======= ASCL1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ASCL1
  * **<color #00a2e8>Official Name</color>**: achaete-scute family bHLH transcription factor 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=429|429]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P50553|P50553]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ASCL1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ASCL1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/100790|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. {ECO:0000250|UniProtKB:Q02067}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|HLH|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|lung neuroendocrine cell differentiation|
|noradrenergic neuron fate commitment|
|neuroblast fate determination|
|carotid body glomus cell differentiation|
|regulation of timing of subpallium neuron differentiation|
|vestibular nucleus development|
|neuroblast fate commitment|
|stomach neuroendocrine cell differentiation|
|stomach development|
|adrenal chromaffin cell differentiation|
|response to magnetism|
|subpallium neuron fate commitment|
|medulla oblongata development|
|musculoskeletal movement, spinal reflex action|
|cellular response to magnetism|
|spinal cord oligodendrocyte cell fate specification|
|spinal cord oligodendrocyte cell differentiation|
|noradrenergic neuron development|
|stem cell fate determination|
|olfactory pit development|
|neuroblast differentiation|
|regulation of timing of neuron differentiation|
|glial cell fate specification|
|oligodendrocyte cell fate specification|
|oligodendrocyte cell fate commitment|
|neuroendocrine cell differentiation|
|commitment of neuronal cell to specific neuron type in forebrain|
|noradrenergic neuron differentiation|
|sympathetic ganglion development|
|stem cell fate commitment|
|lung secretory cell differentiation|
|pons development|
|forebrain neuron fate commitment|
|response to folic acid|
|cerebral cortex GABAergic interneuron differentiation|
|regulation of timing of cell differentiation|
|spinal cord association neuron differentiation|
|regulation of development, heterochronic|
|glial cell fate commitment|
|GABAergic neuron differentiation|
|cell fate commitment involved in pattern specification|
|nose development|
|ventral spinal cord interneuron fate commitment|
|ganglion development|
|ventral spinal cord interneuron differentiation|
|reflex|
|lung epithelial cell differentiation|
|sympathetic nervous system development|
|lung cell differentiation|
|spinal cord dorsal/ventral patterning|
|response to lithium ion|
|dorsal spinal cord development|
|subpallium development|
|cerebral cortex neuron differentiation|
|spinal cord patterning|
|adrenal gland development|
|bHLH transcription factor binding|
|lung epithelium development|
|neuroblast proliferation|
|neuron fate specification|
|glandular epithelial cell differentiation|
|cell fate determination|
|oligodendrocyte development|
|multicellular organismal movement|
|musculoskeletal movement|
|neuroepithelial cell differentiation|
|autonomic nervous system development|
|response to epidermal growth factor|
|ventral spinal cord development|
|E-box binding|
|forebrain neuron differentiation|
|positive regulation of Notch signaling pathway|
|positive regulation of neural precursor cell proliferation|
|stem cell proliferation|
|positive regulation of neuron apoptotic process|
|cell differentiation in spinal cord|
|neural nucleus development|
|neuron fate commitment|
|forebrain generation of neurons|
|oligodendrocyte differentiation|
|RNA polymerase II transcription factor complex|
|neural precursor cell proliferation|
|central nervous system neuron development|
|dorsal/ventral pattern formation|
|response to vitamin|
|cell fate specification|
|regulation of neural precursor cell proliferation|
|positive regulation of neuron death|
|columnar/cuboidal epithelial cell differentiation|
|regulation of Notch signaling pathway|
|spinal cord development|
|glial cell development|
|response to retinoic acid|
|metencephalon development|
|cerebral cortex development|
|neuron migration|
|endocrine system development|
|Notch signaling pathway|
|digestive tract development|
|regulation of epithelial cell differentiation|
|digestive system development|
|hindbrain development|
|stem cell differentiation|
|cell maturation|
|lung development|
|glial cell differentiation|
|respiratory tube development|
|pallium development|
|central nervous system neuron differentiation|
|respiratory system development|
|regulation of neuron apoptotic process|
|gliogenesis|
|response to nutrient|
|negative regulation of neuron differentiation|
|developmental maturation|
|DNA-binding transcription repressor activity, RNA polymerase II-specific|
|cell fate commitment|
|telencephalon development|
|negative regulation of neurogenesis|
|regulation of neuron death|
|negative regulation of nervous system development|
|RNA polymerase II regulatory region sequence-specific DNA binding|
|cellular response to environmental stimulus|
|cellular response to abiotic stimulus|
|regionalization|
|transcription factor binding|
|negative regulation of cell development|
|response to acid chemical|
|positive regulation of neuron differentiation|
|response to metal ion|
|neuronal cell body|
|positive regulation of cell cycle|
|forebrain development|
|chromatin binding|
|gland development|
|pattern specification process|
|positive regulation of neurogenesis|
|response to nutrient levels|
|RNA polymerase II proximal promoter sequence-specific DNA binding|
|heart development|
|response to growth factor|
|response to extracellular stimulus|
|response to inorganic substance|
|positive regulation of nervous system development|
|positive regulation of cell development|
|sensory organ development|
|cell population proliferation|
|regulation of mitotic cell cycle|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|regulation of neuron differentiation|
|DNA-binding transcription factor activity|
|epithelial cell differentiation|
|positive regulation of cell death|
|negative regulation of cell differentiation|
|brain development|
|head development|
|neuron development|
|regulation of neurogenesis|
|tube development|
|response to lipid|
|negative regulation of transcription by RNA polymerase II|
|circulatory system development|
|protein homodimerization activity|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|positive regulation of cell population proliferation|
|response to organic cyclic compound|
|regulation of nervous system development|
|regulation of cell development|
|negative regulation of developmental process|
|cell migration|
|positive regulation of cell differentiation|
|central nervous system development|
|negative regulation of cell death|
|response to organonitrogen compound|
|neuron differentiation|
|response to drug|
|cell motility|
|localization of cell|
|response to nitrogen compound|
|epithelium development|
|response to abiotic stimulus|
|regulation of cell cycle|
|negative regulation of transcription, DNA-templated|
|negative regulation of multicellular organismal process|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|locomotion|
|negative regulation of RNA metabolic process|
|positive regulation of developmental process|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|regulation of apoptotic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|movement of cell or subcellular component|
|response to oxygen-containing compound|
|DNA-binding transcription factor activity, RNA polymerase II-specific|
|regulation of programmed cell death|
|regulation of cell population proliferation|
|neurogenesis|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|cell development|
|positive regulation of signal transduction|
|regulation of cell death|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|tissue development|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp287|HMS-I2 5μM R06 exp287]]|1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 3/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|1/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 16018
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: -0.41 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ASCL1 Expression in NALM6 Cells: -0.41'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>