======= CDK8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: CDK8
  * **<color #00a2e8>Official Name</color>**: cyclin dependent kinase 8
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1024|1024]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P49336|P49336]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=CDK8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20CDK8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603184|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. {ECO:0000269|PubMed:10993082, ECO:0000269|PubMed:15546612}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase Tyr|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|RNA polymerase II CTD heptapeptide repeat kinase activity|
|cyclin-dependent protein serine/threonine kinase activity|
|mediator complex|
|transcription initiation from RNA polymerase II promoter|
|DNA-templated transcription, initiation|
|protein kinase activity|
|transcription by RNA polymerase II|
|protein-containing complex|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|nucleolus|
|protein phosphorylation|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|regulation of cell cycle|
|positive regulation of transcription by RNA polymerase II|
|phosphorylation|
|organic cyclic compound biosynthetic process|
|ATP binding|
|positive regulation of transcription, DNA-templated|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|-2.29|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|-2.29|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|-1.93|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|-1.91|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|-1.77|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.7|
|[[:results:exp72|LB-100 4.1μM R02 exp72]]|1.72|
|[[:results:exp413|THZ531 0.11 to 0.175μM on day4 R07 exp413]]|1.72|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|1.75|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|1.76|
|[[:results:exp70|INK128 0.2μM R02 exp70]]|1.76|
|[[:results:exp417|Tubastatin-A 2.5μM R07 exp417]]|1.78|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|1.84|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|1.85|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|1.85|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|1.87|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|1.88|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|1.92|
|[[:results:exp333|All-trans-Retinoic-Acid 8μM R07 exp333]]|1.95|
|[[:results:exp50|Nicotinamide 2000μM R01 exp50]]|1.97|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|2.01|
|[[:results:exp376|Losmapimod 1μM R07 exp376]]|2.05|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|2.06|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|2.06|
|[[:results:exp418|Tunicamycin 0.04μM R07 exp418]]|2.09|
|[[:results:exp362|GSK-J4 1μM R07 exp362]]|2.28|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|2.32|
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|2.38|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|2.42|
|[[:results:exp83|Trametinib 10μM R02 exp83]]|2.43|
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|2.6|
|[[:results:exp504|MK2206 4μM R08 exp504]]|2.81|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|3.01|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|3.54|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|3.81|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|4|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:ctnnbl1|CTNNBL1]]|0.496|
|[[:human genes:m:memo1|MEMO1]]|0.491|
|[[:human genes:c:cdc73|CDC73]]|0.473|
|[[:human genes:f:fam122a|FAM122A]]|0.472|
|[[:human genes:g:glyr1|GLYR1]]|0.464|
|[[:human genes:p:pcgf1|PCGF1]]|0.464|
|[[:human genes:c:cnot2|CNOT2]]|0.461|
|[[:human genes:e:eif4b|EIF4B]]|0.455|
|[[:human genes:u:ube2h|UBE2H]]|0.455|
|[[:human genes:y:ypel5|YPEL5]]|0.454|
|[[:human genes:u:ube2e1|UBE2E1]]|0.45|
|[[:human genes:z:znf148|ZNF148]]|0.448|
|[[:human genes:t:tsc2|TSC2]]|0.438|
|[[:human genes:r:rps3a|RPS3A]]|0.436|
|[[:human genes:c:ctr9|CTR9]]|0.429|
|[[:human genes:r:ring1|RING1]]|0.428|
|[[:human genes:p:ppie|PPIE]]|0.421|
|[[:human genes:t:tbc1d7|TBC1D7]]|0.418|
|[[:human genes:k:kdm6a|KDM6A]]|0.415|
|[[:human genes:a:anp32a|ANP32A]]|0.413|
|[[:human genes:r:rps3|RPS3]]|0.412|
|[[:human genes:d:ddx11|DDX11]]|0.41|
|[[:human genes:c:cnot1|CNOT1]]|0.406|
|[[:human genes:f:fkbp1a|FKBP1A]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 7/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|2/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|2/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|1/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 18551
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.73 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='CDK8 Expression in NALM6 Cells: 4.73'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>