======= CAST =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: CAST
  * **<color #00a2e8>Official Name</color>**: calpastatin
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=831|831]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P20810|P20810]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=CAST&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20CAST|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/114090|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]. COMPLETENESS: complete on the 3' end. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Calpain inhib|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|calcium-dependent cysteine-type endopeptidase inhibitor activity|
|negative regulation of type B pancreatic cell apoptotic process|
|presynaptic active zone organization|
|regulation of type B pancreatic cell apoptotic process|
|inhibition of cysteine-type endopeptidase activity|
|zymogen inhibition|
|negative regulation of protein processing|
|negative regulation of protein maturation|
|endopeptidase inhibitor activity|
|negative regulation of epithelial cell apoptotic process|
|regulation of protein processing|
|regulation of protein maturation|
|regulation of epithelial cell apoptotic process|
|negative regulation of cysteine-type endopeptidase activity|
|protease binding|
|regulation of cysteine-type endopeptidase activity|
|negative regulation of endopeptidase activity|
|negative regulation of peptidase activity|
|synapse organization|
|cadherin binding|
|negative regulation of proteolysis|
|regulation of endopeptidase activity|
|regulation of peptidase activity|
|negative regulation of hydrolase activity|
|regulation of proteolysis|
|negative regulation of catalytic activity|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|negative regulation of cell death|
|endoplasmic reticulum|
|negative regulation of cellular protein metabolic process|
|negative regulation of protein metabolic process|
|negative regulation of molecular function|
|regulation of hydrolase activity|
|RNA binding|
|regulation of apoptotic process|
|regulation of programmed cell death|
|regulation of cell death|
|negative regulation of gene expression|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|-1.85|
|[[:results:exp131|L-74142 5μM R03 exp131]]|-1.85|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|1.75|
|[[:results:exp11|CCCP 1μM R00 exp11]]|1.82|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|1.82|
|[[:results:exp40|2-Methoxyestradiol 0.2μM R01 exp40]]|2|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|2.06|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:v:vasn|VASN]]|0.406|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 14997
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.45 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='CAST Expression in NALM6 Cells: 5.45'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>