======= BUB3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: BUB3
  * **<color #00a2e8>Official Name</color>**: BUB3 mitotic checkpoint protein
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9184|9184]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O43684|O43684]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=BUB3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20BUB3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603719|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:18199686}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WD40|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|bub1-bub3 complex|
|mitotic checkpoint complex|
|protein localization to kinetochore|
|protein localization to chromosome, centromeric region|
|attachment of spindle microtubules to kinetochore|
|mitotic spindle assembly checkpoint|
|spindle checkpoint|
|spindle assembly checkpoint|
|mitotic spindle checkpoint|
|negative regulation of mitotic metaphase/anaphase transition|
|negative regulation of metaphase/anaphase transition of cell cycle|
|negative regulation of mitotic sister chromatid separation|
|negative regulation of chromosome separation|
|negative regulation of mitotic sister chromatid segregation|
|negative regulation of sister chromatid segregation|
|negative regulation of chromosome segregation|
|negative regulation of mitotic nuclear division|
|negative regulation of nuclear division|
|regulation of mitotic metaphase/anaphase transition|
|regulation of metaphase/anaphase transition of cell cycle|
|regulation of mitotic sister chromatid separation|
|protein localization to chromosome|
|regulation of chromosome separation|
|regulation of mitotic sister chromatid segregation|
|ubiquitin binding|
|regulation of sister chromatid segregation|
|anaphase-promoting complex-dependent catabolic process|
|kinetochore|
|condensed chromosome kinetochore|
|regulation of chromosome segregation|
|mitotic sister chromatid segregation|
|negative regulation of chromosome organization|
|sister chromatid segregation|
|mitotic nuclear division|
|mitotic cell cycle checkpoint|
|regulation of mitotic nuclear division|
|regulation of nuclear division|
|cell cycle checkpoint|
|negative regulation of mitotic cell cycle phase transition|
|nuclear chromosome segregation|
|meiotic cell cycle|
|negative regulation of cell cycle phase transition|
|chromosome segregation|
|nuclear division|
|negative regulation of mitotic cell cycle|
|organelle fission|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|negative regulation of cell cycle process|
|proteasomal protein catabolic process|
|regulation of chromosome organization|
|negative regulation of organelle organization|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|cell division|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|modification-dependent macromolecule catabolic process|
|negative regulation of cell cycle|
|proteolysis involved in cellular protein catabolic process|
|mitotic cell cycle process|
|cellular protein catabolic process|
|regulation of mitotic cell cycle|
|protein catabolic process|
|mitotic cell cycle|
|negative regulation of cellular component organization|
|protein localization to organelle|
|regulation of cell cycle process|
|cellular macromolecule catabolic process|
|cell cycle process|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|chromosome organization|
|regulation of cell cycle|
|proteolysis|
|regulation of organelle organization|
|cell cycle|
|reproductive process|
|reproduction|
|cellular protein localization|
|cellular macromolecule localization|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|-2.26|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.21|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|-2.12|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-2.09|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-1.92|
|[[:results:exp481|Ethambutol 25μM R08 exp481]]|-1.85|
|[[:results:exp471|Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471]]|-1.84|
|[[:results:exp333|All-trans-Retinoic-Acid 8μM R07 exp333]]|-1.81|
|[[:results:exp293|Myriocin 25μM R06 exp293]]|-1.81|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-1.79|
|[[:results:exp477|DKK1 89ng/ml R08 exp477]]|-1.72|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|-1.71|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|-1.71|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|1.74|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|1.76|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|1.88|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|1.92|
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|3.04|
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|3.45|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|3.56|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|3.95|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|4.04|
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|4.17|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|4.5|
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|4.81|
|[[:results:exp17|DABN 20μM R00 exp17]]|4.85|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|5.54|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|5.75|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|5.75|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|5.78|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|5.89|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|6|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|6.01|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|6.86|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|6.9|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|6.9|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mad1l1|MAD1L1]]|0.801|
|[[:human genes:m:mad2l1|MAD2L1]]|0.799|
|[[:human genes:r:rbm14|RBM14]]|0.763|
|[[:human genes:k:kntc1|KNTC1]]|0.72|
|[[:human genes:z:zw10|ZW10]]|0.707|
|[[:human genes:z:zwilch|ZWILCH]]|0.684|
|[[:human genes:t:trip13|TRIP13]]|0.678|
|[[:human genes:b:bub1b|BUB1B]]|0.663|
|[[:human genes:m:mad2l1bp|MAD2L1BP]]|0.621|
|[[:human genes:m:med16|MED16]]|0.594|
|[[:human genes:f:fnta|FNTA]]|0.591|
|[[:human genes:p:pelo|PELO]]|0.579|
|[[:human genes:f:fntb|FNTB]]|0.56|
|[[:human genes:c:c14orf80|C14orf80]]|0.541|
|[[:human genes:d:dbr1|DBR1]]|0.517|
|[[:human genes:r:rnmt|RNMT]]|0.509|
|[[:human genes:d:dpagt1|DPAGT1]]|0.508|
|[[:human genes:t:thg1l|THG1L]]|0.502|
|[[:human genes:t:tubd1|TUBD1]]|0.5|
|[[:human genes:d:ddx20|DDX20]]|0.485|
|[[:human genes:c:c16orf59|C16orf59]]|0.476|
|[[:human genes:a:alg1|ALG1]]|0.471|
|[[:human genes:g:gnb1l|GNB1L]]|0.464|
|[[:human genes:r:rtcb|RTCB]]|0.464|
|[[:human genes:s:srp72|SRP72]]|0.45|
|[[:human genes:t:timm22|TIMM22]]|0.448|
|[[:human genes:t:tti2|TTI2]]|0.447|
|[[:human genes:s:srp14|SRP14]]|0.445|
|[[:human genes:s:snapc5|SNAPC5]]|0.444|
|[[:human genes:p:ppp4c|PPP4C]]|0.443|
|[[:human genes:t:tsen54|TSEN54]]|0.442|
|[[:human genes:g:gtf3c4|GTF3C4]]|0.441|
|[[:human genes:o:oraov1|ORAOV1]]|0.44|
|[[:human genes:u:uap1|UAP1]]|0.436|
|[[:human genes:c:cdipt|CDIPT]]|0.43|
|[[:human genes:t:tube1|TUBE1]]|0.429|
|[[:human genes:t:tgs1|TGS1]]|0.427|
|[[:human genes:g:gemin8|GEMIN8]]|0.426|
|[[:human genes:h:hspd1|HSPD1]]|0.426|
|[[:human genes:t:tmem41b|TMEM41B]]|0.425|
|[[:human genes:a:atxn10|ATXN10]]|0.421|
|[[:human genes:a:adsl|ADSL]]|0.42|
|[[:human genes:r:rttn|RTTN]]|0.418|
|[[:human genes:o:osgep|OSGEP]]|0.417|
|[[:human genes:t:tti1|TTI1]]|0.415|
|[[:human genes:g:gtf3c2|GTF3C2]]|0.415|
|[[:human genes:n:nsmce1|NSMCE1]]|0.414|
|[[:human genes:p:pam16|PAM16]]|0.413|
|[[:human genes:w:wdr1|WDR1]]|0.413|
|[[:human genes:p:polr3h|POLR3H]]|0.412|
|[[:human genes:g:gemin6|GEMIN6]]|0.411|
|[[:human genes:r:rpp21|RPP21]]|0.411|
|[[:human genes:p:pmm2|PMM2]]|0.41|
|[[:human genes:d:dctn6|DCTN6]]|0.409|
|[[:human genes:t:tbca|TBCA]]|0.406|
|[[:human genes:r:rabggtb|RABGGTB]]|0.403|
|[[:human genes:c:ciao1|CIAO1]]|0.403|
|[[:human genes:d:dhps|DHPS]]|0.402|
|[[:human genes:c:ccdc115|CCDC115]]|0.401|
|[[:human genes:t:trmt5|TRMT5]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 735/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|26/26|
|breast|31/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 148
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.01 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='BUB3 Expression in NALM6 Cells: 8.01'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>