======= DPH5 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DPH5
  * **<color #00a2e8>Official Name</color>**: diphthamide biosynthesis 5
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=51611|51611]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9H2P9|Q9H2P9]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DPH5&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DPH5|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/611075|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis. {ECO:0000250|UniProtKB:P32469, ECO:0000269|PubMed:23486472}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|TP methylase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|diphthine synthase activity|
|peptidyl-diphthamide metabolic process|
|peptidyl-diphthamide biosynthetic process from peptidyl-histidine|
|peptidyl-histidine modification|
|regulation of cytoplasmic translational elongation|
|regulation of translational elongation|
|regulation of cytoplasmic translation|
|methylation|
|regulation of translation|
|regulation of cellular amide metabolic process|
|cellular component|
|posttranscriptional regulation of gene expression|
|peptidyl-amino acid modification|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|-2.81|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|-2.48|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-2.3|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|-2.27|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-2.27|
|[[:results:exp423|Zebularine 20μM R07 exp423]]|-2.05|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|-2|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|-1.96|
|[[:results:exp481|Ethambutol 25μM R08 exp481]]|-1.85|
|[[:results:exp169|BH1 1μM R04 exp169]]|-1.75|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|1.72|
|[[:results:exp390|Pifithrin-alpha 20μM R07 exp390]]|1.72|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|1.75|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|1.78|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|1.79|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|1.79|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|1.79|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|1.81|
|[[:results:exp211|AICAR 240μM R05 exp211]]|1.82|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|1.84|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|1.87|
|[[:results:exp45|Docetaxel 0.002μM R01 exp45]]|1.9|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|1.91|
|[[:results:exp120|Dimethyl-Sulfoxide 1pc R03 exp120]]|1.93|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|1.95|
|[[:results:exp359|FK-506 30μM R07 exp359]]|1.96|
|[[:results:exp534|Trientine 500μM R08 exp534]]|1.96|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|1.97|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|2|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|2.02|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|2.04|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|2.05|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|2.28|
|[[:results:exp275|Citral 75μM R06 exp275]]|2.31|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|2.35|
|[[:results:exp103|Taxol 0.004μM R03 exp103]]|2.41|
|[[:results:exp116|AICAR 240μM R03 exp116]]|2.54|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|2.57|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.78|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|2.8|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|2.86|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|3.04|
|[[:results:exp135|MS023 7μM R03 exp135]]|3.25|
|[[:results:exp274|Citral 50μM R06 exp274]]|3.3|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|3.4|
|[[:results:exp134|MS023 2μM R03 exp134]]|3.4|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|4.01|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:d:dph1|DPH1]]|0.759|
|[[:human genes:d:dph6|DPH6]]|0.694|
|[[:human genes:d:dnajc24|DNAJC24]]|0.692|
|[[:human genes:d:dph2|DPH2]]|0.685|
|[[:human genes:d:dph3|DPH3]]|0.535|
|[[:human genes:m:mapkap1|MAPKAP1]]|0.474|
|[[:human genes:u:urm1|URM1]]|0.469|
|[[:human genes:e:elp2|ELP2]]|0.458|
|[[:human genes:m:mocs3|MOCS3]]|0.457|
|[[:human genes:p:pik3cd|PIK3CD]]|0.456|
|[[:human genes:c:ctu1|CTU1]]|0.437|
|[[:human genes:c:cd81|CD81]]|0.437|
|[[:human genes:k:kti12|KTI12]]|0.437|
|[[:human genes:c:cd19|CD19]]|0.428|
|[[:human genes:n:nr2c2ap|NR2C2AP]]|0.427|
|[[:human genes:e:elp6|ELP6]]|0.424|
|[[:human genes:c:cxorf56|CXorf56]]|0.417|
|[[:human genes:y:ythdf2|YTHDF2]]|0.416|
|[[:human genes:e:elp3|ELP3]]|0.415|
|[[:human genes:z:zzz3|ZZZ3]]|0.411|
|[[:human genes:p:prim1|PRIM1]]|0.401|
|[[:human genes:s:slc7a1|SLC7A1]]|0.4|
|[[:human genes:k:kdsr|KDSR]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 5/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|2/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9812
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.29 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DPH5 Expression in NALM6 Cells: 5.29'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>