======= GBF1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GBF1
  * **<color #00a2e8>Official Name</color>**: golgi brefeldin A resistant guanine nucleotide exchange factor 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=8729|8729]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q92538|Q92538]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GBF1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GBF1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603698|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891). {ECO:0000250|UniProtKB:Q9R1D7, ECO:0000269|PubMed:12047556, ECO:0000269|PubMed:12808027, ECO:0000269|PubMed:15616190, ECO:0000269|PubMed:16926190, ECO:0000269|PubMed:17666033, ECO:0000269|PubMed:17956946, ECO:0000269|PubMed:18003980, ECO:0000269|PubMed:18063581, ECO:0000269|PubMed:19461073, ECO:0000269|PubMed:22185782, ECO:0000269|PubMed:22573891, ECO:0000269|PubMed:23386609, ECO:0000269|PubMed:23418352, ECO:0000269|PubMed:24213530, ECO:0000305|PubMed:19039328, ECO:0000305|PubMed:22573891}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Sec7|
|Sec7 N|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|protein localization to endoplasmic reticulum tubular network|
|endoplasmic reticulum-Golgi intermediate compartment organization|
|Golgi transport vesicle coating|
|Golgi disassembly|
|COPI-coated vesicle budding|
|COPI coating of Golgi vesicle|
|protein localization to endoplasmic reticulum exit site|
|organelle inheritance|
|Golgi inheritance|
|Golgi to endosome transport|
|regulation of ARF protein signal transduction|
|ARF guanyl-nucleotide exchange factor activity|
|establishment of monopolar cell polarity|
|establishment or maintenance of monopolar cell polarity|
|reactive oxygen species biosynthetic process|
|phosphatidylinositol-3,5-bisphosphate binding|
|protein localization to Golgi apparatus|
|cellular response to virus|
|Golgi stack|
|regulation of protein localization to cell surface|
|phosphatidylinositol-3,4,5-trisphosphate binding|
|cell leading edge|
|cis-Golgi network|
|organelle disassembly|
|vesicle coating|
|vesicle targeting, to, from or within Golgi|
|endoplasmic reticulum-Golgi intermediate compartment|
|Golgi vesicle budding|
|lipid droplet|
|neutrophil chemotaxis|
|retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum|
|granulocyte chemotaxis|
|vesicle targeting|
|neutrophil migration|
|retrograde transport, endosome to Golgi|
|vesicle budding from membrane|
|granulocyte migration|
|post-Golgi vesicle-mediated transport|
|guanyl-nucleotide exchange factor activity|
|peroxisome|
|reactive oxygen species metabolic process|
|establishment of cell polarity|
|myeloid leukocyte migration|
|Golgi organization|
|protein localization to endoplasmic reticulum|
|leukocyte chemotaxis|
|cytosolic transport|
|trans-Golgi network|
|establishment of vesicle localization|
|vesicle localization|
|establishment or maintenance of cell polarity|
|cell chemotaxis|
|endoplasmic reticulum to Golgi vesicle-mediated transport|
|endosomal transport|
|regulation of Ras protein signal transduction|
|response to virus|
|vesicle organization|
|endoplasmic reticulum lumen|
|regulation of small GTPase mediated signal transduction|
|establishment of organelle localization|
|Golgi vesicle transport|
|leukocyte migration|
|cellular component disassembly|
|endomembrane system organization|
|regulation of cellular protein localization|
|chemotaxis|
|taxis|
|organelle localization|
|Golgi membrane|
|cell activation involved in immune response|
|regulation of mitotic cell cycle|
|viral process|
|protein localization to organelle|
|symbiotic process|
|interspecies interaction between organisms|
|membrane organization|
|regulation of cellular localization|
|cell migration|
|Golgi apparatus|
|regulation of protein localization|
|cell activation|
|localization of cell|
|cell motility|
|immune effector process|
|regulation of cell cycle|
|mitochondrion|
|response to other organism|
|response to external biotic stimulus|
|locomotion|
|response to biotic stimulus|
|protein transport|
|intracellular transport|
|peptide transport|
|movement of cell or subcellular component|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|nitrogen compound transport|
|immune response|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-2.18|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|-2.07|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|-1.92|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|-1.87|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|-1.82|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|-1.79|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-1.71|
|[[:results:exp232|Epothilone-D 0.004 to 0.006μM on day4 R05 exp232]]|1.84|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:chd4|CHD4]]|0.438|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.437|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.437|
|[[:human genes:d:dtl|DTL]]|0.435|
|[[:human genes:r:ranbp2|RANBP2]]|0.434|
|[[:human genes:p:psmc2|PSMC2]]|0.416|
|[[:human genes:c:chmp2a|CHMP2A]]|0.416|
|[[:human genes:t:twistnb|TWISTNB]]|0.41|
|[[:human genes:c:cbwd3|CBWD3]]|0.41|
|[[:human genes:s:syt9|SYT9]]|0.408|
|[[:human genes:d:dync1i2|DYNC1I2]]|0.408|
|[[:human genes:t:tyms|TYMS]]|0.404|
|[[:human genes:a:aatf|AATF]]|0.403|
|[[:human genes:n:nupl1|NUPL1]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 318/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|0/1|
|bile duct|15/28|
|blood|14/28|
|bone|9/26|
|breast|18/33|
|central nervous system|25/56|
|cervix|2/4|
|colorectal|8/17|
|esophagus|3/13|
|fibroblast|0/1|
|gastric|7/16|
|kidney|4/21|
|liver|7/20|
|lung|33/75|
|lymphocyte|9/16|
|ovary|12/26|
|pancreas|4/24|
|peripheral nervous system|14/16|
|plasma cell|7/15|
|prostate|1/1|
|skin|10/24|
|soft tissue|6/9|
|thyroid|1/2|
|upper aerodigestive|5/22|
|urinary tract|5/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2015
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.92 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GBF1 Expression in NALM6 Cells: 6.92'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>