======= APLF =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: APLF
  * **<color #00a2e8>Official Name</color>**: aprataxin and PNKP like factor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=200558|200558]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8IW19|Q8IW19]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=APLF&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20APLF|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/611035|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. {ECO:0000269|PubMed:17353262, ECO:0000269|PubMed:17396150}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|zf-CCHH|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of DNA ligation|
|regulation of DNA ligation|
|single strand break repair|
|endodeoxyribonuclease activity|
|class I DNA-(apurinic or apyrimidinic site) endonuclease activity|
|DNA-(apurinic or apyrimidinic site) endonuclease activity|
|3-5 exonuclease activity|
|regulation of isotype switching|
|regulation of immunoglobulin mediated immune response|
|regulation of B cell mediated immunity|
|site of double-strand break|
|nucleotide binding|
|regulation of immunoglobulin production|
|regulation of DNA recombination|
|regulation of production of molecular mediator of immune response|
|regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains|
|regulation of lymphocyte mediated immunity|
|regulation of adaptive immune response|
|double-strand break repair|
|positive regulation of DNA metabolic process|
|regulation of leukocyte mediated immunity|
|regulation of B cell activation|
|nucleic acid phosphodiester bond hydrolysis|
|regulation of DNA metabolic process|
|regulation of immune effector process|
|DNA repair|
|regulation of lymphocyte activation|
|regulation of leukocyte activation|
|regulation of cell activation|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|regulation of immune response|
|regulation of immune system process|
|cellular response to stress|
|positive regulation of nucleobase-containing compound metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp389|PF-06409577 20μM R07 exp389]]|2.07|
|[[:results:exp423|Zebularine 20μM R07 exp423]]|2.09|
|[[:results:exp316|Geldanamycin 0.015 to 0.025μM on day4 R07 exp316]]|2.2|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 15799
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 2.37 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='APLF Expression in NALM6 Cells: 2.37'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>