======= DPH3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DPH3
  * **<color #00a2e8>Official Name</color>**: diphthamide biosynthesis 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=285381|285381]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96FX2|Q96FX2]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DPH3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DPH3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608959|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 (EEF2) and which can be ADP- ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta). {ECO:0000250}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|zf-CSL|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|peptidyl-diphthamide biosynthetic process from peptidyl-histidine|
|peptidyl-diphthamide metabolic process|
|peptidyl-histidine modification|
|regulation of cytoplasmic translational elongation|
|tRNA wobble uridine modification|
|regulation of translational elongation|
|tRNA wobble base modification|
|regulation of cytoplasmic translation|
|tRNA modification|
|tRNA processing|
|negative regulation of protein secretion|
|negative regulation of peptide secretion|
|RNA modification|
|positive regulation of binding|
|negative regulation of protein transport|
|negative regulation of establishment of protein localization|
|tRNA metabolic process|
|negative regulation of secretion by cell|
|negative regulation of secretion|
|regulation of translation|
|regulation of binding|
|ncRNA processing|
|regulation of cellular amide metabolic process|
|regulation of protein secretion|
|ncRNA metabolic process|
|negative regulation of transport|
|regulation of peptide secretion|
|posttranscriptional regulation of gene expression|
|regulation of protein transport|
|regulation of peptide transport|
|regulation of establishment of protein localization|
|regulation of secretion by cell|
|regulation of secretion|
|RNA processing|
|peptidyl-amino acid modification|
|regulation of protein localization|
|RNA metabolic process|
|positive regulation of molecular function|
|regulation of transport|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-2.25|
|[[:results:exp59|UMK57 1μM R01 exp59]]|-1.86|
|[[:results:exp355|Dinaciclib 0.007μM R07 exp355]]|-1.8|
|[[:results:exp131|L-74142 5μM R03 exp131]]|-1.73|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|1.71|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.71|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|1.72|
|[[:results:exp474|CR131-b 0.005μM R08 exp474]]|1.76|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|1.76|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|1.85|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|1.93|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|1.94|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|1.98|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|1.99|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|2.15|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|2.31|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|2.36|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|2.49|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|2.52|
|[[:results:exp116|AICAR 240μM R03 exp116]]|2.68|
|[[:results:exp359|FK-506 30μM R07 exp359]]|2.71|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|3.16|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|3.17|
|[[:results:exp135|MS023 7μM R03 exp135]]|3.73|
|[[:results:exp134|MS023 2μM R03 exp134]]|3.77|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|4.57|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:ctu1|CTU1]]|0.686|
|[[:human genes:e:elp3|ELP3]]|0.67|
|[[:human genes:e:elp6|ELP6]]|0.653|
|[[:human genes:e:elp2|ELP2]]|0.65|
|[[:human genes:m:mocs3|MOCS3]]|0.641|
|[[:human genes:u:urm1|URM1]]|0.635|
|[[:human genes:k:kti12|KTI12]]|0.629|
|[[:human genes:c:ctu2|CTU2]]|0.623|
|[[:human genes:d:dph1|DPH1]]|0.615|
|[[:human genes:i:ikbkap|IKBKAP]]|0.582|
|[[:human genes:d:dph5|DPH5]]|0.535|
|[[:human genes:e:elp5|ELP5]]|0.529|
|[[:human genes:e:elp4|ELP4]]|0.507|
|[[:human genes:n:nr2c2ap|NR2C2AP]]|0.507|
|[[:human genes:d:dnajc24|DNAJC24]]|0.498|
|[[:human genes:d:dph2|DPH2]]|0.483|
|[[:human genes:c:c7orf26|C7orf26]]|0.453|
|[[:human genes:s:sbds|SBDS]]|0.418|
|[[:human genes:t:thg1l|THG1L]]|0.409|
|[[:human genes:a:alkbh8|ALKBH8]]|0.401|
|[[:human genes:r:rbm26|RBM26]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 576/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|19/28|
|blood|15/28|
|bone|22/26|
|breast|23/33|
|central nervous system|49/56|
|cervix|3/4|
|colorectal|13/17|
|esophagus|10/13|
|fibroblast|1/1|
|gastric|12/16|
|kidney|21/21|
|liver|16/20|
|lung|52/75|
|lymphocyte|11/16|
|ovary|19/26|
|pancreas|21/24|
|peripheral nervous system|14/16|
|plasma cell|6/15|
|prostate|1/1|
|skin|23/24|
|soft tissue|8/9|
|thyroid|1/2|
|upper aerodigestive|19/22|
|urinary tract|26/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1514
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.34 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DPH3 Expression in NALM6 Cells: 4.34'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>