======= GFI1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GFI1
  * **<color #00a2e8>Official Name</color>**: growth factor independent 1 transcriptional repressor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2672|2672]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q99684|Q99684]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GFI1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GFI1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600871|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2- GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin- mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1- dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells. {ECO:0000269|PubMed:11060035, ECO:0000269|PubMed:12778173, ECO:0000269|PubMed:16287849, ECO:0000269|PubMed:17197705, ECO:0000269|PubMed:17646546, ECO:0000269|PubMed:18805794, ECO:0000269|PubMed:19026687, ECO:0000269|PubMed:19164764, ECO:0000269|PubMed:20190815, ECO:0000269|PubMed:20547752, ECO:0000269|PubMed:8754800}.

<button type='default' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
No Pfam Domain information is available for this gene.
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of interleukin-6-mediated signaling pathway|
|regulation of interleukin-6-mediated signaling pathway|
|negative regulation of calcidiol 1-monooxygenase activity|
|negative regulation of vitamin D biosynthetic process|
|negative regulation of vitamin metabolic process|
|regulation of calcidiol 1-monooxygenase activity|
|regulation of vitamin D biosynthetic process|
|negative regulation of hormone biosynthetic process|
|negative regulation of monooxygenase activity|
|negative regulation of hormone metabolic process|
|negative regulation of alcohol biosynthetic process|
|regulation of vitamin metabolic process|
|negative regulation of steroid biosynthetic process|
|negative regulation of oxidoreductase activity|
|negative regulation of steroid metabolic process|
|regulation of hormone biosynthetic process|
|regulation of histone H3-K4 methylation|
|regulation of transcription involved in G1/S transition of mitotic cell cycle|
|regulation of hormone metabolic process|
|negative regulation of lipid biosynthetic process|
|positive regulation of cytokine-mediated signaling pathway|
|regulation of monooxygenase activity|
|transcriptional repressor complex|
|positive regulation of response to cytokine stimulus|
|regulation of histone methylation|
|regulation of toll-like receptor signaling pathway|
|regulation of alcohol biosynthetic process|
|negative regulation of lipid metabolic process|
|negative regulation of NF-kappaB transcription factor activity|
|regulation of steroid biosynthetic process|
|negative regulation of small molecule metabolic process|
|regulation of oxidoreductase activity|
|nuclear matrix|
|regulation of steroid metabolic process|
|G1/S transition of mitotic cell cycle|
|cell cycle G1/S phase transition|
|regulation of histone modification|
|negative regulation of neuron projection development|
|negative regulation of DNA-binding transcription factor activity|
|regulation of cytokine-mediated signaling pathway|
|regulation of response to cytokine stimulus|
|negative regulation of cell projection organization|
|cellular response to lipopolysaccharide|
|regulation of lipid biosynthetic process|
|regulation of chromatin organization|
|cellular response to molecule of bacterial origin|
|cellular response to biotic stimulus|
|transcription regulatory region DNA binding|
|negative regulation of neuron differentiation|
|DNA-binding transcription repressor activity, RNA polymerase II-specific|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|nuclear body|
|negative regulation of neurogenesis|
|negative regulation of nervous system development|
|response to lipopolysaccharide|
|response to molecule of bacterial origin|
|negative regulation of cell development|
|regulation of chromosome organization|
|regulation of lipid metabolic process|
|regulation of DNA-binding transcription factor activity|
|regulation of small molecule metabolic process|
|regulation of innate immune response|
|regulation of neuron projection development|
|cellular response to lipid|
|regulation of response to biotic stimulus|
|regulation of hormone levels|
|hemopoiesis|
|mitotic cell cycle process|
|hematopoietic or lymphoid organ development|
|immune system development|
|regulation of neuron differentiation|
|DNA-binding transcription factor activity|
|mitotic cell cycle|
|response to bacterium|
|regulation of plasma membrane bounded cell projection organization|
|viral process|
|negative regulation of cellular component organization|
|regulation of cell projection organization|
|negative regulation of cell differentiation|
|regulation of defense response|
|regulation of multi-organism process|
|symbiotic process|
|negative regulation of catalytic activity|
|regulation of neurogenesis|
|interspecies interaction between organisms|
|response to lipid|
|negative regulation of transcription by RNA polymerase II|
|regulation of nervous system development|
|regulation of cell development|
|negative regulation of developmental process|
|cell cycle process|
|cellular response to oxygen-containing compound|
|regulation of response to external stimulus|
|negative regulation of molecular function|
|regulation of immune response|
|negative regulation of transcription, DNA-templated|
|negative regulation of multicellular organismal process|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|regulation of organelle organization|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|negative regulation of RNA metabolic process|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|regulation of response to stress|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|DNA-binding transcription factor activity, RNA polymerase II-specific|
|neurogenesis|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of immune system process|
|positive regulation of signal transduction|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.34|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|-2.3|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.08|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-1.88|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.76|
|[[:results:exp467|CAY10603 0.55μM R08 exp467]]|-1.74|
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|1.79|
|[[:results:exp286|HMS-I2 1μM R06 exp286]]|1.98|
|[[:results:exp149|SB203580 25μM R03 exp149]]|1.99|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|2.04|
|[[:results:exp484|GSK-J5 1.5μM R08 exp484]]|2.05|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.413|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 2/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 12870
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 3.71 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GFI1 Expression in NALM6 Cells: 3.71'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>