipoprotein particle clearance|
|clathrin-coated endocytic vesicle|
|clathrin-dependent endocytosis|
|receptor catabolic process|
|regulation of defense response to virus by virus|
|negative regulation of protein localization to membrane|
|clathrin-coated endocytic vesicle membrane|
|plasma lipoprotein particle clearance|
|receptor internalization|
|endocytic vesicle membrane|
|regulation of plasma lipoprotein particle levels|
|ephrin receptor signaling pathway|
|regulation of protein localization to plasma membrane|
|vesicle budding from membrane|
|Wnt signaling pathway, planar cell polarity pathway|
|antigen processing and presentation of exogenous peptide antigen via MHC class II|
|antigen processing and presentation of peptide antigen via MHC class II|
|antigen processing and presentation of peptide or polysaccharide antigen via MHC class II|
|receptor metabolic process|
|regulation of establishment of planar polarity|
|negative regulation of cellular protein localization|
|regulation of protein localization to cell periphery|
|ion channel binding|
|non-canonical Wnt signaling pathway|
|lipid binding|
|antigen processing and presentation of exogenous peptide antigen|
|regulation of morphogenesis of an epithelium|
|antigen processing and presentation of exogenous antigen|
|regulation of protein localization to membrane|
|antigen processing and presentation of peptide antigen|
|antigen processing and presentation|
|receptor-mediated endocytosis|
|regulation of animal organ morphogenesis|
|lysosomal membrane|
|vesicle organization|
|cell-cell signaling by wnt|
|Wnt signaling pathway|
|regulation of cellular component size|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|regulation of anatomical structure size|
|regulation of cellular protein localization|
|endocytosis|
|cellular protein catabolic process|
|import into cell|
|protein catabolic process|
|viral process|
|enzyme linked receptor protein signaling pathway|
|symbiotic process|
|interspecies interaction between organisms|
|cellular protein-containing complex assembly|
|membrane organization|
|cellular macromolecule catabolic process|
|regulation of cellular localization|
|intracellular protein transport|
|regulation of protein localization|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|regulation of anatomical structure morphogenesis|
|cell-cell signaling|
|protein transport|
|intracellular transport|
|peptide transport|
|protein-containing complex assembly|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|organic substance catabolic process|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|protein-containing complex subunit organization|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-1.8|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-1.75|
|[[:results:exp50|Nicotinamide 2000μM R01 exp50]]|1.88|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|1.96|
|[[:results:exp362|GSK-J4 1μM R07 exp362]]|1.99|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.491|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 232/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|11/28|
|blood|16/28|
|bone|6/26|
|breast|16/33|
|central nervous system|6/56|
|cervix|0/4|
|colorectal|5/17|
|esophagus|2/13|
|fibroblast|0/1|
|gastric|5/16|
|kidney|8/21|
|liver|8/20|
|lung|25/75|
|lymphocyte|7/16|
|ovary|7/26|
|pancreas|12/24|
|peripheral nervous system|5/16|
|plasma cell|6/15|
|prostate|0/1|
|skin|7/24|
|soft tissue|1/9|
|thyroid|0/2|
|upper aerodigestive|2/22|
|urinary tract|9/29|
|uterus|2/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1452
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.52 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='AP2M1 Expression in NALM6 Cells: 7.52'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>