======= BRD4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: BRD4
  * **<color #00a2e8>Official Name</color>**: bromodomain containing 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=23476|23476]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O60885|O60885]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=BRD4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20BRD4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608749|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. {ECO:0000269|PubMed:22509028}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Bromodomain|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of histone H3-K36 trimethylation|
|P-TEFb complex binding|
|regulation of phosphorylation of RNA polymerase II C-terminal domain|
|transcription regulator recruiting activity|
|positive regulation of histone H3-K36 methylation|
|regulation of histone H3-K36 trimethylation|
|RNA polymerase II C-terminal domain binding|
|regulation of histone H3-K36 methylation|
|RNA polymerase II CTD heptapeptide repeat kinase activity|
|positive regulation of transcription elongation from RNA polymerase II promoter|
|lysine-acetylated histone binding|
|positive regulation of G2/M transition of mitotic cell cycle|
|regulation of transcription involved in G1/S transition of mitotic cell cycle|
|condensed nuclear chromosome|
|positive regulation of cell cycle G2/M phase transition|
|regulation of transcription elongation from RNA polymerase II promoter|
|positive regulation of DNA-templated transcription, elongation|
|positive regulation of histone methylation|
|regulation of DNA-templated transcription, elongation|
|regulation of histone methylation|
|p53 binding|
|positive regulation of mitotic cell cycle phase transition|
|positive regulation of histone modification|
|positive regulation of cell cycle phase transition|
|positive regulation of chromatin organization|
|G1/S transition of mitotic cell cycle|
|cell cycle G1/S phase transition|
|regulation of histone modification|
|positive regulation of mitotic cell cycle|
|positive regulation of chromosome organization|
|positive regulation of I-kappaB kinase/NF-kappaB signaling|
|regulation of chromatin organization|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of cell cycle G2/M phase transition|
|regulation of I-kappaB kinase/NF-kappaB signaling|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|positive regulation of cell cycle process|
|regulation of inflammatory response|
|enzyme binding|
|regulation of chromosome organization|
|positive regulation of cell cycle|
|chromatin binding|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|mitotic cell cycle process|
|positive regulation of organelle organization|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|chromatin organization|
|viral process|
|regulation of cell cycle process|
|regulation of defense response|
|cellular response to DNA damage stimulus|
|symbiotic process|
|interspecies interaction between organisms|
|protein phosphorylation|
|cell cycle process|
|positive regulation of intracellular signal transduction|
|chromosome organization|
|regulation of response to external stimulus|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|positive regulation of protein modification process|
|phosphorylation|
|regulation of organelle organization|
|cell cycle|
|regulation of protein phosphorylation|
|regulation of response to stress|
|positive regulation of transcription, DNA-templated|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|positive regulation of signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|positive regulation of RNA metabolic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp70|INK128 0.2μM R02 exp70]]|-1.81|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|1.72|
|[[:results:exp476|Dihydrosphingosine 8μM R08 exp476]]|1.72|
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|1.76|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|1.79|
|[[:results:exp224|CB-839 10μM R05 exp224]]|1.81|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|1.82|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|1.86|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|2|
|[[:results:exp318|ABT-702 5μM R07 exp318]]|2.04|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|2.11|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|2.23|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.452|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.43|
|[[:human genes:p:prpf8|PRPF8]]|0.423|
|[[:human genes:s:sfpq|SFPQ]]|0.415|
|[[:human genes:p:polr2j3|POLR2J3]]|0.408|
|[[:human genes:k:kif3c|KIF3C]]|0.405|
|[[:human genes:p:prim1|PRIM1]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 519/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|24/28|
|blood|21/28|
|bone|18/25|
|breast|20/33|
|central nervous system|38/56|
|cervix|2/4|
|colorectal|12/17|
|esophagus|10/13|
|fibroblast|1/1|
|gastric|11/15|
|kidney|11/21|
|liver|15/20|
|lung|53/75|
|lymphocyte|12/14|
|ovary|19/26|
|pancreas|17/24|
|peripheral nervous system|10/16|
|plasma cell|14/15|
|prostate|1/1|
|skin|20/24|
|soft tissue|3/7|
|thyroid|2/2|
|upper aerodigestive|14/22|
|urinary tract|21/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 744
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.07 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='BRD4 Expression in NALM6 Cells: 7.07'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>