======= DFFA =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DFFA
  * **<color #00a2e8>Official Name</color>**: DNA fragmentation factor subunit alpha
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1676|1676]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O00273|O00273]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DFFA&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DFFA|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601882|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Inhibitor of the caspase-activated DNase (DFF40).

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|CIDE-N|
|DFF-C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|deoxyribonuclease inhibitor activity|
|negative regulation of deoxyribonuclease activity|
|negative regulation of apoptotic DNA fragmentation|
|negative regulation of DNA catabolic process|
|thymocyte apoptotic process|
|negative regulation of nuclease activity|
|regulation of deoxyribonuclease activity|
|regulation of apoptotic DNA fragmentation|
|regulation of DNA catabolic process|
|T cell apoptotic process|
|apoptotic DNA fragmentation|
|lymphocyte apoptotic process|
|regulation of nuclease activity|
|protein folding chaperone|
|leukocyte apoptotic process|
|DNA catabolic process, endonucleolytic|
|negative regulation of execution phase of apoptosis|
|apoptotic nuclear changes|
|cellular component disassembly involved in execution phase of apoptosis|
|DNA catabolic process|
|regulation of execution phase of apoptosis|
|execution phase of apoptosis|
|chaperone-mediated protein folding|
|lipid droplet|
|negative regulation of DNA metabolic process|
|protein folding|
|nuclear chromatin|
|protein domain specific binding|
|negative regulation of cellular catabolic process|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of catabolic process|
|regulation of DNA metabolic process|
|nucleobase-containing compound catabolic process|
|cellular component disassembly|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|negative regulation of hydrolase activity|
|organic cyclic compound catabolic process|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|positive regulation of cell death|
|negative regulation of cellular component organization|
|DNA metabolic process|
|negative regulation of catalytic activity|
|regulation of cellular catabolic process|
|negative regulation of apoptotic process|
|cellular macromolecule catabolic process|
|negative regulation of programmed cell death|
|apoptotic process|
|negative regulation of cell death|
|regulation of catabolic process|
|macromolecule catabolic process|
|programmed cell death|
|cell death|
|negative regulation of molecular function|
|regulation of hydrolase activity|
|negative regulation of nucleobase-containing compound metabolic process|
|regulation of apoptotic process|
|regulation of programmed cell death|
|regulation of cell death|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp409|THZ531 0.11μM R07 exp409]]|1.8|
|[[:results:exp319|ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319]]|1.88|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|1.96|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|1.98|
|[[:results:exp360|Genistein 15μM R07 exp360]]|2.08|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5548
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.65 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DFFA Expression in NALM6 Cells: 6.65'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>