======= DOT1L =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DOT1L
  * **<color #00a2e8>Official Name</color>**: DOT1 like histone lysine methyltransferase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=84444|84444]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8TEK3|Q8TEK3]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DOT1L&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DOT1L|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607375|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DOT1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|histone methyltransferase activity (H3-K79 specific)|
|histone H3-K79 methylation|
|chromatin silencing at telomere|
|histone methyltransferase activity|
|histone-lysine N-methyltransferase activity|
|chromosome, telomeric region|
|regulation of transcription regulatory region DNA binding|
|chromatin organization involved in negative regulation of transcription|
|chromatin silencing|
|chromatin organization involved in regulation of transcription|
|histone lysine methylation|
|negative regulation of gene expression, epigenetic|
|peptidyl-lysine methylation|
|histone methylation|
|telomere organization|
|regulation of DNA binding|
|regulation of receptor signaling pathway via JAK-STAT|
|DNA damage checkpoint|
|protein alkylation|
|protein methylation|
|regulation of receptor signaling pathway via STAT|
|DNA integrity checkpoint|
|gene silencing|
|cell cycle checkpoint|
|regulation of gene expression, epigenetic|
|macromolecule methylation|
|methylation|
|peptidyl-lysine modification|
|histone modification|
|covalent chromatin modification|
|regulation of binding|
|negative regulation of cell cycle|
|protein-containing complex|
|intracellular membrane-bounded organelle|
|chromatin organization|
|cellular response to DNA damage stimulus|
|peptidyl-amino acid modification|
|positive regulation of cell population proliferation|
|chromosome organization|
|regulation of cell cycle|
|negative regulation of transcription, DNA-templated|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of RNA metabolic process|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|regulation of cell population proliferation|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|cellular response to stress|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|-2.58|
|[[:results:exp483|FTY720 3μM R08 exp483]]|-2.5|
|[[:results:exp348|Cyclosporin-A 3μM R07 exp348]]|-2.3|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|-2.27|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|-2.26|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|-2.22|
|[[:results:exp452|Azithromycin 100μM R08 exp452]]|-2.17|
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|-2.11|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|-2.1|
|[[:results:exp415|Trichostatin-A 0.06μM R07 exp415]]|-2.06|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-2.05|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|-1.98|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|-1.95|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-1.95|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-1.92|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|-1.74|
|[[:results:exp393|Resminostat 0.5μM R07 exp393]]|-1.72|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|2.84|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mllt10|MLLT10]]|0.521|
|[[:human genes:m:mtf1|MTF1]]|0.472|
|[[:human genes:p:prkar1a|PRKAR1A]]|0.425|
|[[:human genes:p:prr12|PRR12]]|0.407|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2468
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.33 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DOT1L Expression in NALM6 Cells: 7.33'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>