======= ATG3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ATG3
  * **<color #00a2e8>Official Name</color>**: autophagy related 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=64422|64422]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9NT62|Q9NT62]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ATG3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ATG3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/609606|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. {ECO:0000269|PubMed:11825910, ECO:0000269|PubMed:12207896, ECO:0000269|PubMed:12890687, ECO:0000269|PubMed:16704426, ECO:0000269|PubMed:20723759}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Autophagy Cterm|
|Autophagy act C|
|Autophagy N|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|cytoplasmic ubiquitin ligase complex|
|Atg12 transferase activity|
|Atg8 ligase activity|
|ubiquitin-like protein transferase activity|
|mitochondrial fragmentation involved in apoptotic process|
|negative regulation of phagocytosis|
|mitochondrion disassembly|
|autophagy of mitochondrion|
|apoptotic mitochondrial changes|
|autophagosome assembly|
|organelle disassembly|
|autophagosome organization|
|regulation of cilium assembly|
|regulation of phagocytosis|
|vacuole organization|
|protein targeting to membrane|
|macroautophagy|
|regulation of plasma membrane bounded cell projection assembly|
|regulation of cell projection assembly|
|regulation of organelle assembly|
|autophagy|
|process utilizing autophagic mechanism|
|establishment of protein localization to membrane|
|enzyme binding|
|protein targeting|
|cellular component disassembly|
|mitochondrion organization|
|protein localization to membrane|
|negative regulation of transport|
|regulation of vesicle-mediated transport|
|protein ubiquitination|
|regulation of plasma membrane bounded cell projection organization|
|regulation of cell projection organization|
|organelle assembly|
|protein modification by small protein conjugation|
|apoptotic process|
|regulation of cellular component biogenesis|
|protein modification by small protein conjugation or removal|
|intracellular protein transport|
|programmed cell death|
|cell death|
|regulation of organelle organization|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|-2.02|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|-1.81|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|1.8|
|[[:results:exp51|Nifuroxazide 1μM R01 exp51]]|1.81|
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.82|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|1.87|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.553|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11214
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.96 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ATG3 Expression in NALM6 Cells: 5.96'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>