======= GEN1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GEN1
  * **<color #00a2e8>Official Name</color>**: GEN1 Holliday junction 5' flap endonuclease
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=348654|348654]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q17RS7|Q17RS7]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GEN1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GEN1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/612449|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in resolution of Holliday junctions, which are intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. The protein resolves Holliday junctions by creating dual incisions across the junction to produce nicked duplex products that can be ligated. In addition, this protein has been found to localize to centrosomes where it has been implicated in regulation of centrosome integrity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. {ECO:0000269|PubMed:19020614}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|XPG I|
|XPG N|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|resolution of recombination intermediates|
|resolution of mitotic recombination intermediates|
|flap endonuclease activity|
|5-3 exodeoxyribonuclease activity|
|positive regulation of mitotic cell cycle spindle assembly checkpoint|
|positive regulation of spindle checkpoint|
|5-flap endonuclease activity|
|crossover junction endodeoxyribonuclease activity|
|positive regulation of cell cycle checkpoint|
|regulation of mitotic cell cycle spindle assembly checkpoint|
|regulation of spindle checkpoint|
|regulation of mitotic spindle checkpoint|
|four-way junction DNA binding|
|mitotic recombination|
|regulation of cell cycle checkpoint|
|replication fork processing|
|DNA-dependent DNA replication maintenance of fidelity|
|regulation of centrosome duplication|
|regulation of mitotic metaphase/anaphase transition|
|regulation of metaphase/anaphase transition of cell cycle|
|regulation of mitotic sister chromatid separation|
|regulation of centrosome cycle|
|regulation of chromosome separation|
|regulation of mitotic sister chromatid segregation|
|regulation of sister chromatid segregation|
|double-strand break repair via homologous recombination|
|recombinational repair|
|regulation of chromosome segregation|
|DNA-dependent DNA replication|
|positive regulation of mitotic cell cycle|
|regulation of mitotic nuclear division|
|double-strand break repair|
|regulation of microtubule cytoskeleton organization|
|regulation of nuclear division|
|DNA replication|
|magnesium ion binding|
|DNA recombination|
|regulation of microtubule-based process|
|positive regulation of cell cycle process|
|nucleic acid phosphodiester bond hydrolysis|
|regulation of chromosome organization|
|positive regulation of cell cycle|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|centrosome|
|DNA repair|
|regulation of cytoskeleton organization|
|regulation of mitotic cell cycle|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|protein homodimerization activity|
|regulation of cell cycle|
|regulation of organelle organization|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|-2.3|
|[[:results:exp348|Cyclosporin-A 3μM R07 exp348]]|-2.02|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-2|
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|-1.85|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:cct7|CCT7]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 6285
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.32 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GEN1 Expression in NALM6 Cells: 8.32'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>